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公开(公告)号：KR1020120114008A
公开(公告)日：2012-10-16
申请号：KR1020110031737
申请日：2011-04-06
Applicant: 한국과학기술원
Abstract: PURPOSE: A wastewater refining method using polydopamine is provided to easily regenerate polydopamine silica beads and to remove both organic materials and inorganic materials from wastewater. CONSTITUTION: A wastewater refining method using polydopamine includes the following: polydopamine is coated on a substrate; and the substrate is introduced into wastewater to absorb pollutants. The substrate is selected from a group including silica beads, non-oxidation metals, metal oxides, alkali metals, synthetic polymers, ceramics, and semiconductors. The pollutants are selected from a group including heavy metals, toxic organic materials, and radioactive isotopes. The heavy metals are selected from a group including Cr, Pb, Cd, Hg, Cu, Zn, Co, Ni, Mg, Fe, Mn, and Ca. The toxic organic materials are selected from a group including 4-aminopyridine, 4-methylbenzenethiol, amitrole, starlicide, dicloran, aminopyralid, butylamine, proxan, quinoclamine, thiosemicarbazide, fluoroacetamide, tioclorim, and carbathion.
Abstract translation: 目的：提供使用聚多巴胺的废水精炼方法，以便容易地再生聚多巴胺二氧化硅珠，并从废水中除去有机材料和无机材料。 构成：使用聚多巴胺的废水精炼方法包括以下：将聚多巴胺涂覆在基材上; 并将底物引入废水中以吸收污染物。 基材选自包括二氧化硅珠，非氧化金属，金属氧化物，碱金属，合成聚合物，陶瓷和半导体的组。 污染物从重金属，有毒有机物质和放射性同位素组成。 重金属选自Cr，Pb，Cd，Hg，Cu，Zn，Co，Ni，Mg，Fe，Mn和Ca。 有毒有机材料选自4-氨基吡啶，4-甲基苯硫酚，阿米洛尔，星形化合物，二氯替苯胺，氨基吡啶，丁胺，丙肟，喹唑胺，氨基硫脲，氟乙酰胺，噻氯铵和硫辛酸。
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公开(公告)号：KR100428998B1
公开(公告)日：2004-04-28
申请号：KR1020010055394
申请日：2001-09-10
Applicant: 한국과학기술원
IPC: C07K1/00
CPC classification number: C12N15/1058 , C07K14/43595 , C12N9/86 , C12N15/102
Abstract: The present invention relates to a method for manufacturing a mutant library of proteins with various sizes and sequences from a parental protein, microorganisms transformed with plasmids containing recombinant DNAs prepared by the insertion of a genomic DNA fragment into a defective template, a process for preparing proteins with different sizes and sequences from the parental protein which comprises the steps of culturing the transformed microorganisms and obtaining desired proteins from the culture, and proteins prepared by the said process. In accordance with the invention, a mutant library of proteins with various sizes and sequences can be manufactured from a parental protein in an efficient and simple manner, by constructing a library of microorganisms transformed with recombinant plasmids containing E. coli genomic DNA fragments inserted into defective genes and selecting clones expressing proteins with restored functions or modified characteristics.
Abstract translation: 本发明涉及用于从亲代蛋白质制备具有各种大小和序列的蛋白质突变体文库的方法，用包含通过将基因组DNA片段插入缺陷模板中制备的重组DNA的质粒转化的微生物，制备蛋白质的方法 具有来自亲本蛋白质的不同大小和序列，其包括培养转化的微生物并从培养物获得期望的蛋白质的步骤，以及通过所述方法制备的蛋白质。 根据本发明，通过构建用含有插入缺陷中的大肠杆菌基因组DNA片段的重组质粒转化的微生物文库，可以以有效且简单的方式从亲本蛋白质制备具有各种大小和序列的蛋白质突变体文库 基因并选择表达具有恢复功能或修饰特征的蛋白质的克隆。
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Patent Agency Ranking
公开(公告)号：KR1020030022447A
公开(公告)日：2003-03-17
申请号：KR1020010055394
申请日：2001-09-10
Applicant: 한국과학기술원
IPC: C07K1/00
CPC classification number: C12N15/1058 , C07K14/43595 , C12N9/86 , C12N15/102
Abstract: PURPOSE: A method for manufacturing mutant library of proteins with different sequence and size from a mother protein is provided, thereby the mutant library of proteins can be useful for biotechnology. CONSTITUTION: The method for manufacturing mutant library of proteins with different sequence and size from the mother protein comprises the steps of: (i) inducing mutation to a gene encoding the mother protein to construct a defective template of which function is lost; (ii) inserting a randomly synthesized oligonucleotide sequence or a restriction enzyme-treated genomic DNA into the defective template to construct a recombinant plasmid, and transforming microorganism with the recombinant plasmid to prepare a transformed microorganism library; and (iii) selecting salvage protein of which distinct function is recovered or characteristic is changed from the proteins expressed from the transformed microorganism library, wherein the mutation is induced by deletion or frame shift of nucleotides.
