公开(公告)号：KR1020080100645A

公开(公告)日：2008-11-19

申请号：KR1020070046606

申请日：2007-05-14

Applicant: 한국식품연구원

Inventor： 최성욱 ,  전향숙 ,  장현주 ,  김현정 ,  김현정 ,  이나리 ,  김재호 ,  이재혁

IPC: G01N33/53 ,  G01N33/50 ,  G01N33/48

Abstract: A surface plasmon resonance sensor chip using molecular imprinting technique is provided to detect easily fungal toxin zearalenone or a derivative of the fungal toxin zearalenone using an electrochemical method. A surface plasmon resonance sensor chip using molecular imprinting technique for detecting easily fungal toxin zearalenone or a derivative of the fungal toxin zearalenone contains a substrate(10) in which a metal layer is formed, a metal layer(20) formed on a polymer layer including a binding site(21) combined with a zearalenone or a derivative of the zearalenone. The polymer layer is manufactured by steps of: mixing a monomer polymerized if electricity is applied and a template for forming a binding site in which the zearalenone or the derivative of the zearalenone is combined; forming a polymer layer by applying the electricity after injecting the monomer in which the template is mixed on the substrate; and forming the binding site combined with a zearalenone or a derivative of the zearalenone by removing the template from the polymer layer.

Abstract translation: 提供使用分子印迹技术的表面等离子体共振传感器芯片，以使用电化学方法检测玉米赤霉烯真菌毒素或玉米赤霉菌真菌毒素的衍生物。 使用分子印迹技术的表面等离子体共振传感器芯片容易地检测玉米赤霉烯真菌毒素或玉米赤霉菌素的衍生物的真菌毒素含有其中形成金属层的基底（10），在聚合物层上形成的金属层（20），包括 与玉米赤霉烯酮或玉米赤霉烯酮衍生物结合的结合位点（21）。 聚合物层通过以下步骤制造：混合施加电力时聚合的单体和用于形成玉米赤霉烯酮或玉米赤霉烯酮衍生物的结合位点的模板; 通过在将基板上混合有模板的单体注入后施加电力来形成聚合物层; 并通过从聚合物层去除模板，形成与玉米赤霉烯酮或玉米赤霉烯酮衍生物结合的结合位点。