公开(公告)号：KR101164401B1

公开(公告)日：2012-07-12

申请号：KR1020090082877

申请日：2009-09-03

Applicant: 한림대학교 산학협력단

Inventor： 송원근 ,  정석훈 ,  김재석 ,  김한성 ,  이규만

IPC: C12N1/02 ,  C12N1/20 ,  C12P21/00

Abstract: 본 발명은 기질확장형 β-락타메이즈(ESBL) 또는 플라스미드 매개 AmpC β-락타메이즈(PABL)를 신속하고 정확하게 분류하고 탐지하는 액체배지 미량희석법에 관한 것이다.
액체배지 미량희석법(Broth microdilution), 클라뷸란산(clavulanic acid), 보론산(boronic acid), 기질확장성 β-락타메이즈(extended-spectrum β-lactamase, ESBL), 플라스미드 매개 AmpC β-락타메이즈(PABL)

公开(公告)号：KR1020110024751A

公开(公告)日：2011-03-09

申请号：KR1020090082877

申请日：2009-09-03

Applicant: 한림대학교 산학협력단

Inventor： 송원근 ,  정석훈 ,  김재석 ,  김한성 ,  이규만

IPC: C12N1/02 ,  C12N1/20 ,  C12P21/00

Abstract: PURPOSE: A method for quickly and accurately detecting extended-spectrum beta-lactamase by broth microdilution is provided. CONSTITUTION: A strain which produces extended spectrum beta-lactamase by broth microdilution is detected using: a combination of cefotaxime-PABL inhibitor broth and cefotaxim-ESBL inhibitor-PABL inhibitor broth; a combination of ceftazidime-PABL inhibitor broth and ceftazidime-ESBL inhibitor-PABL inhibitor broth; a combination of cefepime-PABL inhibitor broth and cefepime-ESBL inhibitor-PABL inhibitor broth; a combination of ceftriaxone-PABL inhibitor broth and ceftriaxone-ESBL inhibitor-PABL inhibitor broth; or a combination of ceftriaxone-PABL inhibitor broth and ceftriaxone-ESBL inhibitor-PABL inhibitor broth. The PABL inbibitor is boronic acid or cloxacilin containign phenylboronic acid and 3-aminophenyl boronic acid.

Abstract translation: 目的：提供通过肉汤微量稀释快速准确地检测扩展型β-内酰胺酶的方法。 构成：通过肉汤微量稀释产生广谱β-内酰胺酶的菌株使用头孢噻肟-PABL抑制剂肉汤和头孢噻肟-ESBL抑制剂-PABL抑制剂肉汤的组合进行检测; 头孢他啶-PABL抑制剂肉汤和头孢他啶-ESBL抑制剂-PABL抑制剂肉汤的组合; 头孢匹啶-PABL抑制剂肉汤和头孢吡肟-ESBL抑制剂-PABL抑制剂肉汤的组合; 头孢曲松-PABL抑制剂肉汤和头孢曲松-ESBL抑制剂-PABL抑制剂肉汤的组合; 或头孢曲松-PABL抑制剂肉汤和头孢曲松-ESBL抑制剂-PABL抑制剂肉汤的组合。 PABL抑制剂是硼酸或氯沙星含有苯基硼酸和3-氨基苯基硼酸。

