公开(公告)号：EP0597034A4

公开(公告)日：1996-04-10

申请号：EP92917936

申请日：1992-07-22

Applicant: ABBOTT LAB

Inventor： DUBLER ROBERT E ,  GROTE JONATHAN ,  KUHN DONNA R

IPC: G01N33/53 ,  C07K16/00 ,  C07K16/44 ,  G01N33/531 ,  G01N33/533 ,  G01N33/542 ,  G01N33/94

CPC classification number: G01N33/533 ,  C07K16/44 ,  G01N33/9486 ,  Y10S436/815

公开(公告)号：CA2030689C

公开(公告)日：2002-12-03

申请号：CA2030689

申请日：1990-11-23

Applicant: ABBOTT LAB

Inventor： GROTE JONATHAN ,  HU HSIANG

IPC: G01N33/53 ,  A61K39/00 ,  C07D405/12 ,  C07K14/00 ,  C07K14/76 ,  C07K14/765 ,  C07K16/00 ,  G01N33/542 ,  G01N33/94 ,  C07D239/60 ,  C09B11/24 ,  G01N33/52 ,  G01N33/533

Abstract: The present invention provides methods and compositions for assaying biological samples, such as human serum, for barbiturates. In one aspect, analogs of barbiturates derivatized with fluorescein and analogs of barbiturates derivatized with immunogenic polypeptides are provided. The fluorescent analogs are employed as tracers in a competitive homogeneous immunoassay, i.e., a fluorescence polarization immunoassay, fox detecting barbiturates. The immunogenic analogs are employed to make anti-barbiturate antiserum of the invention for use in the immunoassay method. Intermediates for preparing the fluorescent and immunogenic analogs are also provided. Further provided are test kits, comprising a fluorescent tracer and an antiserum according to the invention, for analyzing biological samples by fluorescence polarization immunoassay for the presence of a barbiturate. Further, an improvement is provided in immunoassays for analytes in serum, wherein a transfer means, such as a pipette, is used repeatedly to transfer different samples into the assay system. In the improvement, an aqueous solution of specified composition, comprising dimethylsulfoxide and an alkali halide, is used to wash the transfer means between transfers.

公开(公告)号：DE69231060T2

公开(公告)日：2001-02-15

申请号：DE69231060

申请日：1992-07-22

Applicant: ABBOTT LAB

Inventor： DUBLER E ,  GROTE JONATHAN ,  KUHN R

IPC: G01N33/53 ,  C07K16/00 ,  C07K16/44 ,  G01N33/531 ,  G01N33/533 ,  G01N33/542 ,  G01N33/94

Abstract: Disclosed is a substantially optically pure hapten, useful in an immunoassay for dextropropoxyphene and/or nordextropropoxyphene. The hapten corresponds to a specified structural formula (IX). Also disclosed is an immunogen derived from the hapten as well as an antibody raised in response to an immunogen derived from the hapten. Also disclosed is a fluorescent tracer derived from a substantially optically pure compound corresponding to the hapten, the tracer being useful in an immunoassay for dextropropoxyphene and/or nordextropropoxyphene. Also disclosed is an improved immunoassay for determining dextropropoxyphene and/or nordextropropoxyphene in a biological sample involving a step of contacting the sample with antibodies raised in response to the immunogen. Also disclosed is a fluorescence polarization immunoassay (FPIA) for determining dextropropoxyphene and/or nordextropropoxyphene involving a step of contacting the sample with antibodies raised in response to the immunogen, and/or involving a step of contacting the sample with a fluorescent tracer.

公开(公告)号：DE69126915T2

公开(公告)日：1998-02-19

申请号：DE69126915

申请日：1991-05-10

Applicant: ABBOTT LAB

Inventor： ADAMCZYK MACIEJ ,  CANTARERO LUIS A ,  DUBLER ROBERT EDWARD ,  JONAS PATRICK J ,  GROTE JONATHAN ,  NELSON JANE ANN

IPC: A61K39/395 ,  C07D405/04 ,  C07D405/06 ,  C07D405/08 ,  C07D405/10 ,  C07D405/12 ,  G01N33/533 ,  G01N33/536 ,  G01N33/542 ,  G01N33/94 ,  G01N33/532 ,  C07D493/10

Abstract: The present invention is directed to a fluorescence polarization immunoassay for barbiturates, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for preparing them and a reagent kit containing them. The tracers and the immunogens are made from substituted barbiturate compounds. A fluorescein moiety is included in the tracer, while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of polarization retention of plane-polarized light that has been passed through a sample containing antiserum and tracer.

公开(公告)号：CA2191613A1

公开(公告)日：1996-02-01

申请号：CA2191613

申请日：1995-06-16

Applicant: ABBOTT LAB

Inventor： MURRAY ADRIAN JOHN ,  GROTE JONATHAN ,  ST JOHN SHERRI J ,  THUT DIETHILD

IPC: G01N33/531 ,  C09B15/00 ,  C09B67/44 ,  C09K11/07 ,  G01N33/53 ,  G01N33/532 ,  G01N33/533 ,  G01N33/58 ,  G01N33/50

Abstract: The present invention provides a method for generating an enhanced chemiluminescent signal from an acridinium sulfonamide compound, the method comprising the step of contacting the acridinium sulfonamide compound with a trigger solution in the presence of an enhancer to obtain a chemiluminescent signal which is stronger than would otherwise be generated in the absence of said enhancer. The enhancer comprises at least one member selected from the group consisting of nonionic surfactants, zwitterionic surfactants, and anionic surfactants.

