公开(公告)号：KR1020020042882A

公开(公告)日：2002-06-08

申请号：KR1020000072220

申请日：2000-12-01

Applicant: 한국과학기술연구원

Inventor： 김기선 ,  정의준 ,  방숙희

IPC: C07K14/47

CPC classification number: C07K14/4703

Abstract: PURPOSE: The FADD-DD mutant proteins and genes encoding for the same are provided, thereby the FADD-DD mutant proteins capable of selectively transferring the cell death signal of Fas or TNF-alpha(tumor necrosis factor-alpha) can be prepared and the proteins can be effectively used in study on chemical therapy and cell death by stress. CONSTITUTION: The FADD-DD(Fas-associated death domain protein-death domain) mutant protein has the amino acid sequence of which 110th amino acid is substituted with an amino acid selected from glycine, alanine, valine, leucine and isoleucine. The FADD-DD mutant protein R110A, wherein 110th amino acid of the FADD-DD protein is substituted with alanine, has the amino acid sequence of SEQ ID NO: 11. A gene encoding for the FADD-DD mutant protein R110A has the nucleotide sequence of SEQ ID NO: 5. The FADD-DD mutant protein has the amino acid sequence of which 117th amino acid is substituted with an amino acid selected from glycine, alanine, valine, leucine and isoleucine. The FADD-DD mutant protein R117A, wherein 117th amino acid of the FADD-DD protein is substituted with alanine, has the amino acid sequence of SEQ ID NO: 12. A gene encoding for the FADD-DD mutant protein R117A has the nucleotide sequence of SEQ ID NO: 7.

Abstract translation: 目的：提供FADD-DD突变蛋白和编码其的基因，从而可以制备能够选择性转移Fas或TNF-α（肿瘤坏死因子-α）细胞死亡信号的FADD-DD突变蛋白， 蛋白质可以有效地用于化学治疗和细胞死亡应激的研究。 构成：FADD-DD（Fas相关的死亡域蛋白 - 死亡域）突变蛋白具有氨基酸序列，其第110位氨基酸被选自甘氨酸，丙氨酸，缬氨酸，亮氨酸和异亮氨酸的氨基酸取代。 FADD-DD突变蛋白R110A，其中FADD-DD蛋白质的第110位氨基酸被丙氨酸取代，具有SEQ ID NO：11的氨基酸序列。编码FADD-DD突变蛋白R110A的基因具有核苷酸序列 的FADD-DD突变蛋白具有第117位氨基酸被选自甘氨酸，丙氨酸，缬氨酸，亮氨酸和异亮氨酸的氨基酸取代的氨基酸序列。 FADD-DD突变蛋白R117A，其中FADD-DD蛋白的第117位氨基酸被丙氨酸取代，具有SEQ ID NO：12的氨基酸序列。编码FADD-DD突变蛋白R117A的基因具有核苷酸序列 的SEQ ID NO：7。