公开(公告)号：KR102095784B1

公开(公告)日：2020-04-01

申请号：KR1020190049045

申请日：2019-04-26

Applicant: 한국과학기술연구원

Inventor： 이연희 ,  이강봉 ,  김선희 ,  장윤정

IPC: G01N27/62 ,  G01N30/72

公开(公告)号：KR1020010047748A

公开(公告)日：2001-06-15

申请号：KR1019990052105

申请日：1999-11-23

Applicant: 한국과학기술연구원

Inventor： 명승운 ,  김명수 ,  장윤정

IPC: G01N33/546

Abstract: PURPOSE: A method for analyzing a homocysteine concentration in blood is provided which is excellent in sensitivity, improvement of reproductivity, accuracy, shortening of analysis time, low cost and conveniency. CONSTITUTION: The method comprises steps of: (i) reacting homocysteine with ethyl chloroformate and 2,2,3,3,3-pentafluoro-1-propanol to prepare a derivative of homocysteine; (ii) extracting the homocysteine derivative with an organic solvent; and (iii) analyzing the clean-up homocysteine derivative by using a gas chromatography-electron capture detector.

Abstract translation: 目的：提供一种分析血液中高半胱氨酸浓度的方法，灵敏度优异，繁殖力好，准确度高，分析时间缩短，成本低廉等优点。 方法：该方法包括以下步骤：（i）使高半胱氨酸与氯甲酸乙酯和2,2,3,3,3-五氟-1-丙醇反应制备同型半胱氨酸的衍生物; （ii）用有机溶剂萃取同型半胱氨酸衍生物; 和（iii）通过使用气相色谱 - 电子捕获检测器分析清除同型半胱氨酸衍生物。

公开(公告)号：KR100330860B1

公开(公告)日：2002-04-03

申请号：KR1019990052105

申请日：1999-11-23

Applicant: 한국과학기술연구원

Inventor： 명승운 ,  김명수 ,  장윤정

IPC: G01N33/546

Abstract: 본발명은호모시스테인의혈중농도를분석하는방법에관한것으로, 상기방법은호모시스테인을에틸클로로포르메이트및 2,2,3,3,3-펜타플루오로-1-프로판올과반응시켜호모시스테인을유도체화한후 그농도를결정하는것을특징으로한다. 상기방법은보다구체적으로는호모시스테인을에틸클로로포르메이트및 2,2,3,3,3-펜타플루오로프로판올과반응시켜호모시스테인을유도체화하고, 이를유기용매를사용하여추출한후, 기체크로마토그래피-전자포획검출기를사용하여호모시스테인의농도를분석하는것으로이루어진다. 본발명의방법에의해호모시스테인의혈중농도를분석할경우, 우수한감도, 분석시간의단축, 저비용및 편리성등을동시에얻을수 있다.