公开(公告)号：SE8000843L

公开(公告)日：1980-08-06

申请号：SE8000843

申请日：1980-02-01

Applicant: ABBOTT LAB

Inventor： VOSS H F ,  PLATTNER J ,  HERRIN T R

IPC: C07F9/40 ,  C12Q1/46 ,  G01N33/532 ,  G01N33/542 ,  G01N33/54

Abstract: The present invention encompasses a method for determining ligands in test samples comprising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount of ligand in the test sample, said binding protein inactivating the irreversible enzyme inhibitor when bound to the ligand analog portion of the conjugate; intermixing an enzyme which is irreversibly inhibited by the ligand analog-irreversible enzyme inhibitor conjugate unbound by the binding protein; and intermixing substrate to the enzyme and monitoring the enzyme substrate reaction. The invention also includes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in practicing the method. Methods and reagents of the present are particularly useful in determining drugs, hormones, and the like in biological fluids.

公开(公告)号：SE448259B

公开(公告)日：1987-02-02

申请号：SE8000843

申请日：1980-02-01

Applicant: ABBOTT LAB

Inventor： VOSS H F ,  PLATTNER J ,  HERRIN T R

IPC: C07F9/40 ,  C12Q1/46 ,  G01N33/532 ,  G01N33/542

Abstract: The present invention encompasses a method for determining ligands in test samples comprising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount of ligand in the test sample, said binding protein inactivating the irreversible enzyme inhibitor when bound to the ligand analog portion of the conjugate; intermixing an enzyme which is irreversibly inhibited by the ligand analog-irreversible enzyme inhibitor conjugate unbound by the binding protein; and intermixing substrate to the enzyme and monitoring the enzyme substrate reaction. The invention also includes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in practicing the method. Methods and reagents of the present are particularly useful in determining drugs, hormones, and the like in biological fluids.

公开(公告)号：NZ209983A

公开(公告)日：1988-01-08

申请号：NZ20998384

申请日：1984-10-25

Applicant: ABBOTT LAB

Inventor： FLENTGE C A ,  HERRIN T R

IPC: C12N9/99 ,  A61K31/66 ,  A61P43/00 ,  C07F9/40 ,  C07F9/553 ,  C07F9/6512 ,  C07D223/18 ,  C07D239/62 ,  C07F9/65

Abstract: Carbamazepine-thiophosphonate conjugates for use as enzyme inhibitors having the formula: wherein Z is a biologically compatible counter ion, n is an integer from 2 to 6 and R and R1 are each lower alkyl.