公开(公告)号：KR100381847B1

公开(公告)日：2003-04-26

申请号：KR1020000010769

申请日：2000-03-03

Applicant: 한국과학기술원

Inventor： 변시명 ,  박지혜 ,  정학수 ,  성득용 ,  배광희 ,  이상준

IPC: C12N15/63

Abstract: PURPOSE: Provided is a recombinant vector which comprises repeat-linked cell adhesion peptide(R-CAP) genes, thereby accumulating the R-CAP in periplasm of cell so that R-CAP can be easily purified. And an E.coli transformant and a method for preparing R-CAP therefrom are also provided. CONSTITUTION: The recombinant vector is constructed by cloning a gene for encoding CAP into a vector having a sequence encoding maltose binding protein(MBP). The cloning is preferably repeated 1 to 15 times. The recombinant vector preferably contains tac promoter. The vector is selected from pMal-p2, pMal-c2 and the like, and E.coli strain is selected from E.coli JM109, E.coli DH5α, or E.coli SF130. The method of preparing R-CAP comprises the steps of culturing an E.coli transformant and recovering the R-CAP from periplasm fraction. The recovered R-CAP is polypeptide that a CAP unit is repeat-linked 1 to 30 times. The R-CAP is added to pharmaceutical compositions for treating the wound and for alleviating the stimulation of skin. R-CAP can be used in an amount of 0.1 to 10 microgram/day.

Abstract translation: 目的：提供一种包含重复连接的细胞粘附肽（R-CAP）基因的重组载体，由此在细胞的周质中积聚R-CAP，从而可以容易地纯化R-CAP。 还提供了大肠杆菌转化体和由其制备R-CAP的方法。 组成：通过将编码CAP的基因克隆到具有编码麦芽糖结合蛋白（MBP）的序列的载体中来构建重组载体。 克隆优选重复1至15次。 重组载体优选含有tac启动子。 载体选自pMal-p2，pMal-c2等，大肠杆菌菌株选自大肠杆菌JM109，大肠杆菌DH5α或大肠杆菌SF130。 制备R-CAP的方法包括培养大肠杆菌转化体和从周质部分回收R-CAP的步骤。 回收的R-CAP是CAP单元重复连接1至30次的多肽。 将R-CAP添加到用于治疗伤口和减轻皮肤刺激的药物组合物中。 R-CAP可以以0.1至10微克/天的量使用。

公开(公告)号：KR1020010086847A

公开(公告)日：2001-09-15

申请号：KR1020000010769

申请日：2000-03-03

Applicant: 한국과학기술원

Inventor： 변시명 ,  박지혜 ,  정학수 ,  성득용 ,  배광희 ,  이상준

IPC: C12N15/63

CPC classification number: C12N15/70 ,  A61K38/00

Abstract: PURPOSE: Provided is a recombinant vector which comprises repeat-linked cell adhesion peptide(R-CAP) genes, thereby accumulating the R-CAP in periplasm of cell so that R-CAP can be easily purified. And an E.coli transformant and a method for preparing R-CAP therefrom are also provided. CONSTITUTION: The recombinant vector is constructed by cloning a gene for encoding CAP into a vector having a sequence encoding maltose binding protein(MBP). The cloning is preferably repeated 1 to 15 times. The recombinant vector preferably contains tac promoter. The vector is selected from pMal-p2, pMal-c2 and the like, and E.coli strain is selected from E.coli JM109, E.coli DH5α, or E.coli SF130. The method of preparing R-CAP comprises the steps of culturing an E.coli transformant and recovering the R-CAP from periplasm fraction. The recovered R-CAP is polypeptide that a CAP unit is repeat-linked 1 to 30 times. The R-CAP is added to pharmaceutical compositions for treating the wound and for alleviating the stimulation of skin. R-CAP can be used in an amount of 0.1 to 10 microgram/day.

Abstract translation: 目的：提供重复载体，其包含重复连接的细胞粘附肽（R-CAP）基因，从而在细胞周质中积累R-CAP，从而可以容易地纯化R-CAP。 还提供了大肠杆菌转化体及其制备R-CAP的方法。 构成：通过将编码CAP的基因克隆到具有编码麦芽糖结合蛋白（MBP）的序列的载体中构建重组载体。 克隆优选重复1至15次。 重组载体优选含有tac启动子。 载体选自pMal-p2，pMal-c2等，大肠杆菌菌株选自大肠杆菌JM109，大肠杆菌DH5α或大肠杆菌SF130。 制备R-CAP的方法包括培养大肠杆菌转化体并从周质级分回收R-CAP的步骤。 回收的R-CAP是CAP单元重复连接1至30次的多肽。 将R-CAP加入用于治疗伤口并减轻皮肤刺激的药物组合物中。 R-CAP的使用量可以为0.1〜10微克/日。