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    두과근류균 접종제의 제조방법
    2.
    发明授权
    두과근류균 접종제의 제조방법 失效
    生产用于植物的植物材料的方法

    公开(公告)号：KR1019880002313B1

    公开(公告)日：1988-10-22

    申请号：KR1019860002080

    申请日：1986-03-20

    Applicant: 한국과학기술연구원 ,  대한민국(농촌진흥청장)

    Inventor： 민태익 ,  유익동 ,  김창진 ,  김성훈 ,  이윤 ,  김석동 ,  이상규

    IPC: C12N1/00

    Abstract: Root-nodle bacteria inocula for leguminous plants were prepd. Thus, Rhizobium japonicum KLTL 8182p was cultured on the medium contg. 10 mannitol. 1.0 yeast extract, 0.2 MgSO4, 0.2 NaCl, and 0.2g K2HPO4 per liter of water for 3 days at 28↿C. 100 peat, or 100 lignite, or 100 zelite, or 100 g leaf mold was pulverized to > 100 mesh particles, autoclaved for 3 hr at 121↿C,and mixed with the above cultures (40ml), 0.5 g Me, and 5 g B. The mixt. was packed into a polyethylenevinyl bag, sealed, and incubated for 2 weeks at room temp. to provide inocula availible for 1000m2 soils.

    Abstract translation: 豆科植物根瘤细菌接种物 因此，日本根瘤菌KLTL 8182p在培养基上培养。 10甘露醇。 1.0酵母提取物，0.2 MgSO 4，0.2 NaCl和0.2g K 2 HPO 4 /升水，在28℃下3天。 将100个泥炭或100个褐煤或100个zelite或100g叶霉粉碎至> 100目的颗粒，在121℃下高压灭菌3小时，并与上述培养物（40ml），0.5g Me和5g 混合 装入聚乙烯乙烯基袋中，密封，并在室温下孵育2周。 提供可用于1000m2土壤的接种物。

    핵산함유량이 높은 새로운 칸디다 속 효모 칸디다 노베지카
    3.
    发明授权
    핵산함유량이 높은 새로운 칸디다 속 효모 칸디다 노베지카 失效
    具有高含量的核酸假丝酵母的新型酵母菌株

    公开(公告)号：KR100314903B1

    公开(公告)日：2001-11-23

    申请号：KR1019990005821

    申请日：1999-02-22

    Applicant: 한국과학기술연구원

    Inventor： 안종석 ,  박찬선 ,  민태익 ,  이현선 ,  안순철 ,  김보연 ,  강대욱 ,  오원근

    IPC: C12N1/16

    Abstract: 본발명은핵산을다량함유하고있는칸디다속의새로운효모균주인칸디다노베지카 ()에관한것으로, 구체적으로살펴보면본 발명의칸디다속의새로운미생물인칸디다노베지카 CRM-9802 (CRM-9802)는조미성분인 GMP(guanosine monophosphate)와 IMP(inosine monophosphate)로변환시킬수 있는 RNA의함량이높은유용한특징을가지고있으므로, RNA 분해법에의한핵산조미료생산에있어우수한 RNA제공원으로이용될수 있을뿐 아니라효모자체의균체추출물상태로만들어지는천연조미료의제조에효과적으로사용될수 있다.

    신규 락토바실러스 속 (LACTOBACILLUS SP.)MT-1077 및 그로부터 생산되는 신규 박테리오신
    9.
    发明授权
    신규 락토바실러스 속 (LACTOBACILLUS SP.)MT-1077 및 그로부터 생산되는 신규 박테리오신 失效
    신규락토바실러스속（LACTOBACILLUS SP。）MT-1077및从산되는신규박테리신신

    公开(公告)号：KR100351622B1

    公开(公告)日：2004-12-29

    申请号：KR1019980063818

    申请日：1998-12-31

    Applicant: 한국과학기술연구원

    Inventor： 민태익 ,  안종석 ,  이헌주 ,  박찬선 ,  김승호 ,  이현선 ,  주윤정

    IPC: C12N1/20 ,  C12N15/31

    Abstract: PURPOSE: A novel lactobacillus sp. MT-1077 (KCTC 8903P) and a novel bacteriocin produced therefrom are provided. The bacteriocin has stability against broad range of pH, heat stability and antifungal activity and used as food preservation agent, biological fermentation regulator, and antifungal agent. CONSTITUTION: A lactobacillus MT-1077 is isolated from Kimchi. The method for purifying bacteriocin from lactobacillus MT-1077 comprises the steps of: incubating lactobacillus MT-1077 in MRS broth; precipitating the lactobacillus MT-1077 fermented culture broth by addition of ammonium hydrogen sulfate; passing the protein precipitates through the column packed with carboxymethyl sepharose CL-6B and eluting the adsorbed protein with 0.1M sodium chloride; passing the elutes through the column packed with phenyl sepharose CL-4B and eluting the adsorbed protein with 10% ammonium hydrogen sulfate solution; and purifying the elutes by HPLC

    Abstract translation: 目的：一种新型乳酸菌。 提供了MT-1077（KCTC 8903P）和由其生产的新型细菌素。 该细菌素具有广泛的pH，热稳定性和抗真菌活性的稳定性，可用作食品保鲜剂，生物发酵调节剂和抗真菌剂。 构成：从泡菜中分离出乳酸菌MT-1077。 从乳酸菌MT-1077纯化细菌素的方法包括以下步骤：在MRS肉汤中温育乳酸菌MT-1077; 通过加入硫酸氢铵沉淀乳酸菌MT-1077发酵培养液; 使蛋白质沉淀物通过填充有羧甲基琼脂糖凝胶CL-6B的柱，并用0.1M氯化钠洗脱吸附的蛋白质; 将洗脱液通过用苯基琼脂糖凝胶CL-4B填充的柱并用10％硫酸氢铵溶液洗脱吸附的蛋白质; 并通过HPLC纯化洗脱液

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