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    신규 플라스미드 제조 및 그를 함유한 균주를 배양하여 아이 지 에프-1(IGF-1)을 생산하는 방법
    1.
    发明授权
    신규 플라스미드 제조 및 그를 함유한 균주를 배양하여 아이 지 에프-1(IGF-1)을 생산하는 방법 失效
    生产IGF-I的方法

    公开(公告)号：KR1019920003787B1

    公开(公告)日：1992-05-14

    申请号：KR1019900002811

    申请日：1990-03-05

    Applicant: 한국과학기술연구원

    Inventor： 이영익 ,  곽주원 ,  박희동 ,  임선영 ,  김영훈 ,  윤미선

    IPC: C12N15/17

    CPC classification number: C07K14/65 ,  C07K2319/00 ,  C07K2319/50 ,  C07K2319/61 ,  C07K2319/75

    Abstract: Prepn. of insulin-like growth factor 1 (IGF-1) (I) by transforming an E.Coli strain with an expression vector contg. cDNA encoding (I) (prepd. from mRNA extracted and isolated from human hepatocytes), the cDNA being inserted in the form of a fusion gene with another gene, followed by culture and purificn. of the resulting (I), the improvement comprises utilising an expression vector which is a plasmid having an exterokinase recognition site between the (I) gener and the other gene, and the resulting fusion protein is cleaved with enterokinase. The prod. (I) is used for controlling the blood glucose level in diabetic patients who cannot be treated with insulin, for local treatment of bone fracture and for treating congenitalor acquired growth failure patients.

    Abstract translation: 制备方法。 的胰岛素样生长因子1（IGF-1）（I）通过用表达载体contg转化大肠杆菌菌株。 cDNA编码（I）（从人肝细胞提取和分离的mRNA制备），以与另一基因的融合基因的形式插入cDNA，然后培养和纯化。 所得到的（I）的改进包括利用作为在（I）产生和另一个基因之间具有消旋酶酶识别位点的质粒的表达载体，并且所得融合蛋白用肠激酶切割。 产品 （I）用于控制不能用胰岛素治疗的糖尿病患者的血糖水平，局部治疗骨折和治疗先天性获得性生长衰竭患者。

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