유-무기 하이브리드 그라프트 폴리실세스퀴옥산의 제조 방법 및 이에 의하여 제조된 그라프트 폴리실세스퀴옥산
    3.
    发明公开
    유-무기 하이브리드 그라프트 폴리실세스퀴옥산의 제조 방법 및 이에 의하여 제조된 그라프트 폴리실세스퀴옥산 无效
    有机无机混合物多晶硅烷的制备方法及其制备的多晶硅烷氧基硅烷的制备方法

    公开(公告)号:KR1020100131904A

    公开(公告)日:2010-12-16

    申请号:KR1020090091239

    申请日:2009-09-25

    Abstract: PURPOSE: A method for preparing organic-inorganic hybrid graft polysilsesquioxane is provided to obtain polysilsesquioxanes with a highly functioned ladder structure by easily grafting various functional monomers to a side chain of polysilsesquioxane. CONSTITUTION: A method for preparing organic-inorganic hybrid graft polysilsesquioxane comprises a step for polymerizing one or more kinds of an unsaturated hydrocarbon-based monomer to a side chain of polysilsesquioxane represented by chemical formula 1 to form polysilsesquioxane with which a homopolymer or copolymer with the unsaturated hydrocarbon-based monomer at a side chain is grafted.

    Abstract translation: 目的:提供一种制备有机 - 无机杂化接枝聚倍半硅氧烷的方法,通过容易地将各种官能单体接枝到聚倍半硅氧烷的侧链,获得具有高功能梯形结构的聚倍半硅氧烷。 构成:用于制备有机 - 无机杂化接枝聚倍半硅氧烷的方法包括将一种或多种不饱和烃类单体聚合到由化学式1表示的聚倍半硅氧烷的侧链上以形成聚倍半硅氧烷的步骤,通过其与均聚物或共聚物与 侧链上的不饱和烃类单体被接枝。

    난막을 단백질 분해효소로 처리하여 세포외기질을 제조하는방법 및 그 인공 세포외기질
    4.
    发明公开
    난막을 단백질 분해효소로 처리하여 세포외기질을 제조하는방법 및 그 인공 세포외기질 无效
    通过EGG膜和人造纤维细胞基质的蛋白质去除制备细胞外基质的方法

    公开(公告)号:KR1020080089726A

    公开(公告)日:2008-10-08

    申请号:KR1020070032247

    申请日:2007-04-02

    Abstract: A method for preparation of extracellular matrix is provided to improve cell adhesion, cell growth rate and cell differentiation ability, and mass produce the extracellular matrix by dissolving egg membrane by using pronase as a protein-decomposing enzyme. A method for preparation of extracellular matrix comprises the steps of: isolating the egg membrane from embryo of mammals such as zebrafish; treating the isolated egg membrane with pronase as a protein-decomposing enzyme; and inactivating the pronase with an inactivating agent such as urea containing beta-mercaptoethanol(BME) and ethylene diamine tetraacetic acid(EDTA) or by heating it. Further, the method for preparation of extracellular matrix additionally comprises a step of inactivating the pronase by heating the pronase.

    Abstract translation: 提供了一种制备细胞外基质的方法,以改善细胞粘附,细胞生长速度和细胞分化能力,通过使用链霉蛋白酶作为蛋白质分解酶溶解卵膜,大量产生细胞外基质。 制备细胞外基质的方法包括以下步骤:从哺乳动物如斑马鱼的胚胎中分离卵膜; 用分离蛋白酶分解酶处理分离的卵膜; 并用灭活剂如含有β-巯基乙醇(BME)和乙二胺四乙酸(EDTA)的尿素或通过加热来灭活链霉蛋白酶。 此外,细胞外基质的制备方法另外包括通过加热链霉蛋白酶来灭活链霉蛋白酶的步骤。

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