公开(公告)号：KR1020040006307A

公开(公告)日：2004-01-24

申请号：KR1020020040325

申请日：2002-07-11

Applicant: 한국과학기술연구원 ,  한올바이오파마주식회사 ,  경동제약 주식회사

Inventor： 정찬성 ,  한예선 ,  윤문영 ,  조현성 ,  이병석

IPC: C12P17/04

Abstract: PURPOSE: A process for preparing cis-2- (bromomethyl)-2- (2,4-dichlorophenyl)- 13-dioxolane- 4-methyl alcohol having high optical selectivity is provided. A stereo-selective enzyme maintains its maximum activity because the reaction product is rapidly removed from the reaction solution during the reaction in order not to inhibit the activity of the enzyme. CONSTITUTION: A process for preparing cis-2- (bromomethyl)-2- (2,4-dichlorophenyl)- 13-dioxolane- 4-methyl alcohol having high optical selectivity comprises hydrolysis of cis-2- (bromomethyl)-2- (2,4-dichlorophenyl)- 1,3-dioxolane- 4-methyl acetate using lipase produced from Acinetobacter sp. SY-01(KFCC-11111), and recovering an alcohol product as a reaction product from the reaction solution during the reaction, wherein the concentration of the alcohol product is 0.1 to 300 mg/l; the reaction product is recovered when the conversion rate of the hydrolysis reaction is 40 to 60%; the hydrolysis reaction is carried out under conditions of 40 to 60 deg. C, pH 7.0 to 9.0 and 100 to 300 rpm; and the reaction product is recovered by column chromatography.

Abstract translation: 目的：提供具有高光学选择性的顺式-2-（溴甲基）-2-（2,4-二氯苯基）-1,3-二氧戊环-4-甲醇的方法。 立体选择性酶维持其最大活性，因为在反应期间快速从反应溶液中除去反应产物，以便不抑制酶的活性。 构成：具有高光学选择性的顺式-2-（溴甲基）-2-（2,4-二氯苯基）-1,3-二氧戊环-4-甲醇的制备方法包括顺式-2-（溴甲基）-2-（ 2,4-二氯苯基）-1,3-二氧戊环-4-甲基乙酸酯，使用由不动杆菌属（Acinetobacter sp。）生产的脂肪酶。 SY-01（KFCC-11111），并且在反应期间从反应溶液中回收作为反应产物的醇产物，其中醇产物的浓度为0.1至300mg / l; 当水解反应的转化率为40〜60％时回收反应产物; 水解反应在40〜60℃的条件下进行。 C，pH 7.0至9.0和100至300rpm; 并通过柱色谱法回收反应产物。

公开(公告)号：KR1020020017066A

公开(公告)日：2002-03-07

申请号：KR1020000050149

申请日：2000-08-28

Applicant: 한국과학기술연구원

Inventor： 신평균 ,  윤문영 ,  박호일 ,  정찬성 ,  한예선

IPC: C12N1/20

Abstract: PURPOSE: Provided is a microorganism strain producing thermophile lipase. The above strain is obtained from the soil samples near the industrial complex, identified as a novel lipase producing strain different from known Burkholderia cepacia and named as SY-02(Burkholderia cepacia SY-02). It shows thermo-stability and high enzyme activity at the higher temperature than the melting point of fat and is thus widely useful as a lipase producing strain in the field of industry. CONSTITUTION: The strain Burkholderia cepacis SY-02(KFCC-111210) produces thermophilic lipase which is secreted into the exterior of a cell of Burkholderia cepacia SY-02, and shows activity at 40-80 deg.C, preferably 70 deg.C.

Abstract translation: 目的：提供一种生产嗜热脂肪酶的微生物菌株。 上述菌株从工业化合物附近的土壤样品中获得，鉴定为与已知的洋葱伯克霍尔德菌（Burkholderia cepacia）不同的新型脂肪酶生产菌株，命名为SY-02（洋葱伯克霍尔德菌（SYK））。 它在比脂肪的熔点高的温度下显示出热稳定性和高酶活性，因此在工业领域中广泛用作脂肪酶生产菌株。 构成：洋葱伯克霍尔德菌SY-02（KFCC-111210）产生嗜热脂肪酶，其分泌到洋葱伯克霍尔德菌SY-02细胞的外部，并在40-80℃，优选70℃显示出活性。

公开(公告)号：KR1020010109632A

公开(公告)日：2001-12-12

申请号：KR1020000029739

申请日：2000-05-31

Applicant: 한국과학기술연구원 ,  한올바이오파마주식회사 ,  경동제약 주식회사

Inventor： 신평균 ,  윤문영 ,  박호일 ,  배희경 ,  정찬성 ,  한예선 ,  박정극 ,  이병석 ,  김순일

IPC: C12N9/16

CPC classification number: C12N9/16 ,  C07K1/14

Abstract: 본발명은 신균주 벌콜데리아 코코베네난스(Burkholderia Cocovenenans) SY-01과 이로부터 생산되는 입체선택적 기질특이성을 갖는 리파제에 관한 것으로, 더욱 상세하게는 항진균제의 원료로 사용되는 이트라코나졸의 중간체로서 라세믹 혼합물인 시스-2-(브로모메틸)-2-(2,4-디클로로 페닐)-1,3-디옥소란-4-메틸 아세테이트의 에스테르결합을 입체선택적으로 분해할 수 있는 리파제에 관한 것이다.

