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公开(公告)号：KR101413371B1
公开(公告)日：2014-07-01
申请号：KR1020120109707
申请日：2012-10-02
Applicant: 한국해양과학기술원
Abstract: 본 발명은 각각 파장이 다른 LED(light-emitting diode)를 미세조류배양에 적용하여, 해수(해양심층수 및 해양심층수 처리수)에서 배양된 두나리엘라(Dunaliella)의 색소 및 성분 변화에 대한 배양기법에 관한 것으로서, 더욱 상세하게는 해수(해양심층수 및 해양심층수 처리수)를 배양액으로 하는 제조 단계, 해수(해양심층수 및 해양심층수 처리수)에 두나리엘라를 배양시키는 단계, 각기 다른 파장에서 두나리엘라를 배양하는 단계, 두나리엘라에 스트레스를 가해 체내 클로로필, 베타카로틴 등 색소 성분의 농도를 높이는 단계를 포함하는 미세조류(두나리엘라)의 배양기법에 관한 것이다.
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公开(公告)号：KR1020140044419A
公开(公告)日：2014-04-15
申请号：KR1020120109707
申请日：2012-10-02
Applicant: 한국해양과학기술원
Abstract: The present invention relates to a culture technique which is in regard to changes in the pigment and ingredient of Dunaliella cultured in sea water(deep sea water and treated deep sea water) by applying light-emitting diodes (LEDs) different in wavelength to the culture of microalgae. More specifically, the present invention relates to a culture technique for microalgae(Dunaliella) comprising the steps of: preparing sea water(deep sea water and treated deep sea water) as a culture medium; culturing Dunaliella in the sea water(deep sea water and treated deep sea water); culturing the Dunaliella in different wavelengths; and adding stress to the Dunaliella to increase the concentration of a pigment ingredient such as chlorophyll, beta-carotene, etc. [Reference numerals] (AA) Sea water(surface layer water, deep sea water and treated deep sea water); (BB) Compose a culture medium; (CC) Include a chelating agent in the culture medium; (DD) Filter the culture medium; (EE) Disinfect/sterilize the culture medium; (FF) UV treatment or thermal treatment; (GG) Make a culture environment; (HH) Adjust salinity, temperature, etc.; (II) Culture microalgae; (JJ) Adjust wavelengths by using LEDs; (KK) Blue wavelength; (LL) Produce microalgae with increased chlorophyll contents; (MM) Red wavelength; (NN) Produce microalge with increased carotinoid contents; (OO) Produce microalge with increased beta-carotene contents
Abstract translation: 本发明涉及通过将不同波长的发光二极管（LED）施加到培养物中，涉及在海水中培养的杜氏盐藻（深海水和经处理的深海水）的颜料和成分的变化的培养技术。 的微藻。 更具体地说，本发明涉及微藻（杜氏盐藻）的培养技术，包括以下步骤：制备海水（深海水和经处理的深海水）作为培养基; 在海水中培养杜氏盐藻（深海水处理深海水）; 培养不同波长的杜氏盐藻; 并加入杜氏盐藻中以增加颜料成分如叶绿素，β-胡萝卜素等的浓度。（附图标记）（AA）海水（表层水，深海水和经处理的深海水）; （BB）组成培养基; （CC）在培养基中包含螯合剂; （DD）过滤培养基; （EE）消毒/灭菌培养基; （FF）紫外线处理或热处理; （GG）创造文化氛围; （HH）调节盐度，温度等; （二）培养微藻; （JJ）使用LED调整波长; （KK）蓝色波长; （LL）生产具有增加的叶绿素含量的微藻; （MM）红色波长; （NN）产生具有增加的类胡萝卜素含量的微量化物; （OO）产生具有增加的β-胡萝卜素含量的微量化
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