公开(公告)号：KR101389696B1

公开(公告)日：2014-05-07

申请号：KR1020120094634

申请日：2012-08-29

Applicant: 한림대학교 산학협력단

Inventor： 송원근 ,  정석훈

IPC: C12Q1/04 ,  C12N1/20

Abstract: 본발명은다양한 &bgr;-락타메이즈저해제를이용하여그람음성간균임상분리물에서클렙시엘라뉴모니애카바페네메이즈 (KPCs) 및메탈로-&bgr;-락타메이즈 (MBLs) 생산을탐지하기위한액체배지미량희석법에관한것이다. 카바페네메이즈탐지시험법은이미페넴 (imipenem) 또는메로페넴 (meropenem)의순차적인 2배희석액을포함하고, 아미노페닐보론산 (APB), 페닐보론산 (PB), 클록사실린 (CLX), 디피콜린산 (DPA) 또는 EDTA를포함하거나포함하지않는 Mueller-Hinton 액체배지로이루어졌으며, KPC가있는 31개의클렙시엘라뉴모니애균주, 21개의 MBL 생산균주및 포린이손실되고플라스미드 AmpC 생산또는염색체 AmpC 과다생산을하는 16개의엔테로박테리아세아균주에대해평가하였다. 이미페넴또는메로페넴단독처리한경우와비교하여 &bgr;-락타메이즈저해제존재하에이미페넴또는메로페넴의최소생육저해농도는 3배또는그 이상감소하였고, 이러한결과를양성결과로판단하였다. 페닐보론산을넣거나또는넣지않고이미페넴으로본 발명의액체배지미량희석법을이용하여얻어진결과는클렙시엘라뉴모니애탐지에서음성 CLX 결과라는부가적인기준을포함하면, KPC 효소에가장필적할만한민감도 (94%) 및특이도 (95%)를나타내었다. DPA와 EDTA는모두 MBL 생산그람음성간균탐지에탁월한민감도 (DPA vs. EDTA가있거나또는없이이미페넴처리는 90% vs. 95%; DPA vs. EDTA가있거나또는없이메로페넴처리는 95% vs. 86%)와특이도 (DPA vs. EDTA가있거나또는없이이미페넴처리는 98% vs. 100%; DPA vs. EDTA가있거나또는없이메로페넴처리는 98% vs. 98%)를보였다. 본발명은페닐보론산, 클록사실린및 EDTA를가하거나가하지않고실시한이미페넴을이용한액체배지미량희석시험법이 KPC 및 MBL 효소탐지에가장효과적인결과를제공함을보여주었다.

公开(公告)号：KR1020140030429A

公开(公告)日：2014-03-12

申请号：KR1020120094634

申请日：2012-08-29

Applicant: 한림대학교 산학협력단

Inventor： 송원근 ,  정석훈

IPC: C12Q1/04 ,  C12N1/20

Abstract: The present invention relates to a liquid culture medium microdilution method to detect the production of Klebsiella pneumoniae cabapenemases (KPCs) and metalo-beta-lactamases (MBLs) from clinically separated gram-negative bacillus using various beta-lactamase inhibitors. The cabapenemase detection method is made of a Mueller-Hinton liquid culture medium which includes a diluted solution obtained by preforming a serial doubling dilution on imipenem or meropenem, and which includes or does not include aminophenyl-boronic (APB) acid, phenyl-boronic (PB) acid, cloxacillin (CLX), dipicolinic acid (DPA), or EDTA. The present invention proves that the liquid culture medium microdilution method, which uses imipenem, includes or does not include phenyl-boronic acid, cloxacillin, and EDTA, provides the most effective result of KPC and MBL detection.

Abstract translation: 本发明涉及使用各种β-内酰胺酶抑制剂从临床分离的革兰氏阴性芽孢杆菌中检测肺炎克雷伯杆菌血清白蛋白酶（KPC）和金属β-内酰胺酶（MBL）的产生的液体培养基微量稀释法。 卡培缩酶检测方法由Mueller-Hinton液体培养基制成，该培养基包括通过对亚胺培南或美罗培南进行连续倍增稀释获得的稀释溶液，其包括或不包括氨基苯基硼酸（APB）酸，苯基硼酸（ PB）酸，氯沙西林（CLX），二吡啶甲酸（DPA）或EDTA。 本发明证明使用亚胺培南的液体培养基微量稀释法包括或不包括苯基硼酸，氯沙西林和EDTA，提供了KPC和MBL检测最有效的结果。