公开(公告)号：EP1266219A2

公开(公告)日：2002-12-18

申请号：EP01922545.7

申请日：2001-03-21

Applicant: ABBOTT LABORATORIES

Inventor： TRUMBULL, Jonathan, D. ,  BERTRAND, Daniel, C. ,  BRIGGS, Clark, A. ,  MCKENNA, David, G. ,  MASLANA, Eugene, S. ,  BLANCHARD, David, P. ,  PAN, Jeffrey, Y. ,  BOJAN, Peter, M. ,  NEMCEK, Thomas, A.

IPC: G01N33/487 ,  G01N35/02 ,  G01N33/50

CPC classification number: G01N35/028

Abstract: A method and apparatus for running a plurality of tests concurrently to obtain data relating to the electrophysiological properties of receptors and channels in biological membranes of test subjects, such as, for example, Xenopus oocytes. The invention further provides software for controlling, acquiring, and recording data relating to electrophysiological properties of receptors and channels in biological membranes of test subjects, such as, for example, oocytes. This invention increases the throughput rate for experiments and assays employing receptors and ion channels expressed in biological membranes of test subjects, such as, for example, oocytes. In the case of an oocyte, these receptors and channels may be natively expressed (endogenous), may be placed into the oocyte (exogenous), or may be expressed from other RNA or DNA previously placed into the oocyte (exogenous). The invention provides a means for a sole researcher to operate a plurality of electrophysiological test stations in the time and space conventionally required by a single electrophysiological test station. The invention automates these stations and provides a means for a sole individual to perform large sets of experiments that would be physically and mentally exhausting in the absence of this invention. In addition, this invention provides efficient database and data analysis software integrated with the data acquisition software, thereby increasing the user's data-handling productivity to keep pace with the augmented data generation capacity.

Abstract translation: 用于同时运行多个测试以获得与测试受试者（例如非洲爪蟾卵母细胞）的生物膜中的受体和通道的电生理学特性有关的数据的方法和装置。 本发明进一步提供了用于控制，获取和记录与受试者（例如卵母细胞）的生物膜中的受体和通道的电生理学特性有关的数据的软件。 本发明提高了使用受试者（例如卵母细胞）的生物膜中表达的受体和离子通道的实验和测定的通过率。 在卵母细胞的情况下，这些受体和通道可以是天然表达的（内源性的），可以被置于卵母细胞中（外源性的），或者可以从先前置于卵母细胞（外源性）的其他RNA或DNA中表达。 本发明提供了一种用于唯一研究者在单个电生理测试站通常所需的时间和空间中操作多个电生理学测试站的手段。 本发明使这些站自动化，并为单个人提供了一种手段，用于执行大量的实验，在没有本发明的情况下，这些实验在身体上和精神上都会耗尽。 此外，本发明提供了与数据采集软件集成的高效数据库和数据分析软件，从而提高了用户的数据处理生产率，以跟上增强的数据生成能力。