公开(公告)号：KR100446256B1

公开(公告)日：2004-09-01

申请号：KR1020020040325

申请日：2002-07-11

Applicant: 한국과학기술연구원 ,  한올바이오파마주식회사 ,  경동제약 주식회사

Inventor： 정찬성 ,  한예선 ,  윤문영 ,  조현성 ,  이병석

IPC: C12P17/04

Abstract: PURPOSE: A process for preparing cis-2- (bromomethyl)-2- (2,4-dichlorophenyl)- 13-dioxolane- 4-methyl alcohol having high optical selectivity is provided. A stereo-selective enzyme maintains its maximum activity because the reaction product is rapidly removed from the reaction solution during the reaction in order not to inhibit the activity of the enzyme. CONSTITUTION: A process for preparing cis-2- (bromomethyl)-2- (2,4-dichlorophenyl)- 13-dioxolane- 4-methyl alcohol having high optical selectivity comprises hydrolysis of cis-2- (bromomethyl)-2- (2,4-dichlorophenyl)- 1,3-dioxolane- 4-methyl acetate using lipase produced from Acinetobacter sp. SY-01(KFCC-11111), and recovering an alcohol product as a reaction product from the reaction solution during the reaction, wherein the concentration of the alcohol product is 0.1 to 300 mg/l; the reaction product is recovered when the conversion rate of the hydrolysis reaction is 40 to 60%; the hydrolysis reaction is carried out under conditions of 40 to 60 deg. C, pH 7.0 to 9.0 and 100 to 300 rpm; and the reaction product is recovered by column chromatography.

Abstract translation: 目的：提供一种制备光学选择性高的顺式-2-（溴甲基）-2-（2,4-二氯苯基）-13-二氧戊环-4-甲醇的方法。 立体选择性酶保持其最大活性，因为在反应过程中反应产物从反应溶液中快速除去以不抑制酶的活性。 结构：制备具有高光学选择性的顺式-2-（溴甲基）-2-（2,4-二氯苯基）-13-二氧戊环-4-甲醇的方法包括顺式-2-（溴甲基）-2-（ 2,4-二氯苯基）-1,3-二氧戊环-4-甲基乙酸酯使用由不动杆菌产生的脂肪酶进行。 SY-01（KFCC-11111），在反应过程中从反应溶液中回收作为反应产物的醇产物，其中醇产物的浓度为0.1-300mg / l; 当水解反应的转化率为40至60％时，反应产物被回收; 水解反应在40〜60℃的条件下进行。 C，pH 7.0至9.0和100至300rpm; 并通过柱色谱法回收反应产物。

公开(公告)号：KR100411236B1

公开(公告)日：2003-12-18

申请号：KR1020000050149

申请日：2000-08-28

Applicant: 한국과학기술연구원

Inventor： 신평균 ,  윤문영 ,  박호일 ,  정찬성 ,  한예선

IPC: C12N1/20

Abstract: PURPOSE: Provided is a microorganism strain producing thermophile lipase. The above strain is obtained from the soil samples near the industrial complex, identified as a novel lipase producing strain different from known Burkholderia cepacia and named as SY-02(Burkholderia cepacia SY-02). It shows thermo-stability and high enzyme activity at the higher temperature than the melting point of fat and is thus widely useful as a lipase producing strain in the field of industry. CONSTITUTION: The strain Burkholderia cepacis SY-02(KFCC-111210) produces thermophilic lipase which is secreted into the exterior of a cell of Burkholderia cepacia SY-02, and shows activity at 40-80 deg.C, preferably 70 deg.C.

Abstract translation: 目的：提供一种产生嗜热脂肪酶的微生物菌株。 上述菌株从工业园区附近的土壤样品中获得，鉴定为与已知洋葱伯克霍尔德菌不同的新型脂肪酶生产菌株，命名为SY-02（洋葱伯克霍尔德菌SY-02）。 它在高于脂肪熔点的温度下显示热稳定性和高酶活性，因此广泛用作工业领域中的脂肪酶生产菌株。 构成：菌株伯克霍尔德氏菌SY-02（KFCC-111210）产生分泌到洋葱伯克霍尔德氏菌SY-02细胞外部的嗜热性脂肪酶，并在40-80℃，优选70℃显示出活性。

公开(公告)号：KR100373151B1

公开(公告)日：2003-02-25

申请号：KR1020000029739

申请日：2000-05-31

Applicant: 한국과학기술연구원 ,  한올바이오파마주식회사 ,  경동제약 주식회사

Inventor： 신평균 ,  윤문영 ,  박호일 ,  배희경 ,  정찬성 ,  한예선 ,  박정극 ,  이병석 ,  김순일

IPC: C12N9/16

Abstract: 본발명은 신균주 악시네토박터(
Acinetobacter sp. ) SY-01 (상기 균주는 벌콜데리아 코코베네난스(
Burkholderia cocovenenans ) SY-01로 기탁되었으나, 2002년 5월 1일자로 제출한 과학적 성질 및 분류학상의 위치 표시 등의 기록서를 통하여 분류학상의 위치가
Acinetobacter sp. SY-01로 수정되었음)과 이로부터 생산되는 입체선택적 기질특이성을 갖는 리파제에 관한 것으로, 더욱 상세하게는 항진균제의 원료로 사용되는 이트라코나졸의 중간체로서 라세믹 혼합물인 시스-2-(브로모메틸)-2-(2,4-디클로로 페닐)-1,3-디옥소란-4-메틸 아세테이트의 에스테르결합을 입체선택적으로 분해할 수 있는 리파제에 관한 것이다.