公开(公告)号：KR100948589B1

公开(公告)日：2010-03-23

申请号：KR1020080020343

申请日：2008-03-05

Applicant: 한국과학기술원

Inventor： 김학성 ,  김영필 ,  안장혁

IPC: G01N30/72

Abstract: 본 발명은 저농도 마이코톡신(mycotoxin)의 측정에 관한 것으로서, 보다 상세하게는 다양한 식품 속에 저농도로 존재하는 마이코톡신을 분리 및 정제한 후 금속기판위에 흡착시켜 이차이온 질량분석기로 마이코톡신을 분석하는 방법에 관한 것이다.
본 발명에 의하여 식품 내에 존재하는 인체 내 유해 독성물질을 사전에 빠르고 정확하게 스크리닝할 수 있게 됨으로써 보건의료의 개선 및 독성물질의 연구에 크게 기여할 수 있을 것이다.
마이코톡신, 아플라톡신, 분리, 정제, 이차이온 질량분석, 식품, 독성

公开(公告)号：KR100815259B1

公开(公告)日：2008-03-19

申请号：KR1020060129340

申请日：2006-12-18

Applicant: 남양유업 주식회사 ,  한국과학기술원 ,  (주)에스.앤.디

Inventor： 장치훈 ,  안장혁 ,  최영기 ,  김학성 ,  여경목

IPC: A23D7/04

CPC classification number: A23D7/04

Abstract: A method of manufacturing microencapsulated oil powder using herbal extract and an emulsification buffer agent having antioxidant effect to inhibit oil oxidation and an excipient such as dextrin to coat oil powder is provided to maximally inhibit the formation of oxidation products such as peroxides, aldehydes and ketones. Microencapsulated oil powder having long-lasting freshness is prepared by the step of: mixing 6.3 to 7.3% by weight of milk protein, 22.6 to 23.7% by weight of edible oil and fat, 0.3 to 0.9% by weight of an emulsifier and 68.1 to 70.8% by weight of purified water; adding a mixture of herbal extract and emulsification buffer agent in a weight ratio of 1:0.3 to 0.5 thereto; adding dextrin thereto in a weight ratio of 1:0.3 to 0.5; sterilizing the mixture at 75 to 85deg.C for 20 to 40min; homogenizing the mixture at 3,000 to 3,800rpm for 20 to 40min; filtering with a magnetic container having a filter net of 20 to 60 meshes and a magnetic rod with 8,000 to 12,000 Gauss; and drying with a spray drier. The herbal extract is any one of rosemary extract, broccoli extract and citrus extract. The emulsification buffer agent is monobasic phosphate monobasic, dibasic sodium phosphate, tribasic sodium phosphate, sodium pyrophosphate, monobasic potassium phosphate, dibasic potassium phosphate and tribasic potassium phosphate.

Abstract translation: 提供使用草药提取物制备微胶囊化油粉的方法和具有抑制油氧化的抗氧化作用的乳化缓冲剂和赋予涂料油糊的糊精等赋形剂以最大限度地抑制氧化产物如过氧化物，醛和酮的形成。 通过以下步骤制备具有持久新鲜度的微胶囊化油粉：将6.3至7.3重量％的乳蛋白，22.6至23.7重量％的食用油和脂肪，0.3至0.9重量％的乳化剂和68.1至 70.8重量％的净化水; 以1:0.3至0.5的重量比加入草药提取物和乳化缓冲剂的混合物; 以1:0.3至0.5的重量比加入糊精; 将混合物在75〜85℃下灭菌20〜40分钟; 将混合物在3,000至3800rpm下匀化20至40分钟; 用具有20至60目筛网的磁性容器和8,000至12,000高斯的磁棒过滤; 并用喷雾干燥器干燥。 草药提取物是迷迭香提取物，西兰花提取物和柑橘提取物中的任何一种。 乳化缓冲剂是磷酸二氢钙，磷酸氢二钠，磷酸三钠，焦磷酸钠，磷酸氢二钾，磷酸氢二钾，磷酸三钾等。

公开(公告)号：KR1020090095177A

公开(公告)日：2009-09-09

申请号：KR1020080020343

申请日：2008-03-05

Applicant: 한국과학기술원

Inventor： 김학성 ,  김영필 ,  안장혁

IPC: G01N30/72

Abstract: A method of measuring the mycotoxin of low concentration is provided to analyze the mycotoxin of low concentration easily, precisely and quantitatively by the secondary ion mass spectrometry. A method of measuring the mycotoxin of low concentration comprises the steps of extracting mycotoxin from a target material to separate and purify it; adsorbing the separated and purified mycotoxin on a metal substrate; and analyzing the mycotoxin adsorbed on the metal substrate by using a secondary ion mass spectrometer. The mycotoxin is at least one selected from the group consisting of aflatoxin, ochratoxin, sterigmatocystin, patulin, rubratoxin, fusariumtoxin, zearalenone and deoxynivalenol.

Abstract translation: 提供了一种测定低浓度霉菌毒素的方法，通过二次离子质谱法容易，准确，定量地分析低浓度的真菌毒素。 测定低浓度真菌毒素的方法包括从目标物质提取真菌毒素以分离和纯化其步骤; 将分离纯化的真菌毒素吸附在金属基材上; 并使用二次离子质谱仪分析吸附在金属底物上的霉菌毒素。 真菌毒素是选自黄曲霉毒素，赭曲霉毒素，消菌菌素，patulin，鲁维菌素毒素，镰刀菌毒素，玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇中的至少一种。