公开(公告)号：KR1020080089726A

公开(公告)日：2008-10-08

申请号：KR1020070032247

申请日：2007-04-02

Applicant: 한국과학기술연구원

Inventor： 강지윤 ,  채수경 ,  김태송 ,  이상호 ,  양인홍

IPC: C12N5/00 ,  C12N5/07 ,  A61K38/39

Abstract: A method for preparation of extracellular matrix is provided to improve cell adhesion, cell growth rate and cell differentiation ability, and mass produce the extracellular matrix by dissolving egg membrane by using pronase as a protein-decomposing enzyme. A method for preparation of extracellular matrix comprises the steps of: isolating the egg membrane from embryo of mammals such as zebrafish; treating the isolated egg membrane with pronase as a protein-decomposing enzyme; and inactivating the pronase with an inactivating agent such as urea containing beta-mercaptoethanol(BME) and ethylene diamine tetraacetic acid(EDTA) or by heating it. Further, the method for preparation of extracellular matrix additionally comprises a step of inactivating the pronase by heating the pronase.

Abstract translation: 提供了一种制备细胞外基质的方法，以改善细胞粘附，细胞生长速度和细胞分化能力，通过使用链霉蛋白酶作为蛋白质分解酶溶解卵膜，大量产生细胞外基质。 制备细胞外基质的方法包括以下步骤：从哺乳动物如斑马鱼的胚胎中分离卵膜; 用分离蛋白酶分解酶处理分离的卵膜; 并用灭活剂如含有β-巯基乙醇（BME）和乙二胺四乙酸（EDTA）的尿素或通过加热来灭活链霉蛋白酶。 此外，细胞外基质的制备方法另外包括通过加热链霉蛋白酶来灭活链霉蛋白酶的步骤。