公开(公告)号：KR100304789B1

公开(公告)日：2001-09-24

申请号：KR1019990029933

申请日：1999-07-23

Applicant: 한국해양연구원

Inventor： 이홍금 ,  이정현 ,  고성환 ,  박신혜 ,  이득수 ,  이현상

IPC: C12N1/20

Abstract: 본발명은세포외로폴리사카라이드를분비하는) 96CJ10356 균주(KCTC 0564BP) 및이로부터엑소폴리사카라이드를생산하는방법에관한것으로, 본발명에의해생산된엑소폴리사카라이드(exopolysaccharide)는글루코스와갈락토스가 1:6.8의비율로존재하고, 분자량이 2.2×10인폴리사카라이드로서수용액에녹고, 응집활성을가지며, 유화안정성이우수하므로, 식품산업등과같은다양한산업분야에서이용될수 있다.

公开(公告)号：KR1020010010830A

公开(公告)日：2001-02-15

申请号：KR1019990029933

申请日：1999-07-23

Applicant: 한국해양연구원

Inventor： 이홍금 ,  이정현 ,  고성환 ,  박신혜 ,  이득수 ,  이현상

IPC: C12N1/20

Abstract: PURPOSE: A microorganism Aeromonas sp. 96CJ10356 and a method for producing exopolysaccharide from the same are provided. Thereby, the exopolysaccharide which may be dissolved not in organic solvents but in water and which is useful as a coagulant and an emulsifying stabilizer can be produced. CONSTITUTION: The Aeromonas sp. 96CJ10356 is isolated and identified by the following steps: suspending samples obtained from the coasts of Cheju and Mara islands; spreading the suspended solution on ZoBell solid medium; incubating them at room temperature to isolate microorganisms having viscosity; inoculating the microorganisms into YMG liquid medium; incubating them with agitation of 120 rpm at 25deg.C for 7 days; centrifuging the fermented solution; adding ethanol into the supernatant to precipitate exopolysaccharides; collecting and drying the exopolysaccharides and measuring their weights; and selecting a microorganism, Aeromonas sp. 96CJ10356 producing the most amount of exopolysaccharide.

Abstract translation: 目的：微生物气单胞菌 96CJ10356及其制备多糖的方法。 因此，可以制造不溶于有机溶剂但在水中可用作凝结剂和乳化稳定剂的外多糖。 构成：气单胞菌属 96CJ10356通过以下步骤进行隔离和鉴定：悬挂从济州海域和马拉群岛获得的样品; 将悬浮液撒在ZoBell固体培养基上; 在室温下孵育以分离具有粘度的微生物; 将微生物接种到YMG液体培养基中; 在25℃下搅拌120rpm培养7天; 离心发酵溶液; 向上清液中加入乙醇沉淀外多糖; 收集和干燥外多糖并测量其重量; 并选择微生物气单胞菌属 产生大量外多糖的96CJ10356。