公开(公告)号：KR100772078B1

公开(公告)日：2007-10-31

申请号：KR1020060109667

申请日：2006-11-07

Applicant: 한국기초과학지원연구원 ,  재단법인서울대학교산학협력재단

Inventor： 노정래 ,  김영환 ,  김은희 ,  이철현

IPC: C07H1/06

CPC classification number: C07H15/10 ,  C07H1/08

Abstract: Novel monogalactosyldiacylglycerol compounds isolated from Sargassum Thunbergii are provided which shows anti-fungal activity on Candida albican and contains both alpha-linoleic acid and eicosapentaenoic acid(EPA). A [(2S)-1-O-(5Z,8Z,11Z,14Z,17Z -eicosapentaenoyl)-2-O-(9Z,12Z,15Z-octadecatrienoyl)-3-O-beta-Dgalactopyranosyl-sn-glycerol] is represented by the formula(1). A (2S)-1-O-(9Z,12Z,15Z-octadecatrienoyl)-2-O-(6Z,9Z,12Z,15Z-octadecatetraenoyl)-3-O-beta-D-galactopyranosyl-sn-glycerol is represented by the formula(2). A method for preparing the monogalactosyldiacylglycerol compound comprises the steps of: (a) after mixing cut Sargassum Thunbergii with methanol in a weight ratio of 1:1-1:3 and leaving it for 48-72 hours, filtering it; (b) concentrating the methanol filtrate under reduced pressure and adding a mixture solution of butanol and water in a weight ratio of 1:1 thereto; (c) concentrating solvent of the butanol layer under reduced pressure and adding a mixture solution consisting of methanol and water in a weight ratio of 15:85 and normal hexane thereto; and (d) concentrating solvent of the methanol layer under reduced pressure and isolating the desired product through a reverse silica flash chromatography.

Abstract translation: 提供了从马齿苋（Thargarum Thunbergii）分离的新型单糖半乳糖二甘油化合物，其显示白念珠菌（Candida albican）的抗真菌活性，并含有α-亚油酸和二十碳五烯酸（EPA）。 A [（2S）-1-O-（5Z，8Z，11Z，14Z，17Z-二十碳烯酰基）-2-O-（9Z，12Z，15Z-十八碳三烯酰基）-3-O-β-D-吡喃半乳糖基-sn-甘油] 由式（1）表示。 代表了（2S）-1-O-（9Z，12Z，15Z-十八碳三烯酰基）-2-O-（6Z，9Z，12Z，15Z-十八碳四烯酰基）-3-O-β-D-吡喃半乳糖基-sn-甘油 通过式（2）。 制备单半乳糖基二酰基甘油化合物的方法包括以下步骤：（a）将切碎的马鞭草皂甙与甲醇以1：1-1：3的重量比混合48-72小时后，过滤; （b）减压浓缩甲醇滤液，加入重量比为1:1的丁醇和水的混合溶液; （c）在减压下浓缩丁醇层的溶剂，并加入由甲醇和水组成的重量比为15:85的混合溶液和正己烷; 和（d）减压浓缩甲醇层的溶剂，并通过反向硅胶快速色谱法分离所需产物。