公开(公告)号：KR1020150096866A

公开(公告)日：2015-08-26

申请号：KR1020140017680

申请日：2014-02-17

Applicant: 한국기초과학지원연구원 ,  조선대학교산학협력단

Inventor： 현재경 ,  송치홍 ,  정현석 ,  김시욱

IPC: C12M1/24 ,  C12M1/10

CPC classification number: C12M33/10

Abstract: 본 발명은 살아있는 세포를 손상 없이 원심분리를 통해 응집하기 위한 농축 시험관에 관한 것으로, 본 발명에 따르면, 한 번에 얻을 수 있는 용량이 작고 고가이며 모인 세포를 빼내기 어려운 종래의 2단 원심관을 이용한 방법 및 단가가 높고 내성이 약한 세포는 부서지기 쉬우며, 펠릿(pellet) 상태로 장시간 놓아두면 세포가 크게 손상을 입게 되는 종래의 원뿔형 튜브(conocal tube)를 이용한 방법의 문제점들을 해결하여, 플라스틱 재질로 이루어져 제작 및 취급이 간편하고 단가가 저렴한 동시에, 대량의 배양액에 존재하는 세포를 자동 원심분리기를 이용하여 손상 없이 농축시킬 수 있으며, 원심이 끝난 후 장시간 놓아두어도 세포의 손상이 일어나지 않도록 구성되는 세포 응집용 농축 시험관이 제공된다.

Abstract translation: 本发明涉及一种用于通过使用离心装置聚集活细胞而不损坏的浓缩管。 根据本发明，解决了使用现有的昂贵的两级离心管的方法的问题，其中一次只能获得少量的内容物，并且困难地取出聚集的电池，并且 使用现有的昂贵的锥形管的方法，其中具有低电阻的电池容易断裂，并且如果长时间留在颗粒相中，则细胞被显着损坏。 浓缩管：可以容易地制造和处理，并且由于浓缩管由塑料材料制成而成本低廉; 可以通过使用自动离心装置将存在大量培养液的细胞浓缩而不损伤; 并且即使细胞离心后长时间放置，也能防止细胞的损伤。

公开(公告)号：KR101594539B1

公开(公告)日：2016-02-16

申请号：KR1020140017680

申请日：2014-02-17

Applicant: 한국기초과학지원연구원 ,  조선대학교산학협력단

Inventor： 현재경 ,  송치홍 ,  정현석 ,  김시욱

IPC: C12M1/24 ,  C12M1/10

Abstract: 본발명은살아있는세포를손상없이원심분리를통해응집하기위한농축시험관에관한것으로, 본발명에따르면, 한번에얻을수 있는용량이작고고가이며모인세포를빼내기어려운종래의 2단원심관을이용한방법및 단가가높고내성이약한세포는부서지기쉬우며, 펠릿(pellet) 상태로장시간놓아두면세포가크게손상을입게되는종래의원뿔형튜브(conocal tube)를이용한방법의문제점들을해결하여, 플라스틱재질로이루어져제작및 취급이간편하고단가가저렴한동시에, 대량의배양액에존재하는세포를자동원심분리기를이용하여손상없이농축시킬수 있으며, 원심이끝난후 장시간놓아두어도세포의손상이일어나지않도록구성되는세포응집용농축시험관이제공된다.

公开(公告)号：KR101407234B1

公开(公告)日：2014-06-13

申请号：KR1020130151721

申请日：2013-12-06

Applicant: 한국기초과학지원연구원

Inventor： 정현석 ,  송치홍 ,  현재경 ,  전상미 ,  유동주 ,  박종필

IPC: H01J37/20 ,  H01J37/26

Abstract: The present invention relates to a liquid specimen holder of an electron microscope and an electron microscope using the same. According to the present invention, to solve problems of an existing electron microscope, for examples, the inconvenience of solidification and crystallization of a corresponding sample in a vacuum to monitor the liquid sample, the difficulty of monitoring the real image of the liquid sample in a liquid state due to the deformation of the sample, and the preparation of each sample according to a new condition whenever an experimental condition is changed, provided are the liquid sample holder of the electron microscope which protects a sample from vacuum and monitors the sample in a liquid state in real time and replaces a different experiment solution and injects an experiment solution such as culture fluid when the sample is mounted, thereby continuously monitoring the sample, and the electron microscope using the same.

