公开(公告)号：KR100305747B1

公开(公告)日：2001-09-24

申请号：KR1019990011774

申请日：1999-04-03

Applicant: 한국식품연구원

Inventor： 윤칠석 ,  김인환 ,  정근기

IPC: A23D9/00

Abstract: PURPOSE: A process of preparing pure conjugated linoleic acid(CLC) by reacting vegetable oil rich in linoleic acid or linoleic acid in fatty acid state as a substrate under atmospheric pressure or under pressure is provided. By feeding of the CLC composition to livestock in a fatty acid state, animal products having a high content of functional CLC is produced. CONSTITUTION: Fat having a linoleic acid content of 40% or more is dissolved in ethylene glycol and reacted with alkali such as caustic soda or soda ash at 150 to 190deg.C for 30 min to 2.5 hr at 5 to 250psi in 1,000 to 500ml/min nitrogen gas and 30 to 15ml/min hydrogen gas. The reactant is separated by adjusting the pH of the solution to 4.5 to 5.0 and then esterificated. A solution of sulfuric anhydride and ethanol is added to conjugated linoleic acid and then reacted. Oxides contained in the solution are extracted by use of supercritical carbon dioxide.

公开(公告)号：KR1019990073077A

公开(公告)日：1999-10-05

申请号：KR1019990011774

申请日：1999-04-03

Applicant: 한국식품연구원

Inventor： 윤칠석 ,  김인환 ,  정근기

IPC: A23D9/00

Abstract: 본발명은리놀레인산(Linoleic acid) 함량이풍부한식용유지를원료로하여이성화(isomerization) 반응시켜공역화리놀레인산(CLA: Conjugated Linoleic Acid)을제조하는방법에관한것이다. 종래의공역화리놀레인산은지방산화물함량이 15∼20%로높으며 cis-9와 trans-11의 CLA 및 trans-10와 cis-12의 CLA 간의상호함량비율을조절할수 없었다. 본발명은기본적인반응조성물의함량대비알칼리농도, pH조절에의한 CLA의분리, 최적압력반응조건, 및산화물의제거방법등으로구성하여반응원료인리놀레인산을 98%이상 CLA (cis-9, trans-11 및 trans-10, cis-12)로전환시키며, 지방산화물이제거된순수한 CLA제품및 cis-9, trans-11과 trans-10, cis-12 CLA의상호비율을조절하는방법을제공하고자한다. 따라서본 발명은양질의 CLA를신속하고효율적으로제조하여단위(單胃)가축의배합사료에 0.3% 이상첨가하여급여하면어린가축의성장촉진효과를얻을수 있고, 산란계및 돼지에급여시 천연 CLA 함량이풍부한축산물의생산, 계란의난각질강화및 계란의난황및 돼지의등지방에서글리세라이드형태의천연 CLA가함유된난황유및 돈지를얻을수 있다. 또한단위가축으로서토종개, 육용오리, 메추리용 CLA사료조성물에적용할때도지방및 콜레스테롤함량이아주적고 CLA함량이높은고기및 알을얻을수 있다.

Abstract translation: 目的：提供在大气压或压力下使富含亚油酸或亚油酸的植物油以脂肪酸状态作为底物的纯共轭亚油酸（CLC）的制备方法。 通过将CLC组合物喂入脂肪酸状态的家畜，生产具有高功能CLC含量的动物产品。 组成：将亚油酸含量为40％以上的脂肪溶解在乙二醇中，在150〜190℃下与碱如苛性钠或苏打灰反应30分钟〜2.5小时，以5〜250psi，1000〜500ml / min氮气和30〜15ml / min氢气。 通过将溶液的pH调节至4.5至5.0然后酯化来分离反应物。 将硫酸酐和乙醇的溶液加入到共轭亚油酸中，然后反应。 通过使用超临界二氧化碳提取溶液中所含的氧化物。