Abstract:
본 발명은 기능성 전분 및 지질을 고농도로 축적하는 신규 미세조류 클로렐라(Chlorella sp.)에 관한 것으로, 본 발명에 따른 클로렐라 ArM29B 세포주는 배양 중에 고농도로 전분 및 지질을 축적하는 세포주로 확인되었으며, 다양한 온도조건에서 배양이 가능하며, 세포 내 중성기름 방울을 특이적으로 염색할 수 있는 나일 레드(nile red) 분석법으로 고농도의 지질을 축적하는 것을 확인함으로써, 바이오디젤 및 기능성 지질생산의 소재로 사용될 수 있다. 또한 본 세포주 클로렐라 ArM29B 세포주는 배양 중에 지질을 고농도로 축적하므로 바이오디젤용 미세조류로 적당하고, 결빙온도 이상에서는 모두 잘 자라므로 배양시 온도 조건을 특별히 맞출 필요가 없고, 봄 여름 가을 겨울 모두 잘 자라므로 연중 배양 및 생산이 가능하다.
Abstract:
The present invention relates to new microalgae chlorella (Chlorella sp.) for accumulating high concentration functional starch and lipid. According to the present invention, a chlorella ArM29B cell line is confirmed as a cell line for accumulating high concentration starch and lipid in culturing, and cultured in various temperature conditions. Moreover, a nile red analysis method capable of specifically dyeing a neutral oil drop inside a cell confirms that high concentration lipid is accumulated. Therefore, the cell line can be used as a material of producing functional lipid and biodiesel. The chlorella ArM29B cell line accumulates the high concentration lipid in culturing, so the cell line is suitable for microalgae for biodiesel. All chlorella ArM29B cell lines are growing well above a freezing temperature, so there is no necessity for a specific temperature condition in culturing. All chlorella ArM29B cell lines are growing well in four seasons, so the cell line is produced and cultured throughout the year.
Abstract:
PURPOSE: A novel microalgae Chlamydomonas ArM29 is provided to accumulate lipid of high concentration. CONSTITUTION: A Chlamydomonas sp. strain is derived from arctic ocean and produces lipid of high concentration. The strain is Chlamydomonas sp. ArM29(KCTC 11695BP). The strain accumulates lipid of high concentration in a lipid body form. The temperature of culturing the strain is 0°C-30°C. A microorganism formulation for producing lipid contains the strain or culture liquid thereof as an active ingredient.