-
公开(公告)号：KR1019960034402A
公开(公告)日：1996-10-22
申请号：KR1019950007251
申请日：1995-03-31
Applicant: 한국과학기술원
Abstract: 본 발명은 스트렙토키나제를 발현하는 미생물 및 그를 이용한 스트렙토키나제의 제조방법에 관한 것이다. 좀 더 구체적으로, 본 발명은 스트렙토키나제 발현벡터와 그것으로 형질전환된 재조합 미생물 및 재조합 미생물로부터 스트렙토키나제를 제조하는 방법에 관한 것이다. 본 발명의 스트렙토키나제 발현벡터 pSK100으로 형질전환된 재조합 미생물을 배양하는 경우, 임상적으로 안전한 고순도의 스트렙토키나제를 경제적으로 대량 생산하는 효과를 가진다.
-
公开(公告)号：KR100134997B1
公开(公告)日：1998-04-20
申请号：KR1019950007251
申请日：1995-03-31
Applicant: 한국과학기술원
Abstract: 본 발명은 스트렙토키나제를 발현하는 미생물 및 그를 이용한 스트렙토키나제의 제조방법에 관한 것이다. 좀 더 구체적으로, 본 발명은 스트렙토키나제 발현벡터와 그것으로 형질전환된 재조합 미생물 및 재조합 미생물로부터 스트렙토키나제를 제조하는 방법에 관한 것이다. 본발명의 스트렙토키나제 발현벡터 pSK100으로 형질전환된 재조합 미생물을 배양하는 경우, 임상적으로 안전한 고순도의 스트렙토키나제를 경제적으로 대량생산하는 효과를 가진다.
-
公开(公告)号：KR100134835B1
公开(公告)日：1998-04-14
申请号：KR1019950003325
申请日：1995-02-21
Applicant: 한국과학기술원
IPC: C12N15/57
Abstract: A subtilisin J gene of Bacillus stearothermophilus is treated with restriction enzyme Hind II to give DNA segment, introduced to pUC19 and deformed by the mutation-inducing oligonucleotide to obtain the reverse-polymerization chain starting material, followed by polymerization chain reaction(PCR) to give 982bp DNA band which is the first PCR product. The second PCR is performed by adding 17bp forward primer to the 982 bp DNA to obtain precipitate, which is dissolved and treated with restriction enzyme Hind II to give aspartic acid-substituted subtilIsin J.
Abstract translation: 用限制性内切酶Hind II处理嗜热脂肪芽孢杆菌的枯草杆菌蛋白酶J基因，得到导入pUC19并通过突变诱导寡核苷酸变形的DNA片段，得到反向链原料，随后进行聚合反应（PCR），得到 982bp DNA条带是第一个PCR产物。 通过向982bp DNA中加入17bp正向引物进行第二次PCR，得到沉淀，将其溶解并用限制酶Hind II处理，得到天冬氨酸取代的subtilIsin J.
-
Patent Agency Ranking
公开(公告)号：KR100411238B1
公开(公告)日：2003-12-18
申请号：KR1020000048124
申请日：2000-08-19
Applicant: 한국과학기술원
IPC: C12N1/20
Abstract: PURPOSE: Provided is a novel Bacillus sp. whose wprA gene encoding WprA, proteinase of the Bacillus subtilis, is destroyed by homologous recombination to stably express foreign proteins without damaging them. The foreign proteins are produced by transforming the strains with an expression vector and cultured. CONSTITUTION: The preparation method of the Bacillus sp. strain comprises the steps of: constructing gene replacement vector containing wprA gene, Bacillus origin and E. coli origin in which resistant genes to ampicillin and tetracycline are inserted; transforming Bacillus subtilis WB600 using the vector; selecting tetracycline resistant strains and heat-treating them to eliminate the unincorporated vector; screening the genomic DNA of the selected strains by polymerase chain reaction and confirming that wrpA gene is destructed from the genomic DNA of the selected strains; and identifying the strain Bacillus subtilis LB700 (KCTC 18039P) and using it as a host strain to express staphylokinase in a large amount.
