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1.发明授权
公开(公告)号：KR1019910002855B1
公开(公告)日：1991-05-06
申请号：KR1019890000223
申请日：1989-01-11
Applicant: 한국과학기술연구원
IPC: C12N15/00
Abstract: A process for production of plasmid pLCI comprises (a) isolating mRNA from human placentatissue, (b) preparing 2 chains of cDNA from the obtd. mRNA, (c) attaching (dC)n tail to the cDNA and subjecting it to recombination with plasmid vector, (d) transforimg E. coli with the recombinant plasmid, and (e) selecting clones having human lipocortin I coding gene in the transformant E. coli. Using the plasmid pLCI, human lipocortin I can be produced in soluble form. Lipocortin is useful as an anti-inflammatory protein.
Abstract translation: 生产质粒pLCI的方法包括（a）从人胎盘组织中分离mRNA，（b）从该实验室制备2条cDNA链。 mRNA，（c）将（dC）n尾连接到cDNA上并使其与质粒载体重组，（d）用重组质粒转导大肠杆菌，和（e）在转化体中选择具有人脂蛋白I编码基因的克隆 大肠杆菌 使用质粒pLCI，人脂蛋白I可以以可溶形式生产。 脂皮素可用作抗炎蛋白。
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Patent Agency Ranking
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4.发明公开신규한 플라스미드 제조방법 및 그를 함유한 균주를 배양하여 인터루킨-2 또는 변이 인터루킨-2를 생산하는 방법 失效培养含有其的菌株的新型质粒生产方法和生产白细胞介素-2或突变型白细胞介素-2的方法
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6.发明授权인체 리포코틴을 E. coli에서 생산시 가용성인 상태로 생산 정제하는 방법 및 인체 리포코틴 cDNA 서열을 함유하는 벡터 失效当在大肠杆菌中生产时，以可溶状态生产和纯化人脂皮质蛋白的方法和生产含有人脂皮质蛋白cDNA序列的载体的方法
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公开(公告)号：KR1019890003715B1
公开(公告)日：1989-09-30
申请号：KR1019870006560
申请日：1987-06-27
Applicant: 한국과학기술연구원
IPC: C12N15/00
Abstract: A fragment having -300 base pairs was obtained from a plasmid, PILM 7, cut by XbaI and PvuII. On the other hand, a fragment having - 4,500 base pairs was obtained from aplasmid, pNK2, treated with XbaI and PvuII. Both of the fragments were ligated and inserted into a proper strain to get pNKM 21. pNKM 21 was inserted into M 5248 strain to get KCTC 8258 P strain followed by culturing it to give a interleukin-2 contg. serine at 125-position instead of cystein.
Abstract translation: 从由XbaI和PvuII切割的质粒PILM7获得具有-300个碱基对的片段。 另一方面，从用XbaI和PvuII处理的质粒pNK2获得具有-4,500个碱基对的片段。 将两个片段连接并插入适当的菌株中以获得pNKM21。将pNKM 21插入M 5248菌株中以获得KCTC 8258P菌株，然后培养以产生白细胞介素-2对照。 丝氨酸位于125位，而不是半胱氨酸。
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