Abstract translation: 目的：提供从母体蛋白质制备具有不同序列和大小的蛋白质突变文库的方法，因此蛋白质突变文库可用于生物技术。 构成：从母体蛋白质制备具有不同序列和大小的蛋白质的突变型文库的方法包括以下步骤：（i）诱导编码母体蛋白质的基因的突变以构建失去功能的缺陷模板; （ii）将随机合成的寡核苷酸序列或限制酶处理的基因组DNA插入缺陷模板中以构建重组质粒，并用重组质粒转化微生物以制备转化的微生物文库; 和（iii）从转化的微生物文库表达的蛋白质中选择回收不同功能或特征的补救蛋白，其中通过核苷酸的缺失或帧移位诱导突变。
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公开(公告)号：KR101875809B1
公开(公告)日：2018-07-06
申请号：KR1020170037503
申请日：2017-03-24
CPC classification number: A61K47/64 , A61K38/00 , A61K47/66 , A61K47/6929 , C07K14/00 , C07K2319/00 , C07K2319/01 , C12N15/62
Abstract: 본발명은리피바디(repebody)를이용한항암단백질약물복합체및 그제조방법에관한것으로, 상세하게는알파나선형캡핑모티프(capping motif)를가지는 LRR(leucine-rich repeat) 패밀리단백질의 N-말단에 VLR(variable lymphocyte receptor)에서기인한 LRR 구조가가변부위로연결되어기질특이성을나타내는리피바디에자기조립되고, 항암활성을가지는단백질약물이접합된리피바디-항암단백질약물복합체(Repebody-anticancer protein drug conjugate)와이를제조하는방법및 그용도에관한것이다. 본발명에따른리피바디-항암단백질약물복합체는리피바디가제공하는높은기질특이성과우수한조직및 종양침투력과, 자기조립되고, 항암활성을가지는단백질약물이결합한것으로써, 리피바디단독으로사용하는경우에비해월등한효능을지니고, 항암단백질약물단독으로사용하는경우에비해특이적선택성을지니는장점을가지고있으며, 이를활용하여암세포에과발현되는단백질에특이적으로결합하는리피바디에, 세포를사멸시키는항암단백질약물을결합시켜암세포만을선택적으로사멸시킴으로써종양치료에유용하다.
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公开(公告)号：KR101751501B1
公开(公告)日：2017-07-11
申请号：KR1020150164050
申请日：2015-11-23
Applicant: 한국과학기술원
IPC: C07K16/18 , C07K16/28 , C07K14/195 , A61K38/16 , A61K47/48
CPC classification number: Y02A50/469 , Y02A50/471
Abstract: 본발명은리피바디(repebody)를이용한단백질독소복합체및 그제조방법에관한것으로, 상세하게는알파나선형캡핑모티프(capping motif)를가지는 LRR(leucine-rich repeat) 패밀리단백질의 N-말단에 VLR(variable lymphocyte receptor)에서기인한 LRR 구조가가변부위로연결되어기질특이성을나타내는리피바디에단백질독소와같은활성을갖는물질이접합된리피바디-단백질독소복합체 (Repebody-Protein toxin Conjugate)와이를제조하는방법및 그용도에관한것이다. 본발명에따른리피바디-단백질독소복합체는리피바디가제공하는높은기질특이성과우수한조직및 종양침투력과, 생물학적활성을지닌단백질독소가결합한것으로써, 리피바디단독으로사용하는경우에비해월등한효능을지니고, 단백질독소단독으로사용하는경우에비해특이적선택성을지니는장점을가지고있으며, 이를활용하여암세포에과발현되는단백질에특이적으로결합하는리피바디에, 세포를사멸시키는단백질독소를결합시켜암세포만을선택적으로사멸시킴으로써종양치료에유용하다.
