公开(公告)号：US20210080357A1

公开(公告)日：2021-03-18

申请号：US17107852

申请日：2020-11-30

Applicant: 3Scan Inc.

Inventor： Cody Daniel ,  Matthew Goodman ,  Sean Kolk ,  Todd Huffman

IPC: G01N1/06 ,  G01N1/28 ,  G01N1/14 ,  G01N1/40 ,  G01N35/00 ,  G01N35/04 ,  G01N35/08

Abstract: The present disclosure relates to methods and apparatuses for sectioning and imaging tissue or other samples, which are then automatically captured to enable subsequent analysis. The apparatus acts as a slice capture mechanism for serial sectioning microscopy in a fashion which enables subsequent interfacing with secondary microscopic interrogations or for processing with molecular diagnostic tools. The slices are spatially indexed to allow specific slices to be recalled from a library via automated handling techniques described herein.

公开(公告)号：US20210073980A1

公开(公告)日：2021-03-11

申请号：US16950870

申请日：2020-11-17

Applicant: 3Scan Inc.

Inventor： Matthew Goodman ,  Todd Huffman ,  Cody Daniel

IPC: G06T7/00 ,  G01N1/06 ,  G06K9/00 ,  G06T7/33 ,  G06K9/32

Abstract: The following concerns a method for co-localization of microscopy or histology stains by the assembly of a virtual image from one or more imaging operations. In particular, the method decreases the time required to obtain multiple labeled antigen or protein histology images of a biological sample. The method includes imaging the tissue as it is sliced by a microtome with a knife edge scanning microscope and spatially aligning the samples by the generated images. The spatial alignment of samples enabled by the method allows a panel of different antigen or protein secondary or functional stains to be compared across different sample slices, thereby allowing concurrent secondary stains of tissues and cells.