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公开(公告)号：KR1020130015587A
公开(公告)日：2013-02-14
申请号：KR1020110077648
申请日：2011-08-04
Applicant: 한남대학교 산학협력단
Abstract: PURPOSE: A method for manufacturing a multifunctional biosensor material and a high efficiency biosensor are provided to obtain the biosensor with storage stability regardless of temperature and humidity. CONSTITUTION: A method for manufacturing a multifunctional biosensor material comprises: a step of dispersing biomaterial affinity vinyl monomers and vinyl ferrocene throughout a methanol solution; a step of irradiating the methanol solution by radiation and generating radical; and a step of grafting a biomaterial affinity functional group and an electron transport function to the surface of a carbon nanotube. The biomaterial affinity vinyl monomer is acrylic acid, methacrylic acid, glycidyl methacrylate, maleic anhydride, 2-hydroxy methacrylate, 4-vinylphenylboronic acid, sodium styrenesulfonate, imidazolate containing a vinyl group, vinyl pyrrolidone, or an amino acid containing the vinyl group.
Abstract translation: 目的：提供一种制造多功能生物传感器材料和高效生物传感器的方法，以获得具有储存稳定性的生物传感器，而不管温度和湿度如何。 构成：制造多功能生物传感器材料的方法包括：将生物材料亲和乙烯基单体和乙烯基二茂铁分散在整个甲醇溶液中的步骤; 通过辐射照射甲醇溶液并产生自由基的步骤; 以及将生物材料亲和官能团和电子传递功能接枝到碳纳米管的表面的步骤。 生物材料亲和性乙烯基单体是丙烯酸，甲基丙烯酸，甲基丙烯酸缩水甘油酯，马来酸酐，2-羟基甲基丙烯酸酯，4-乙烯基苯基硼酸，苯乙烯磺酸钠，含有乙烯基的咪唑酸酯，乙烯基吡咯烷酮或含有乙烯基的氨基酸。
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公开(公告)号：KR101274854B1
公开(公告)日：2013-06-13
申请号：KR1020100133021
申请日：2010-12-23
Applicant: 한남대학교 산학협력단
IPC: C12Q1/68 , G01N27/327
Abstract: 본 발명은 감염성 호흡기 질환 진단용 전기화학 DNA 센서 및 이의 제조방법에 관한 것이다. 본 발명의 전기화학 DNA 센서는 다양한 작용기를 갖는 라디칼 발생 분자를 제조한 후, 전기를 가하여 라디칼 반응을 통해 탄소전극 또는 금속전극 표면에 다양한 작용기를 도입하고, 이 작용기에 아비딘을 공유결합시킨 다음 인플루엔자 바이러스 염기서열을 갖는 비오티닐화된 프로브 DNA를 결합시켜 전극에 고정화시킴으로써, 감도가 높고 간섭효과가 매우 작다. 따라서, 본 발명의 전기화학 DNA 센서는 인플루엔자 바이러스를 간단히, 신속히, 편리하게 측정하여 현장에서 호흡기 질환 감염자의 일차 분류를 위한 스크리닝 검사와 정확한 처방을 위한 확진 판정용 센서로서 유용하게 사용될 수 있다.
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公开(公告)号：KR1020120071463A
公开(公告)日：2012-07-03
申请号：KR1020100133021
申请日：2010-12-23
Applicant: 한남대학교 산학협력단
IPC: C12Q1/68 , G01N27/327
CPC classification number: C12Q1/6888 , C12Q2565/619 , G01N27/3278 , G01N33/56983
Abstract: PURPOSE: An electrochemical DNA sensor for diagnosing infectious respiratory diseases is provided to enhance sensitivity and to conveniently and quickly measure influenza virus. CONSTITUTION: A method for manufacturing an electrochemical DNA sensor for diagnosing infectious respiratory diseases comprises: a step of reacting an amine compound of chemical formula 1 and NaNO_2 in distilled water under the presence of an acid catalyst to prepare diazonium salt compounds of chemical formula 2; a step of dissolving the compounds of chemical formula 2 in an acetonitrile solution containing 0.1 M NBu4BF4(tetrabutylammonium tetrafluoroborate) and dipping carbon electrodes or metal electrodes in the solution; a step of applying -1.0 to 0.5V of current at room temperature to introduce a functional group on the electrode surface; a step of dissolving EDC(N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride) and NHS(N-Hydroxysuccinimide) in a phosphate buffer solution and dipping the functional group-introduced electrodes into the buffer solution to prepare a EDC/NHS-functional group-introduced electrodes; a step of dissolving avidin in a phosphate buffer solution and dipping the EDC-NHS-functional group-introduced electrode into the buffer solution to prepare avidin-functional group-introduced electrode; and a step of dipping the avidin-functional group-introduced electrode in a biotinylated probe DNA solution.
Abstract translation: 目的：提供用于诊断感染性呼吸系统疾病的电化学DNA传感器，以提高敏感性，方便快捷地测量流感病毒。 构成：用于诊断感染性呼吸系统疾病的电化学DNA传感器的制造方法，其特征在于，在酸催化剂的存在下，使化学式1的胺化合物与NaNO_2在蒸馏水中反应，制备化学式2的重氮盐化合物的步骤; 将化学式2的化合物溶解在含有0.1M NBu4BF4（四丁基四氟硼酸盐）的乙腈溶液中并将碳电极或金属电极浸渍在溶液中的步骤; 在室温下施加-1.0〜0.5V的电流以在电极表面上引入官能团的步骤; 将EDC（N-（3-二甲基氨基丙基）-N'-乙基碳二亚胺盐酸盐）和NHS（N-羟基琥珀酰亚胺）溶解在磷酸盐缓冲溶液中并将引入官能团的电极浸入缓冲溶液中以制备EDC / NHS 功能组引入电极; 将抗生物素蛋白溶解在磷酸盐缓冲溶液中并将引入EDC-NHS官能团的电极浸入缓冲溶液中以制备引入亲和素官能团的电极的步骤; 以及将生物素标记的探针DNA溶液中浸渍了抗生物素蛋白官能团导入的电极的步骤。
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公开(公告)号：KR1020100022165A
公开(公告)日：2010-03-02
申请号：KR1020080080702
申请日：2008-08-19
Applicant: 한남대학교 산학협력단
Abstract: PURPOSE: An enzyme-free glucose sensor and a method for manufacturing the same are provided to improve the sensitivity of a glucose sensor by coating boronic acid on carbon nanotube reacting with the diol group of glucose. CONSTITUTION: A manufacturing method of an enzyme-free glucose sensor comprises the steps of: dispersing a monomer including refined carbon nanotube and borate group into hydrophilic solvent, creating radical by radiation, grafting the monomer including the borate group on the surface of the carbon nanotube, and mixing the functional carbon nanotube and a polymer binder to complete the glucose sensor.
Abstract translation: 目的：提供一种无酶葡萄糖传感器及其制造方法，以通过将硼酸包覆在与二醇葡萄糖基团反应的碳纳米管上来提高葡萄糖传感器的灵敏度。 构成：无酶葡萄糖传感器的制造方法包括以下步骤：将包含精制碳纳米管和硼酸酯基团的单体分散在亲水溶剂中，通过辐射产生自由基，在碳纳米管的表面上接枝包含硼酸酯基团的单体 并混合功能性碳纳米管和聚合物粘合剂以完成葡萄糖传感器。
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