公开(公告)号：KR100687281B1

公开(公告)日：2007-02-27

申请号：KR1020050116037

申请日：2005-11-30

Applicant: 한국과학기술연구원

Inventor： 한동근 ,  안광덕 ,  차승환

IPC: A61K47/48 ,  A61P43/00

Abstract: Injectable thermosensitive pluronic hydrogels coupled with bioactive materials for tissue regeneration and a preparation method thereof are provided to improve cell-affinity of the pluronic hydrogels while their thermosensitivity is maintained, so that they are useful for tissue regeneration. The injectable thermosensitive pluronic hydrogels coupled with bioactive materials through meta-acryloxyethyl trimellitic acid for tissue regeneration, represented by the formula(1) is provided, wherein R is a physiologically activating material; the metacryloxyethyl trimellitic acid is 4-metacryloxyethyl trimellitic acid or 2-methacryloxyethyl trimellitic acid; and the physiologically activating material is a cell-affinity ligand peptide or growth factor. The method for preparing the injectable thermosensitive pluronic hydrogels coupled with bioactive materials through meta-acryloxyethyl trimellitic acid for tissue regeneration comprises the steps of: (1) reacting the thermosensitive pluronic polymer with metacryloxyethyl trimellitic acid anhydride at room temperature to prepare the metacryloxyethyl trimellitic acid-fluronic polymer; and (2) coupling the metacryloxyethyl trimellitic acid-fluronic polymer with a cell-affinity ligand peptide or growth factor.

Abstract translation: 提供与用于组织再生的生物活性材料偶联的可注射热敏性普朗尼克水凝胶及其制备方法，以改善普朗尼克水凝胶的细胞亲和力，同时保持其热敏性，从而它们可用于组织再生。 提供了由式（1）表示的通过用于组织再生的偏丙烯酰氧基乙基偏苯三酸与生物活性材料偶联的可注射热敏性普朗尼克水凝胶，其中R为生理活性物质; 甲基丙烯酰氧基乙基偏苯三酸为4-甲基丙烯酰氧基乙基偏苯三酸或2-甲基丙烯酰氧基乙基偏苯三酸; 并且生理活化物质是细胞亲和配体肽或生长因子。 通过间 - 丙烯酰氧基乙基偏苯三酸与生物活性材料偶联制备用于组织再生的可注射热敏性普朗尼克水凝胶的方法包括以下步骤：（1）在室温下使热敏聚醚聚合物与甲基丙烯酰氧基乙基偏苯三酸酐反应以制备甲基丙烯酰氧基乙基偏苯三酸 - 氟聚合物; 和（2）将甲基丙烯酰氧乙基偏苯三酸 - 氟隆聚合物与细胞亲和配体肽或生长因子偶联。