公开(公告)号：JPS63315934A

公开(公告)日：1988-12-23

申请号：JP13823788

申请日：1988-06-02

Applicant: ABBOTT LAB

Inventor： MAIKERU ERU RAMOSU ,  DEIBITSUDO ANSONII YOSUTO ,  DAIAN EMU BEITOSU

IPC: G01N33/62 ,  C12Q1/54 ,  C12Q1/58 ,  G01N21/75 ,  G01N33/66

公开(公告)号：JPS6463397A

公开(公告)日：1989-03-09

申请号：JP13130688

申请日：1988-05-26

Applicant: ABBOTT LAB

Inventor： UIRIAMU EE ABOTSUTO ,  DEIBITSUDO ANSONII YOSUTO ,  RITA SHIYUU BAIRUNE

IPC: C12Q1/32

公开(公告)号：JPS6435372A

公开(公告)日：1989-02-06

申请号：JP17172188

申请日：1988-07-08

Applicant: ABBOTT LAB

Inventor： DEIBITSUDO ANSONII YOSUTO ,  JIYOON KAARO RATSUSERU ,  HIICHIYUN YAN

IPC: G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/551 ,  G01N33/58

Abstract: PURPOSE: To perform high-sensitivity immunoassay over a wide detection range by using a component which specifically recognizes an object to be analyzed and is coupled with colloidal nonmetallic particles as a labeling composition. CONSTITUTION: Colloidal nonmetallic particles are prepared by causing a reaction between SeO2 and ascorbic acid in boiled water. After the pH of the suspension of colloidal selenium particles is adjusted to 7.2, an anti-goat pig antibody against IgG(H+L) is added to the suspension and the suspension is mixed and incubated 10 minutes. Then the supernatant liquid of the suspension is separated by centrifuging the suspension and a bound material is suspended in a TBS casein solution. The suspension of the bound material is mixed with a blood serum sample containing an anti-pig trichina antibody and the mixture is brought into contact with a nitrocellulose strip. As a result, a visually detectable red positive signal is obtained.