公开(公告)号：AU7600994A

公开(公告)日：1995-03-21

申请号：AU7600994

申请日：1994-08-22

Applicant: ABBOTT LAB

Inventor： GRANADOS EDWARD N ,  CHOW HERBERT S ,  HEIMAN DANIEL F ,  THALER MARIA L

IPC: C12N5/06 ,  G01N33/50 ,  G01N33/543 ,  G01N33/554 ,  G01N33/556 ,  G01N33/569

公开(公告)号：CA2167931A1

公开(公告)日：1995-03-02

申请号：CA2167931

申请日：1994-08-22

Applicant: ABBOTT LAB

Inventor： GRANADOS EDWARD N ,  CHOW HERBERT S ,  HEIMAN DANIEL F ,  THALER MARIA L

IPC: C12N5/06 ,  G01N33/50 ,  G01N33/543 ,  G01N33/554 ,  G01N33/556 ,  G01N33/569 ,  G01N33/557 ,  G01N33/564

Abstract: A method is provided for the preparation of semisolid particles or cells that have been chemically derivatized resulting in hydrazide functionalities covalently incorporated onto cell surface membrane molecules of the semisolid particles or cells, as well as a method for determining the levels of hydrazide groups on the particles or cells. The method involves the preparation and analysis of red blood cells chemically derivatized in such a manner that hydrazide functionalities have been covalently incorporated onto the cell membrane molecules, the preparation of hydrazine derivatized cells and the use thereof of the novel cells in agglutination reactions to promote the attachment of antigens and antibodies to the hydrazine derivatized cells without loss of antibody activity or the blocking of the epitope of interest.