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公开(公告)号：JPH01153962A
公开(公告)日：1989-06-16
申请号：JP26699988
申请日：1988-10-20
Applicant: ABBOTT LAB
Inventor： BIRII JIEI GURIIN , UIRIAMU AARU GUROSUKOTSUPU , ROBAATO KOWAARU , TOOMASU ARAN RAASON , POORU BII WAANKU JIYUNIA
Abstract: PURPOSE: To directly measure thyroid gland hormone in serum or plasma by treating a sample by furosemide, substituting triopfpyjutpmomr (T3 ) and thyroxine (T4 ) from binding protein, and measuring the amount of substituted T3 and T4 . CONSTITUTION: A sample is treated by furosemide, T3 and T4 are substituted from a binding protein, and the amount of substituted T3 and T4 is measured. The amount of the substituted T3 and T4 can be measured by using either various kinds of thyroid gland hormone assay reagents and methods. Also, the amount of forosemide to be used can be calculated as a function of the size of a sample to substitute essentially all T3 and T4 . Also, either fluorescence, absorption, fluorescent polarization, chemical lunescence, or radioactive means can be used as a detection method.
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