公开(公告)号：WO2015066534A1

公开(公告)日：2015-05-07

申请号：PCT/US2014/063544

申请日：2014-10-31

Applicant: 3SCAN INC.

Inventor： GOODMAN, Matthew ,  DANIEL, Cody

IPC: G01N1/06

CPC classification number: G02B21/362 ,  B26D5/007 ,  G01N1/06 ,  G01N1/286 ,  G01N21/55 ,  G01N2001/2873 ,  G01N2201/02 ,  G01N2201/06113 ,  G02B21/002 ,  G02B21/32

Abstract: Motion strategies in two and three dimensions for scanning microscope imaging are described. An object, sample, or specimen is mounted on a precision three- dimensional stage. The object is moved concurrently with respect to a first axis and a second axis orthogonal to the first against a cutting tool to cut the object. An image of the cut portion is generated as the object is moved. The cutting tool may act as an optical waveguide for illuminating the portion of the object cut. An optical element captures images of the cut and illuminated object. The object may further be concurrently moved with respect to a third axis orthogonal to both the first and second.

Abstract translation: 描述扫描显微镜成像的二维和三维运动策略。 物体，样品或样品安装在精密三维平台上。 该物体相对于第一轴线和与第一轴线正交的第二轴线相对于切割工具同时移动以切割物体。 当物体移动时，产生切割部分的图像。 切割工具可以用作用于照射被切割部分的光波导。 光学元件捕获切割和照射的物体的图像。 该物体可以进一步相对于与第一和第二两者正交的第三轴同时移动。

公开(公告)号：WO2019191479A1

公开(公告)日：2019-10-03

申请号：PCT/US2019/024658

申请日：2019-03-28

Applicant: 3SCAN INC.

Inventor： RHODES, Christopher ,  DANIEL, Cody ,  SPRINGER, Joey ,  PARKER, Kristy

IPC: G01N1/28 ,  G01N1/30

Abstract: An apparatus and a method for automated slicing, staining, and imaging of tissue section are provided. The apparatus comprises a tissue sample carrier that moves a tissue sample along a path, a continuous substrate that adheres to a face of the tissue sample, a knife that slices the tissue sample, an applicator that applies pressure to the continuous substrate and an imaging device.

公开(公告)号：EP3063523A1

公开(公告)日：2016-09-07

申请号：EP14857971.7

申请日：2014-10-31

Applicant: 3Scan Inc.

Inventor： GOODMAN, Matthew ,  DANIEL, Cody

IPC: G01N1/06

CPC classification number: G02B21/362 ,  B26D5/007 ,  G01N1/06 ,  G01N1/286 ,  G01N21/55 ,  G01N2001/2873 ,  G01N2201/02 ,  G01N2201/06113 ,  G02B21/002 ,  G02B21/32

Abstract: Motion strategies in two and three dimensions for scanning microscope imaging are described. An object, sample, or specimen is mounted on a precision three- dimensional stage. The object is moved concurrently with respect to a first axis and a second axis orthogonal to the first against a cutting tool to cut the object. An image of the cut portion is generated as the object is moved. The cutting tool may act as an optical waveguide for illuminating the portion of the object cut. An optical element captures images of the cut and illuminated object. The object may further be concurrently moved with respect to a third axis orthogonal to both the first and second.

Abstract translation: 描述了二维和三维扫描显微镜成像的运动策略。 物体，样品或样品安装在精密三维平台上。 物体相对于第一轴线和第二轴线与第一轴线相对于切割工具同时移动以切割物体。 随着对象的移动，生成切割部分的图像。 切割工具可以用作用于照射被切割部分的光波导。 光学元件捕获切割和照射物体的图像。 该物体可以进一步相对于与第一和第二两者正交的第三轴同时移动。

公开(公告)号：EP3115766A1

公开(公告)日：2017-01-11

申请号：EP16178713.0

申请日：2016-07-08

Applicant: 3Scan Inc.

Inventor： GOODMAN, Matthew ,  HUFFMAN, Todd ,  DANIEL, Cody

IPC: G01N1/30 ,  G01N1/06 ,  G06K9/00 ,  G06T7/00

CPC classification number: G06T7/0012 ,  G01N1/06 ,  G01N1/30 ,  G06K9/00134 ,  G06K9/0014 ,  G06K9/32 ,  G06T7/33 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: The following concerns a method for co-localization of microscopy or histology stains by the assembly of a virtual image from one or more imaging operations. In particular, the method decreases the time required to obtain multiple labeled antigen or protein histology images of a biological sample. The method includes imaging the tissue as it is sliced by a microtome with a knife edge scanning microscope and spatially aligning the samples by the generated images. The spatial alignment of samples enabled by the method allows a panel of different antigen or protein secondary or functional stains to be compared across different sample slices, thereby allowing concurrent secondary stains of tissues and cells.

Abstract translation: 以下涉及通过从一个或多个成像操作组装虚拟图像来共同定位显微镜或组织学染色的方法。 特别地，该方法减少获得生物样品的多个标记的抗原或蛋白组织学图像所需的时间。 该方法包括使用刀刃扫描显微镜在切片机上对组织进行成像，并通过生成的图像在空间上对准样品。 通过该方法实现的样品的空间比对允许在不同样品切片之间比较不同抗原或蛋白质次级或功能性污渍的一组，从而允许组织和细胞的并发次级染色。

公开(公告)号：EP3076150A1

公开(公告)日：2016-10-05

申请号：EP16162075.2

申请日：2016-03-23

Applicant: 3Scan Inc.

Inventor： DANIEL, Cody ,  GOODMAN, Matthew ,  KOLK, Sean ,  HUFFMAN, Todd

IPC: G01N1/06 ,  G01N1/28

CPC classification number: G01N1/06 ,  G01N1/14 ,  G01N1/28 ,  G01N1/286 ,  G01N1/4077 ,  G01N1/44 ,  G01N35/00009 ,  G01N35/00871 ,  G01N35/04 ,  G01N35/08 ,  G01N2001/066 ,  G01N2001/2873 ,  G01N2001/4088

Abstract: The present disclosure relates to methods and apparatuses for sectioning and imaging tissue or other samples, which are then automatically captured to enable subsequent analysis. The apparatus acts as a slice capture mechanism for serial sectioning microscopy in a fashion which enables subsequent interfacing with secondary microscopic interrogations or for processing with molecular diagnostic tools. The slices are spatially indexed to allow specific slices to be recalled from a library via automated handling techniques described herein.

Abstract translation: 本公开涉及用于对组织或其它样品进行切片和成像的方法和装置，然后将其自动捕获以使得能够进行后续分析。 该装置以连续切片显微镜的切片捕获机制的方式起作用，其能够与二次微观询问或用分子诊断工具进行后处理。 这些切片被空间索引以允许通过本文所述的自动处理技术从库中调用特定切片。