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公开(公告)号：KR1019900004219B1
公开(公告)日：1990-06-18
申请号：KR1019870015571
申请日：1987-12-31
Applicant: 한국과학기술연구원
Abstract: A process for extg. DNA from alaskan pollack comprises (a) pulverizing spermatogonia sepd. from alaskan pollack on a stirrer to obtain colloid, (b) screening with a 2 mm sieve, (c) dissolving in a physiological saline soln., (d) centrifugating to sep. cell nuclei of milk-white, (e) adding anionic surfactant i.e. sodium dodecylsulfomate (SDS) to metamorphose nuclear membrane and nuclear protein, (f) sepg. histones with 2N NaCl, (g) adding isopropanol of 1/2 vol. to sep. fibrous material, and (h) washing with acetone and drying
Abstract translation: 一个extg的过程 来自阿拉斯加鳕鱼的DNA包括（a）粉碎精原细胞。 在搅拌器上从阿拉斯加鳕鱼获得胶体，（b）用2mm筛选筛选，（c）溶解在生理盐水溶液中，（d）离心至sep。 乳白色细胞核，（e）将阴离子表面活性剂即十二烷基磺酸钠（SDS）加入到变性核膜和核蛋白中，（f）sepg。 用2N NaCl组蛋白，（g）加入1/2体积的异丙醇 到sep 纤维材料，和（h）用丙酮洗涤并干燥
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