公开(公告)号：AU7708191A

公开(公告)日：1991-08-29

申请号：AU7708191

申请日：1991-05-15

Applicant: ABBOTT LAB

Inventor： ADAMCZYK MACIEJ BOGDAN ,  DUBLER ROBERT EDWARD ,  JONAS PATRICK FRANCIS ,  CANTARERO LUIS AUGUSTO ,  GROTE JONATHAN ,  NELSON JANE ANN

IPC: A61K39/395 ,  C07D405/04 ,  C07D405/06 ,  C07D405/08 ,  C07D405/10 ,  C07D405/12 ,  G01N33/533 ,  G01N33/536 ,  G01N33/542 ,  G01N33/94 ,  C07D493/10

Abstract: The present invention is directed to a fluorescence polarization immunoassay for barbiturates, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for preparing them and a reagent kit containing them. The tracers and the immunogens are made from substituted barbiturate compounds. A fluorescein moiety is included in the tracer, while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of polarization retention of plane-polarized light that has been passed through a sample containing antiserum and tracer.

公开(公告)号：ES2106040T3

公开(公告)日：1997-11-01

申请号：ES91107624

申请日：1991-05-10

Applicant: ABBOTT LAB

Inventor： ADAMCZYK MACIEJ ,  CANTARERO LUIS A ,  DUBLER ROBERT EDWARD ,  JONAS PATRICK J ,  GROTE JONATHAN ,  NELSON JANE ANN

IPC: A61K39/395 ,  C07D405/04 ,  C07D405/06 ,  C07D405/08 ,  C07D405/10 ,  C07D405/12 ,  G01N33/533 ,  G01N33/536 ,  G01N33/542 ,  G01N33/94 ,  G01N33/532 ,  C07D493/10

Abstract: The present invention is directed to a fluorescence polarization immunoassay for barbiturates, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for preparing them and a reagent kit containing them. The tracers and the immunogens are made from substituted barbiturate compounds. A fluorescein moiety is included in the tracer, while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of polarization retention of plane-polarized light that has been passed through a sample containing antiserum and tracer.

公开(公告)号：AT155893T

公开(公告)日：1997-08-15

申请号：AT91107624

申请日：1991-05-10

Applicant: ABBOTT LAB

Inventor： ADAMCZYK MACIEJ ,  CANTARERO LUIS A ,  DUBLER ROBERT EDWARD ,  JONAS PATRICK J ,  GROTE JONATHAN ,  NELSON JANE ANN

IPC: A61K39/395 ,  C07D405/04 ,  C07D405/06 ,  C07D405/08 ,  C07D405/10 ,  C07D405/12 ,  G01N33/533 ,  G01N33/536 ,  G01N33/542 ,  G01N33/94 ,  G01N33/532 ,  C07D493/10 ,  C07D311/00

Abstract: The present invention is directed to a fluorescence polarization immunoassay for barbiturates, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for preparing them and a reagent kit containing them. The tracers and the immunogens are made from substituted barbiturate compounds. A fluorescein moiety is included in the tracer, while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of polarization retention of plane-polarized light that has been passed through a sample containing antiserum and tracer.

公开(公告)号：AU644871B2

公开(公告)日：1993-12-23

申请号：AU7708191

申请日：1991-05-15

Applicant: ABBOTT LAB

Inventor： ADAMCZYK MACIEJ BOGDAN ,  DUBLER ROBERT EDWARD ,  JONAS PATRICK FRANCIS ,  CANTARERO LUIS AUGUSTO ,  GROTE JONATHAN ,  NELSON JANE ANN

IPC: A61K39/395 ,  C07D405/04 ,  C07D405/06 ,  C07D405/08 ,  C07D405/10 ,  C07D405/12 ,  G01N33/533 ,  G01N33/536 ,  G01N33/542 ,  G01N33/94 ,  C07D493/10

Abstract: The present invention is directed to a fluorescence polarization immunoassay for barbiturates, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for preparing them and a reagent kit containing them. The tracers and the immunogens are made from substituted barbiturate compounds. A fluorescein moiety is included in the tracer, while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of polarization retention of plane-polarized light that has been passed through a sample containing antiserum and tracer.

公开(公告)号：CA2030689A1

公开(公告)日：1991-05-28

申请号：CA2030689

申请日：1990-11-23

Applicant: ABBOTT LAB

Inventor： GROTE JONATHAN ,  HU HSIANG

IPC: G01N33/53 ,  A61K39/00 ,  C07D405/12 ,  C07K14/00 ,  C07K14/76 ,  C07K14/765 ,  C07K16/00 ,  G01N33/542 ,  G01N33/94 ,  G01N33/533 ,  G01N33/52

Abstract: The present invention provides methods and compositions for assaying biological samples, such as human serum, for barbiturates. In one aspect, analogs of barbiturates derivatized with fluorescein and analogs of barbiturates derivatized with immunogenic polypeptides are provided. The fluorescent analogs are employed as tracers in a competitive homogeneous immunoassay, i.e., a fluorescence polarization immunoassay, fox detecting barbiturates. The immunogenic analogs are employed to make anti-barbiturate antiserum of the invention for use in the immunoassay method. Intermediates for preparing the fluorescent and immunogenic analogs are also provided. Further provided are test kits, comprising a fluorescent tracer and an antiserum according to the invention, for analyzing biological samples by fluorescence polarization immunoassay for the presence of a barbiturate. Further, an improvement is provided in immunoassays for analytes in serum, wherein a transfer means, such as a pipette, is used repeatedly to transfer different samples into the assay system. In the improvement, an aqueous solution of specified composition, comprising dimethylsulfoxide and an alkali halide, is used to wash the transfer means between transfers.