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公开(公告)号：KR101308913B1
公开(公告)日：2013-09-23
申请号：KR1020110031737
申请日：2011-04-06
Applicant: 한국과학기술원
Abstract: 본 발명은 폴리도파민의 흡착력을 이용한 폐수 정제 방법 또는 오염물질 흡착 방법에 대한 것으로, 보다 상세하게는 폴리도파민을 코팅한 실리카비드를 제조, 이를 이용하여 폐수 등의 오염물질을 함유한 대상에 포함된 중금속, 유기 독성 물질 및 방사능 동위 원소와 같은 오염 물질을 제거하고, 상기 사용된 실리카비드에 산 또는 과산화수소를 처리하여 재사용이 가능하도록 하는 폐수 정제 또는 오염물질 흡착 방법, 이에 이용되는 폴리도파민 실리카비드의 제조 방법, 상기 제조 방법에 의해 제조된 폴리도파민 실리카비드 및 이 실리카비드의 용도에 관한 것이다. 본 발명은 폐수 정제를 위한 전처리 과정을 거치지 않아도 되고, 필터의 제조 과정이 비교적 간단하며, 수용액 상에서 유, 무기물의 제조가 간단하고, 필터의 재생 사용이 가능하므로 자원 및 비용 절감 효과와 함께 2차 오염의 문제를 해결할 수 있다.
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公开(公告)号：KR1020170059729A
公开(公告)日：2017-05-31
申请号：KR1020150164050
申请日：2015-11-23
Applicant: 한국과학기술원
IPC: C07K16/18 , C07K16/28 , C07K14/195 , A61K38/16 , A61K47/48
CPC classification number: Y02A50/469 , Y02A50/471
Abstract: 본발명은리피바디(repebody)를이용한단백질독소복합체및 그제조방법에관한것으로, 상세하게는알파나선형캡핑모티프(capping motif)를가지는 LRR(leucine-rich repeat) 패밀리단백질의 N-말단에 VLR(variable lymphocyte receptor)에서기인한 LRR 구조가가변부위로연결되어기질특이성을나타내는리피바디에단백질독소와같은활성을갖는물질이접합된리피바디-단백질독소복합체 (Repebody-Protein toxin Conjugate)와이를제조하는방법및 그용도에관한것이다. 본발명에따른리피바디-단백질독소복합체는리피바디가제공하는높은기질특이성과우수한조직및 종양침투력과, 생물학적활성을지닌단백질독소가결합한것으로써, 리피바디단독으로사용하는경우에비해월등한효능을지니고, 단백질독소단독으로사용하는경우에비해특이적선택성을지니는장점을가지고있으며, 이를활용하여암세포에과발현되는단백질에특이적으로결합하는리피바디에, 세포를사멸시키는단백질독소를결합시켜암세포만을선택적으로사멸시킴으로써종양치료에유용하다.
Abstract translation: 本发明涉及所述蛋白质毒素复合物的N-末端和使用中继器主体（repebody），特别是α-螺旋基序封端（封端基序）LRR（富亮氨酸重复），其具有VLR家族蛋白（制造方法 可变从淋巴细胞受体LRR结构结果）是具有活性的材料如蛋白质毒素给中继体示出与可变区域相关联的底物特异性接合中继体 - 生产蛋白质的毒素复合物（Repebody蛋白毒素缀合物）Y 其方法和用途。 根据本发明通过高底物特异性和优越的组织和肿瘤渗透，其主体设置有，具有生物活性的蛋白质毒素的组合，等等相比，使用转发器体单独月一个的情况下，蛋白质毒素复合中继器中继器体 具有这样的效果，通过组合具有与使用单个蛋白毒素的情况相比，具有特定选择性的优势，并且借此死亡在中继体特异性结合的蛋白，其在癌细胞中，在细胞中过表达的优势的蛋白质毒素 它仅通过选择性杀死癌细胞对肿瘤治疗有用。
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