公开(公告)号：KR100681482B1

公开(公告)日：2007-04-16

申请号：KR1020050102609

申请日：2005-10-28

Applicant: 한국과학기술연구원

Inventor： 오창현 ,  조정혁 ,  이수철

IPC: A61K31/7048 ,  A61K36/00

Abstract: 본 발명은 천수근으로부터 추출한 하르파고시드 엑기스를 유기 염기로 가수분해하여 하르파지드를 생성시킨 후, 강염기성 음이온 교환수지 컬럼 크로마토그래피, 실리카겔 컬럼 크로마토그래피 및 활성탄 컬럼 크로마토그래피로 순차적으로 정제하는 것을 포함하는, 천수근으로부터 하르파지드를 간단하면서도 경제적으로 분리하는 방법에 관한 것이다.
하르파지드, 강염기성 음이온 교환수지 크로마토그래피, 활성탄 컬럼 크로마토그래피.

Abstract translation: 本发明包括纯化的顺序的Har，从cheonsugeun提取到随后与有机碱水解，生成哈尔波叠氮化物，强碱性阴离子交换树脂柱层析，硅胶柱色谱法今后籽提取物和活性炭柱色谱法 从经济角度来看，

公开(公告)号：KR1020000051067A

公开(公告)日：2000-08-16

申请号：KR1019990001305

申请日：1999-01-18

Applicant: 한국과학기술연구원

Inventor： 조정혁 ,  오창현 ,  이기수 ,  이수철

IPC: C07D473/00

Abstract: PURPOSE: A purine compound having a substitutent group at the C-8 position is provided which is expected to be useful as an inhibitor of cyclin-dependent kinase. CONSTITUTION: A purine compound represented by the formula 1 having a substitutent group at the C-8 position is prepared, Thus, urea and ethylisocyanoacetate are subjected to heating reflux in the presence of a base to give a compound of formula 2, which is subjected to heating reflux after nitration and treated with sodium hydrosulfide to give a compound of formula 3, which is subjected to reaction continuously to give the title compound. In formula, R1 is methyl or phenyl; R2 is ethanolamine, or 2-amino-3-methyl-1-butanol.

Abstract translation: 目的：提供在C-8位置具有取代基的嘌呤化合物，其预期可用作细胞周期蛋白依赖性激酶的抑制剂。 构成：制备在C-8位具有取代基的式1表示的嘌呤化合物。因此，在碱的存在下，将脲和乙基异氰酸乙酯进行加热回流，得到式2的化合物， 硝化后加热回流并用氢硫化钠处理，得到式3的化合物，其连续进行反应，得到标题化合物。 在式中，R 1是甲基或苯基; R2是乙醇胺，或2-氨基-3-甲基-1-丁醇。

公开(公告)号：KR1020000050975A

公开(公告)日：2000-08-05

申请号：KR1019990001184

申请日：1999-01-16

Applicant: 한국과학기술연구원

Inventor： 조정혁 ,  오창현 ,  이수철 ,  이기수

IPC: C07D473/00

Abstract: PURPOSE: Provided are purine derivatives useful as an inhibitor of the cycline-dependent kinases(CDK) and a preparation method thereof. The purine derivatives are useful as an anti-cancer agent and an inhibitor of CDK because of their high selectivity to the CDK. CONSTITUTION: A purine derivatives are represented by formula(1), wherein R1 is methyl, isopropyl, allyl, cyclopropyl; and R2 is 4-(2-hydroxyethyl)piperazine, 4-hydroxypiperidine, thiomorpholin, 2-hydroxymethylpyrrolidine. Adenine is used as a starting material to prepare the purine derivatives instead of 2, 6-dichloropurine thereby being easy to handle and cutting down expenses.

Abstract translation: 目的：提供可用作循环依赖性激酶（CDK）的抑制剂的嘌呤衍生物及其制备方法。 嘌呤衍生物由于其对CDK的高选择性而可用作抗癌剂和CDK抑制剂。 构成：嘌呤衍生物由式（1）表示，其中R 1是甲基，异丙基，烯丙基，环丙基; R2是4-（2-羟乙基）哌嗪，4-羟基哌啶，硫代吗啉，2-羟甲基吡咯烷。 腺嘌呤被用作制备嘌呤衍生物而不是2,6-二氯嘌呤的起始原料，因此易于处理和降低费用。