Abstract translation: 本发明涉及一种电子显微镜的液体样本保持器和使用其的电子显微镜。 根据本发明，为了解决现有的电子显微镜的问题，例如，在真空中凝固和结晶相关样品以监测液体样品的不便之处，难以监测液体样品的真实图像 液体状态由于样品的变形，每当实验条件改变时，根据新的条件制备每个样品，提供了保护样品免受真空的电子显微镜的液体样品架，并在 液体状态，替代不同的实验溶液，并在样品安装时注入培养液等实验溶液，从而连续监测样品，使用电子显微镜。

公开(公告)号：KR101396420B1

公开(公告)日：2014-05-20

申请号：KR1020130151742

申请日：2013-12-06

Applicant: 한국기초과학지원연구원

Inventor： 정현석 ,  전상미 ,  현재경 ,  유동주 ,  송치홍 ,  박종필

IPC: G01N1/42 ,  G01N23/225

Abstract: The present invention relates to an apparatus for preparing a specimen containing a cryo stage for installing a grid cooled by liquefied nitrogen on a fluorescence microscope. The present invention provides an apparatus for preparing a correlative cryogenic specimen of an electron microscope of a novel structure which is formed in order to consecutively perform a pre-process of cooling the grid by inserting the grid into liquefied nitrogen and a series of processes of installing the grid on the fluorescence microscope by transferring the pre-processed grid and observing the grid within a single apparatus of preparing a specimen by solving the problems of an existing electron microscope in which the damage or breakage of the grid in a pre-processing of cooling the grid and a process of transferring the pre-processed grid and observing the grid is frequently generated, and a transporting operation of the grid is difficult and inconvenient since a temperature is constantly maintained during transferring the pre-processed grid.

Abstract translation: 本发明涉及一种用于制备含有用于在荧光显微镜上安装由液氮冷却的格栅的低温台的试样的装置。 本发明提供了一种新型结构的电子显微镜的相关低温试样的制备装置，其特征在于，为了通过将格栅插入液氮中连续地进行冷却网格的预处理，并且一系列安装过程 在荧光显微镜上通过转移预处理网格并在单个制备样品的装置内观察网格，通过解决现有电子显微镜的问题，其中在冷却的预处理中电网的损坏或破裂 频繁地产生电网和传送预处理电网并观察电网的过程，并且由于在传送预处理电网期间恒定地维持温度，电网的传输操作困难和不方便。

公开(公告)号：KR101388509B1

公开(公告)日：2014-04-28

申请号：KR1020130151602

申请日：2013-12-06

Applicant: 한국기초과학지원연구원

Inventor： 정현석 ,  현재경 ,  최의준 ,  전상미 ,  유동주 ,  송치홍

IPC: G01N1/42 ,  G01N33/48

Abstract: The present invention relates to a cryo plunger for cooling the sample grid to the temperature of liquid nitrogen which is to observe a biological sample using a transmission electron microscope (TEC) by a cryo analysis method, and according to the present invention, the present invention solves problems of conventional cryo sample plunger devices which are formed by a hydraulic device, the configuration is complicated, and manufacturing costs are high, and a spring type of a cryo sample plunger device which uses a spring structure thereby simplifying the configuration and correspondingly reducing manufacturing costs is provided.

Abstract translation: 本发明涉及一种用于将样品网格冷却至液氮温度的冷冻柱塞，该冷却柱塞使用透射电子显微镜（TEC）通过低温分析方法观察生物样品，根据本发明，本发明 解决了由液压装置形成的常规低温样品柱塞装置的问题，构造复杂，制造成本高，并且使用弹簧结构的冷冻样品柱塞装置的弹簧式，从而简化了构造并相应地减少制造 提供费用。