公开(公告)号：KR101554385B1

公开(公告)日：2015-09-21

申请号：KR1020130030725

申请日：2013-03-22

Applicant: 한국과학기술원

Inventor： 박용근 ,  조상연 ,  장재덕 ,  이효철 ,  허원도

IPC: G01N33/52 ,  G01N33/53 ,  G01N21/64

Abstract: 본발명의실시예를형광물질영상화를위해형광공명에너지전이현상을이용하는방법과그 시스템에관한것이다. FRET 현상을이용한형광물질영상화방법에있어서, 적절한 FRET(Fluorescence resonance energy transfer) 페어를선정하고적합한현미경환경을설정하는단계; 관찰하고자하는시편에 FRET 페어중 도너(Donor)를주입하는단계; 도너주위에 FRET 페어중 억셉터(Acceptor)가존재하도록주입하거나발현시키는단계; FRET 페어를주입한시편의발광신호를촬영하는단계; 및발광신호를분석하여형광영상을생성하는단계를포함하는형광물질영상화방법이제공될수 있다.

公开(公告)号：KR1020140116305A

公开(公告)日：2014-10-02

申请号：KR1020130030725

申请日：2013-03-22

Applicant: 한국과학기술원

Inventor： 박용근 ,  조상연 ,  장재덕 ,  이효철 ,  허원도

IPC: G01N33/52 ,  G01N33/53 ,  G01N21/64

Abstract: One embodiment of the present invention relates to a method and a system for super resolution imaging based on fluorescence resonance energy transfer. The method for super resolution imaging based on fluorescence resonance energy transfer includes a step of selecting an appropriate FRET (Fluorescence resonance energy transfer) pair and setting a suitable microscope environment; a step of injecting a donor among a pair of FRET donor in a sample to be observed; a step of injecting or expressing an acceptor among FRET pairs around the donor; a step of recording the luminescence signal of the sample injected with the FRET pair; and a step of generating a fluorescent image by analyzing the luminescence signal.

Abstract translation: 本发明的一个实施例涉及一种基于荧光共振能量转移的超分辨率成像方法和系统。 基于荧光共振能量转移的超分辨率成像方法包括选择合适的FRET（荧光共振能量转移）对并设置合适的显微镜环境的步骤; 在待观察的样品中的一对FRET供体中注入供体的步骤; 在供体周围的FRET对中注射或表达受体的步骤; 记录注射了FRET对的样品的发光信号的步骤; 以及通过分析发光信号产生荧光图像的步骤。