Abstract translation: 目的：提供了一种新型芽孢杆菌属。 其编码枯草芽孢杆菌蛋白酶WprA的wprA基因被同源重组破坏，从而稳定表达外源蛋白而不损害它们。 通过用表达载体转化菌株并培养来产生外源蛋白质。 组成：芽孢杆菌的制备方法。 菌株包括以下步骤：构建含有wprA基因，芽孢杆菌来源和大肠杆菌来源的基因置换载体，其中插入对氨苄青霉素和四环素的抗性基因; 使用该载体转化枯草芽孢杆菌WB600; 选择四环素耐药菌株并对它们进行热处理以消除未结合的载体; 通过聚合酶链式反应筛选所选菌株的基因组DNA，并证实wrpA基因从所选菌株的基因组DNA中被破坏; 并鉴定菌株枯草芽孢杆菌LB700（KCTC 18039P）并将其用作宿主菌株以大量表达葡萄球菌激酶。
-
公开(公告)号：KR1020020014923A
公开(公告)日：2002-02-27
申请号：KR1020000048124
申请日：2000-08-19
Applicant: 한국과학기술원
IPC: C12N1/20
Abstract: PURPOSE: Provided is a novel Bacillus sp. whose wprA gene encoding WprA, proteinase of the Bacillus subtilis, is destroyed by homologous recombination to stably express foreign proteins without damaging them. The foreign proteins are produced by transforming the strains with an expression vector and cultured. CONSTITUTION: The preparation method of the Bacillus sp. strain comprises the steps of: constructing gene replacement vector containing wprA gene, Bacillus origin and E. coli origin in which resistant genes to ampicillin and tetracycline are inserted; transforming Bacillus subtilis WB600 using the vector; selecting tetracycline resistant strains and heat-treating them to eliminate the unincorporated vector; screening the genomic DNA of the selected strains by polymerase chain reaction and confirming that wrpA gene is destructed from the genomic DNA of the selected strains; and identifying the strain Bacillus subtilis LB700 (KCTC 18039P) and using it as a host strain to express staphylokinase in a large amount.
Abstract translation: 目的：提供一种新型芽孢杆菌 其编码WprA的wprA基因，枯草芽孢杆菌的蛋白酶被同源重组破坏以稳定表达外源蛋白质而不损害它们。 通过用表达载体转化菌株并培养来产生外源蛋白质。 构成：芽孢杆菌的制备方法 菌株包括以下步骤：构建含有wprA基因，芽孢杆菌来源和大肠杆菌来源的基因置换载体，其中插入氨苄青霉素和四环素的抗性基因; 使用载体转化枯草芽孢杆菌WB600; 选择四环素抗性菌株并进行热处理以消除未掺入的载体; 通过聚合酶链反应筛选所选菌株的基因组DNA，并证实wrpA基因从所选菌株的基因组DNA中破坏; 并鉴定菌株枯草芽孢杆菌LB700（KCTC 18039P），并将其用作宿主菌株以大量表达葡萄球菌激酶。
-
6.发明授权세포접착 펩타이드 씨에이피 유전자를 포함하는재조합벡터, 형질전환된 대장균, 및 반복연결된씨에이피의 제조방법 失效세포접착펩타이드씨에이피유전자를포함하는재조합벡터，형질전환된대장균，및반복연결된씨에이피의제조방법
公开(公告)号：KR100381847B1
公开(公告)日：2003-04-26
申请号：KR1020000010769
申请日：2000-03-03
Applicant: 한국과학기술원
IPC: C12N15/63
Abstract: PURPOSE: Provided is a recombinant vector which comprises repeat-linked cell adhesion peptide(R-CAP) genes, thereby accumulating the R-CAP in periplasm of cell so that R-CAP can be easily purified. And an E.coli transformant and a method for preparing R-CAP therefrom are also provided. CONSTITUTION: The recombinant vector is constructed by cloning a gene for encoding CAP into a vector having a sequence encoding maltose binding protein(MBP). The cloning is preferably repeated 1 to 15 times. The recombinant vector preferably contains tac promoter. The vector is selected from pMal-p2, pMal-c2 and the like, and E.coli strain is selected from E.coli JM109, E.coli DH5α, or E.coli SF130. The method of preparing R-CAP comprises the steps of culturing an E.coli transformant and recovering the R-CAP from periplasm fraction. The recovered R-CAP is polypeptide that a CAP unit is repeat-linked 1 to 30 times. The R-CAP is added to pharmaceutical compositions for treating the wound and for alleviating the stimulation of skin. R-CAP can be used in an amount of 0.1 to 10 microgram/day.
