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公开(公告)号:KR101371361B1
公开(公告)日:2014-03-07
申请号:KR1020120137304
申请日:2012-11-29
Applicant: 한국과학기술연구원
Abstract: The present invention relates to a method for quantifying fatty oil using means IV, and more specifically, to a method for quantifying fatty oil in a living body using means IV. According to one embodiment of the present invention, liquid chromatography (Nanospace SI-2, Shiseido co. Ltd, Tokyo, Japan) with high performance using a sunfire C8 column is used to separate an analyte (triolein, diolein, and monoolein) from an extracted solution. [Reference numerals] (AA) Weight of oil(mg) converted to Sudan IV; (BB) Weight of oil(mg) analyzed to LC/MS; (CC) Sudan recovery rate/Oil recovery rate; (DD) Amount of injected oil(쨉L)
Abstract translation: 本发明涉及使用方法IV定量脂肪油的方法,更具体地,涉及使用方法IV定量生物体中的脂肪油的方法。 根据本发明的一个实施方案,使用具有高性能的Sunfire C8柱的液相色谱(Nanospace SI-2,Shiseido co。Ltd,Tokyo,Japan)将分析物(三油酸甘油酯,二油烯和单油酸酯)从 提取溶液。 (附图标记)(AA)转换为苏丹的油重量(mg)IV; (BB)油液重量(mg)分析至LC / MS; (CC)苏丹回收率/回收率; (DD)注油量(쨉L)