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公开(公告)号：KR1020030018168A
公开(公告)日：2003-03-06
申请号：KR1020010051749
申请日：2001-08-27
Applicant: 한국식품연구원
IPC: A61K39/395
Abstract: PURPOSE: A method for producing eggs containing an anti-insulin antibody is provided, thereby producing eggs containing specific immunoglobulin yolk(IgY) for a long time. CONSTITUTION: The method for producing eggs containing an anti-insulin antibody comprises the steps of: pre-treating human insulin and conjugating the pre-treated human insulin to a carrier to prepare the insulin-protein conjugate; periodically injecting the conjugate together with an immune adjuvant and an emulsifying agent to laying hens, wherein the pre-treatment of human insulin is carried out by dialysis of human insulin with a 3,500Da membrane at 4deg. C for 24 hours; the carrier is ovalbumin or bovine serum albumin; EDAC(1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) is coupled with the insulin-protein conjugate; and FCA(Freund's complete adjuvant) is used as an emulsifying agent in initial immunization and FIA(Freund's incomplete adjuvant) is used as another emulsifying agent in later immunization.
Abstract translation: 目的：提供含有抗胰岛素抗体的卵的生产方法，由此产生长时间含有特异性免疫球蛋白（IgY）的卵。 构成：含有抗胰岛素抗体的蛋的生产方法包括以下步骤：预处理人胰岛素并将预处理的人胰岛素与载体缀合以制备胰岛素 - 蛋白质缀合物; 将结合物与免疫佐剂和乳化剂定期注射到产蛋鸡，其中人胰岛素的预处理是通过用4,500g的3,500Da膜透析人胰岛素进行的。 C 24小时; 载体为卵白蛋白或牛血清白蛋白; EDAC（1-乙基-3-（3-二甲基氨基丙基）碳二亚胺）与胰岛素 - 蛋白质结合物偶联; 和FCA（弗氏完全佐剂）在初次免疫中用作乳化剂，并且在后续免疫中使用FIA（弗氏不完全佐剂）作为另外的乳化剂。
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公开(公告)号：KR100795556B1
公开(公告)日：2008-01-21
申请号：KR1020010051749
申请日：2001-08-27
Applicant: 한국식품연구원
IPC: A61K39/395
Abstract: 본 발명은 항인슐린 항체를 함유하는 계란의 생산방법에 관한 것으로서, 계란으로부터 항인슐린 항체를 생산하기 위하여 인간 인슐린을 전처리하여 담체와 함께 결합체를 형성시킨 후, 면역보강제와 유화물을 만들어 산란계에 주기적으로 면역주사함으로써 일정기간 지난 후 항인슐린 항체가 다량 포함된 계란을 생산하는 것을 특징으로 하는 항인슐린 항체 계란의 생산방법을 제공한다.
항인슐린 항체, 계란
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