公开(公告)号：KR1020040071991A

公开(公告)日：2004-08-16

申请号：KR1020030007918

申请日：2003-02-07

Applicant: 한국해양연구원

Inventor： 이홍금 ,  이유경 ,  김효원 ,  조경희 ,  임정한

IPC: C12N15/31

CPC classification number: C07K14/36 ,  C12N15/70

Abstract: PURPOSE: A cold shock protein gene isolated from Streptomyces sp. AA8321 and uses thereof are provided, thereby increasing survival rate of useful microorganisms and improving low temperature tolerance of lactic acid bacteria or low temperature storage required useful microorganisms. CONSTITUTION: The cold shock protein gene csp isolated from Streptomyces sp. AA8321(KCTC 0518BP) has the nucleotide sequence set forth in SEQ ID NO:1. The cold shock protein isolated from Streptomyces sp. AA8321(KCTC 0518BP) has the amino acid sequence set forth in SEQ ID NO:2. The method for producing the cold shock protein comprises the steps of: constructing a recombinant expression vector containing the cold shock protein gene csp of SEQ ID NO:1; transforming E. coli with the recombinant expression vector containing the cold shock protein gene csp of SEQ ID NO:1 to produce E. coli LMG194(KCTC 10354BP); and culturing E. coli LMG194(KCTC 10354BP).

Abstract translation: 目的：从Streptomyces sp。分离的冷休克蛋白基因。 提供了AA8321及其用途，从而提高了有用微生物的存活率，提高了乳酸菌的低温耐受性或需要有用微生物的低温贮藏。 构成：从Streptomyces sp。分离的冷休克蛋白基因csp AA8321（KCTC 0518BP）具有SEQ ID NO：1所示的核苷酸序列。 从链霉菌分离的冷休克蛋白 AA8321（KCTC 0518BP）具有SEQ ID NO：2所示的氨基酸序列。 制备冷休克蛋白的方法包括以下步骤：构建含有SEQ ID NO：1的冷休克蛋白基因csp的重组表达载体; 用含有SEQ ID NO：1的冷休克蛋白基因csp的重组表达载体转化大肠杆菌以产生大肠杆菌LMG194（KCTC 10354BP）; 并培养大肠杆菌LMG194（KCTC 10354BP）。