Abstract translation: 目的：提供一种包含重复连接的细胞粘附肽（R-CAP）基因的重组载体，由此在细胞的周质中积聚R-CAP，从而可以容易地纯化R-CAP。 还提供了大肠杆菌转化体和由其制备R-CAP的方法。 组成：通过将编码CAP的基因克隆到具有编码麦芽糖结合蛋白（MBP）的序列的载体中来构建重组载体。 克隆优选重复1至15次。 重组载体优选含有tac启动子。 载体选自pMal-p2，pMal-c2等，大肠杆菌菌株选自大肠杆菌JM109，大肠杆菌DH5α或大肠杆菌SF130。 制备R-CAP的方法包括培养大肠杆菌转化体和从周质部分回收R-CAP的步骤。 回收的R-CAP是CAP单元重复连接1至30次的多肽。 将R-CAP添加到用于治疗伤口和减轻皮肤刺激的药物组合物中。 R-CAP可以以0.1至10微克/天的量使用。
-
7.发明公开세포접착 펩타이드 씨에이피 유전자를 포함하는재조합벡터, 형질전환된 대장균, 및 반복연결된씨에이피의 제조방법 失效包含细胞粘附肽（CAP）基因的重组载体，其转化的E.COLI及其制备重复连接的CAP的方法
公开(公告)号：KR1020010086847A
公开(公告)日：2001-09-15
申请号：KR1020000010769
申请日：2000-03-03
Applicant: 한국과학기술원
IPC: C12N15/63
Abstract: PURPOSE: Provided is a recombinant vector which comprises repeat-linked cell adhesion peptide(R-CAP) genes, thereby accumulating the R-CAP in periplasm of cell so that R-CAP can be easily purified. And an E.coli transformant and a method for preparing R-CAP therefrom are also provided. CONSTITUTION: The recombinant vector is constructed by cloning a gene for encoding CAP into a vector having a sequence encoding maltose binding protein(MBP). The cloning is preferably repeated 1 to 15 times. The recombinant vector preferably contains tac promoter. The vector is selected from pMal-p2, pMal-c2 and the like, and E.coli strain is selected from E.coli JM109, E.coli DH5α, or E.coli SF130. The method of preparing R-CAP comprises the steps of culturing an E.coli transformant and recovering the R-CAP from periplasm fraction. The recovered R-CAP is polypeptide that a CAP unit is repeat-linked 1 to 30 times. The R-CAP is added to pharmaceutical compositions for treating the wound and for alleviating the stimulation of skin. R-CAP can be used in an amount of 0.1 to 10 microgram/day.
Abstract translation: 目的：提供重复载体，其包含重复连接的细胞粘附肽（R-CAP）基因，从而在细胞周质中积累R-CAP，从而可以容易地纯化R-CAP。 还提供了大肠杆菌转化体及其制备R-CAP的方法。 构成：通过将编码CAP的基因克隆到具有编码麦芽糖结合蛋白（MBP）的序列的载体中构建重组载体。 克隆优选重复1至15次。 重组载体优选含有tac启动子。 载体选自pMal-p2，pMal-c2等，大肠杆菌菌株选自大肠杆菌JM109，大肠杆菌DH5α或大肠杆菌SF130。 制备R-CAP的方法包括培养大肠杆菌转化体并从周质级分回收R-CAP的步骤。 回收的R-CAP是CAP单元重复连接1至30次的多肽。 将R-CAP加入用于治疗伤口并减轻皮肤刺激的药物组合物中。 R-CAP的使用量可以为0.1〜10微克/日。
-
公开(公告)号：KR1019960031614A
公开(公告)日：1996-09-17
申请号：KR1019950003325
申请日：1995-02-21
Applicant: 한국과학기술원
IPC: C12N15/57
Abstract: 본 발명은 단백질공확기법을 이용하여 서브틸리신 J의 유전자상에서 염기서열을 변형시킴으로써, 열안정성이 야생형보다 더욱 증진된 돌연변이체 서브틸리신 J 및 그를 제조하는 방법에 관한 것이다. 본 발명에서 제조된 서브틸리신 J의 49번 세린이 아스파라긴산으로 치환되고, 다시 58번 티로신이 결손된 이중 돌연변이체 서브틸리신 J는 고온에서의 자가분해 방지가 야생형보다 더욱 증진된 단백질 가수분해효소로서, 세제 및 식품 등의 여러 산업적 분야에 이용될 수 있을 것이다.
-
-
-
-
-
-
-
Search Results
8
records
(took 0.016 seconds)
