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公开(公告)号：KR1020000002227A
公开(公告)日：2000-01-15
申请号：KR1019980022839
申请日：1998-06-18
Applicant: 한국과학기술연구원
IPC: C07K16/18
Abstract: PURPOSE: The monoclonal antibody mAbB55 and HmAbB128 which are produced by hybridoma strains H-mAbB55 and mAbB128 showing a superior binding specificity to human apolipoprotein B and can be used for diagnosing blood circulatory organ disease. CONSTITUTION: The human apolipoprotein B-100 antibodies are injected into a mouse for immunization of the pancreas and coalesced with myeloma cell for producing hybridoma strains. The binding specificity to apolipoprotein B-100 of monoclonal antibodies, which are produced by hybridoma strains, is analyzed by Western Blatting Method and enzyme immunoassay. The potency of monoclonal antibodies and the concentration of apolipoprotein B-100, which is bound to monoclonal antibodies, are measured by enzyme immune-assay.
Abstract translation: 目的：由杂交瘤菌株H-mAbB55和mAbB128产生的单克隆抗体mAbB55和HmAbB128显示出对人载脂蛋白B的优异结合特异性，可用于诊断血液循环器官疾病。 构成：将人载脂蛋白B-100抗体注射到小鼠中用于胰腺免疫并与骨髓瘤细胞聚结以产生杂交瘤菌株。 通过杂交瘤菌株产生的单克隆抗体载脂蛋白B-100的结合特异性通过Western Blatting Method和酶免疫测定法进行分析。 通过酶免疫测定法测量单克隆抗体的效力和与单克隆抗体结合的载脂蛋白B-100的浓度。
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公开(公告)号：KR1019920003787B1
公开(公告)日：1992-05-14
申请号：KR1019900002811
申请日：1990-03-05
Applicant: 한국과학기술연구원
IPC: C12N15/17
CPC classification number: C07K14/65 , C07K2319/00 , C07K2319/50 , C07K2319/61 , C07K2319/75
Abstract: Prepn. of insulin-like growth factor 1 (IGF-1) (I) by transforming an E.Coli strain with an expression vector contg. cDNA encoding (I) (prepd. from mRNA extracted and isolated from human hepatocytes), the cDNA being inserted in the form of a fusion gene with another gene, followed by culture and purificn. of the resulting (I), the improvement comprises utilising an expression vector which is a plasmid having an exterokinase recognition site between the (I) gener and the other gene, and the resulting fusion protein is cleaved with enterokinase. The prod. (I) is used for controlling the blood glucose level in diabetic patients who cannot be treated with insulin, for local treatment of bone fracture and for treating congenitalor acquired growth failure patients.
Abstract translation: 制备方法。 的胰岛素样生长因子1（IGF-1）（I）通过用表达载体contg转化大肠杆菌菌株。 cDNA编码（I）（从人肝细胞提取和分离的mRNA制备），以与另一基因的融合基因的形式插入cDNA，然后培养和纯化。 所得到的（I）的改进包括利用作为在（I）产生和另一个基因之间具有消旋酶酶识别位点的质粒的表达载体，并且所得融合蛋白用肠激酶切割。 产品 （I）用于控制不能用胰岛素治疗的糖尿病患者的血糖水平，局部治疗骨折和治疗先天性获得性生长衰竭患者。
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5.发明公开
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Patent Agency Ranking
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7.发明授权
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公开(公告)号：KR1019960016716B1
公开(公告)日：1996-12-20
申请号：KR1019930009703
申请日：1993-05-31
Applicant: 한국과학기술연구원
IPC: G01N33/49
Abstract: The measuring method of apolipo-protein concentration in blood comprises the steps of separating lipid-protein complex fractions with a density of below 1.225g/ml obtained by single centrifugation of blood, or lipid-free fractions by treating the lipid protein complex fractions with organic solvent; coating the fractions on a microtiter plate or an appropriate solid surface; adding samples and anti-apolipo protein antibodies into the microtiter plate and reacting; and then measuring the apolipo-protein concentration by general ELISA method.
Abstract translation: 血液中载脂蛋白浓度的测定方法包括以下步骤：通过单次离心血液或无脂质组分，通过用有机物处理脂质蛋白复合物级分，分离密度低于1.225g / ml的脂质 - 蛋白复合物级分 溶剂; 在微量滴定板或适当的固体表面上涂布级分; 将样品和抗Apolipo蛋白质抗体加入到微量滴定板中并进行反应; 然后用普通ELISA法测定载脂蛋白浓度。
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公开(公告)号：KR100185334B1
公开(公告)日：1999-04-01
申请号：KR1019950039459
申请日：1995-11-02
Applicant: 한국과학기술연구원
IPC: C12N15/13
CPC classification number: C07K16/18 , Y10S435/849
Abstract: 본 발명은 인간 혈장 아포지단백질 B-100에 결합하는 생쥐 항체의 항원 결합 절편(Fab) 부위의 중사슬과 경사슬을 암호하는 cDNA들에 관한 것이다.
이는 세포내 발현에 의해 심장 순환기 질환 등의 진단과 관련하여 혈장 아포지단백질 B-100의 농도를 측정할 수 있는 급원물질을 제공하고 또한 심장 순환기 질환 등의 치료에 관련하여 아포지단백질 B-100을 포함하고 있는 동맥경화성 지단백질을 혈중으로부터 선택적으로 제거할 수 있는 급원물질을 제공한다.
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