명태의 정원세포로부터 DNA의 추출방법
    3.
    发明授权
    명태의 정원세포로부터 DNA의 추출방법 失效
    精原细胞DNA提取方法

    公开(公告)号:KR1019900004219B1

    公开(公告)日:1990-06-18

    申请号:KR1019870015571

    申请日:1987-12-31

    Abstract: A process for extg. DNA from alaskan pollack comprises (a) pulverizing spermatogonia sepd. from alaskan pollack on a stirrer to obtain colloid, (b) screening with a 2 mm sieve, (c) dissolving in a physiological saline soln., (d) centrifugating to sep. cell nuclei of milk-white, (e) adding anionic surfactant i.e. sodium dodecylsulfomate (SDS) to metamorphose nuclear membrane and nuclear protein, (f) sepg. histones with 2N NaCl, (g) adding isopropanol of 1/2 vol. to sep. fibrous material, and (h) washing with acetone and drying

    Abstract translation: 一个extg的过程 来自阿拉斯加鳕鱼的DNA包括(a)粉碎精原细胞。 在搅拌器上从阿拉斯加鳕鱼获得胶体,(b)用2mm筛选筛选,(c)溶解在生理盐水溶液中,(d)离心至sep。 乳白色细胞核,(e)将阴离子表面活性剂即十二烷基磺酸钠(SDS)加入到变性核膜和核蛋白中,(f)sepg。 用2N NaCl组蛋白,(g)加入1/2体积的异丙醇 到sep 纤维材料,和(h)用丙酮洗涤并干燥

    효소의 역가수분해반응을 이용한 당유도체의 제조방법
    4.
    发明授权
    효소의 역가수분해반응을 이용한 당유도체의 제조방법 失效
    制备用于使用酶的逆水解的盐酸衍生物的方法

    公开(公告)号:KR1019910008732B1

    公开(公告)日:1991-10-19

    申请号:KR1019900000740

    申请日:1990-01-22

    Inventor: 이대실 박한오

    Abstract: A process for preparing saccharide deriv. of formula (I) [R is methyl, ethyl, isopropyl or monosacchride comprises: (a) mixing one or more organic solvents with alkyl boric acid, and dissolving carbohydrate; (b) treating the soln. with glycosidase at 15-90 deg.C and pH 3-10 for forming glycoside bond to obtain the final product. The carbohydrate is mono-, di- or polysaccharide. The organic solvent is petroleum ether, hexane, octane, toluene, benzene, chloroform, isopropylether, ethylacetate, cyclohexanone, tetrahydrofuran, dioxane, acetone, etc.. The glycosidase is glucosidase, galactosidase or mannosidase.

    Abstract translation: 制备糖衍生物的方法。 式(I)的化合物[R是甲基,乙基,异丙基或单糖包括:(a)将一种或多种有机溶剂与烷基硼酸混合并溶解碳水化合物; (b)处理溶胶。 与糖苷酶在15-90℃和pH 3-10形成糖苷键以获得最终产物。 碳水化合物是单糖,二糖或多糖。 有机溶剂为石油醚,己烷,辛烷,甲苯,苯,氯仿,异丙醚,乙酸乙酯,环己酮,四氢呋喃,二恶烷,丙酮等。糖苷酶为葡糖苷酶,半乳糖苷酶或甘露糖苷酶。

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