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Patent Agency Ranking
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公开(公告)号：KR1019880002313B1
公开(公告)日：1988-10-22
申请号：KR1019860002080
申请日：1986-03-20
Applicant: 한국과학기술연구원 , 대한민국(농촌진흥청장)
IPC: C12N1/00
Abstract: Root-nodle bacteria inocula for leguminous plants were prepd. Thus, Rhizobium japonicum KLTL 8182p was cultured on the medium contg. 10 mannitol. 1.0 yeast extract, 0.2 MgSO4, 0.2 NaCl, and 0.2g K2HPO4 per liter of water for 3 days at 28↿C. 100 peat, or 100 lignite, or 100 zelite, or 100 g leaf mold was pulverized to > 100 mesh particles, autoclaved for 3 hr at 121↿C,and mixed with the above cultures (40ml), 0.5 g Me, and 5 g B. The mixt. was packed into a polyethylenevinyl bag, sealed, and incubated for 2 weeks at room temp. to provide inocula availible for 1000m2 soils.
Abstract translation: 豆科植物根瘤细菌接种物 因此，日本根瘤菌KLTL 8182p在培养基上培养。 10甘露醇。 1.0酵母提取物，0.2 MgSO 4，0.2 NaCl和0.2g K 2 HPO 4 /升水，在28℃下3天。 将100个泥炭或100个褐煤或100个zelite或100g叶霉粉碎至> 100目的颗粒，在121℃下高压灭菌3小时，并与上述培养物（40ml），0.5g Me和5g 混合 装入聚乙烯乙烯基袋中，密封，并在室温下孵育2周。 提供可用于1000m2土壤的接种物。
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公开(公告)号：KR1019890004023B1
公开(公告)日：1989-10-16
申请号：KR1019870014288
申请日：1987-12-15
Applicant: 한국과학기술연구원
IPC: C12N1/38
Abstract: The method for preparing Rhizobium as inoculum comprises: (a) culturing Rhizobium japonium KCTC in the medium containing 100g of mannitol, 1.0 of yeast extract, 0.2 of MgSO4, 0.2 of NaCl, 0.2 of K2HPO4 and 1 liter distilled water at 28≦̸C for 4 days; (b) pulverizing 100g of perlite and sterilizing at 121 C for 2 hrs; (c) putting the pulverized perlite to the above culture broth in an aseptic condition and mixing; (d) aging the mixture at 25≦̸C for two weeks.
Abstract translation: 用于制备根瘤菌作为接种物的方法包括：（a）在含有100g甘露醇，1.0份酵母提取物，0.2份MgSO 4，0.2份NaCl，0.2份K 2 HPO 4和2升蒸馏水的培养基中培养根瘤菌KCTC，在28＆amp; 4天; （b）粉碎100g珍珠岩并在121℃下灭菌2小时; （c）在无菌条件下将粉碎的珍珠岩放入上述培养液中并混合; （d）将混合物在25℃下老化2周。
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