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1.发明授权인체 리포코틴을 E. coli에서 생산시 가용성인 상태로 생산 정제하는 방법 및 인체 리포코틴 cDNA 서열을 함유하는 벡터 失效当在大肠杆菌中生产时，以可溶状态生产和纯化人脂皮质蛋白的方法和生产含有人脂皮质蛋白cDNA序列的载体的方法
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3.发明授权
公开(公告)号：KR1019910002855B1
公开(公告)日：1991-05-06
申请号：KR1019890000223
申请日：1989-01-11
Applicant: 한국과학기술연구원
IPC: C12N15/00
Abstract: A process for production of plasmid pLCI comprises (a) isolating mRNA from human placentatissue, (b) preparing 2 chains of cDNA from the obtd. mRNA, (c) attaching (dC)n tail to the cDNA and subjecting it to recombination with plasmid vector, (d) transforimg E. coli with the recombinant plasmid, and (e) selecting clones having human lipocortin I coding gene in the transformant E. coli. Using the plasmid pLCI, human lipocortin I can be produced in soluble form. Lipocortin is useful as an anti-inflammatory protein.
Abstract translation: 生产质粒pLCI的方法包括（a）从人胎盘组织中分离mRNA，（b）从该实验室制备2条cDNA链。 mRNA，（c）将（dC）n尾连接到cDNA上并使其与质粒载体重组，（d）用重组质粒转导大肠杆菌，和（e）在转化体中选择具有人脂蛋白I编码基因的克隆 大肠杆菌 使用质粒pLCI，人脂蛋白I可以以可溶形式生产。 脂皮素可用作抗炎蛋白。
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