公开(公告)号：EP0424634A2

公开(公告)日：1991-05-02

申请号：EP90116380.8

申请日：1990-08-27

Applicant: ABBOTT LABORATORIES

Inventor： Khalil, Omar S. ,  Zurek, Thomas F. ,  Genger, Kevin R. ,  Pepe, Curtis J. ,  Jou, Yi-Her ,  Cotter, Stephen M.

IPC: G01N33/52 ,  G01N33/58

CPC classification number: G01N33/54346 ,  G01N21/76 ,  G01N33/521 ,  G01N33/525 ,  G01N33/54366 ,  G01N33/54386 ,  G01N33/5764

Abstract: Apparatus and method for performing a chemiluminescende assay involving the immobilization of a chemiluminescent reaction complex to a solid, porous element. The solid, porous element is preferably treated to provide an immobilizing interaction with the chemiluminescent reaction complex wherein the chemiluminescent reaction complex is thereby immobilized to the solid, porous element. The activating and reading of the chemiluminescent reaction are separately performed by evenly distributing a concentrated chemiluminescent activating solution to form a puddle on the surface of the porous element to which the chemiluminescent reaction complex is immobilized.

Abstract translation: 用于进行涉及将化学发光反应复合物固定在固体多孔元件上的化学发光测定的装置和方法。 优选处理固体多孔元件以提供与化学发光反应复合物的固定相互作用，其中化学发光反应复合物由此固定在固体多孔元件上。 化学发光反应的激活和读取分别通过均匀分布浓缩的化学发光活化溶液在化学发光反应复合物固定的多孔元件的表面上形成一个水坑。

公开(公告)号：EP0424634A3

公开(公告)日：1992-03-04

申请号：EP90116380.8

申请日：1990-08-27

Applicant: ABBOTT LABORATORIES

Inventor： Khalil, Omar S. ,  Zurek, Thomas F. ,  Genger, Kevin R. ,  Pepe, Curtis J. ,  Jou, Yi-Her ,  Cotter, Stephen M.

IPC: G01N33/52 ,  G01N33/58

CPC classification number: G01N33/54346 ,  G01N21/76 ,  G01N33/521 ,  G01N33/525 ,  G01N33/54366 ,  G01N33/54386 ,  G01N33/5764

Abstract: Apparatus and method for performing a chemiluminescende assay involving the immobilization of a chemiluminescent reaction complex to a solid, porous element. The solid, porous element is preferably treated to provide an immobilizing interaction with the chemiluminescent reaction complex wherein the chemiluminescent reaction complex is thereby immobilized to the solid, porous element. The activating and reading of the chemiluminescent reaction are separately performed by evenly distributing a concentrated chemiluminescent activating solution to form a puddle on the surface of the porous element to which the chemiluminescent reaction complex is immobilized.

公开(公告)号：EP0424634B1

公开(公告)日：1995-06-14

申请号：EP90116380.8

申请日：1990-08-27

Applicant: ABBOTT LABORATORIES

Inventor： Khalil, Omar S. ,  Zurek, Thomas F. ,  Genger, Kevin R. ,  Pepe, Curtis J. ,  Jou, Yi-Her ,  Cotter, Stephen M.

IPC: G01N33/52 ,  G01N33/58

CPC classification number: G01N33/54346 ,  G01N21/76 ,  G01N33/521 ,  G01N33/525 ,  G01N33/54366 ,  G01N33/54386 ,  G01N33/5764

Abstract: Apparatus and method for performing a chemiluminescende assay involving the immobilization of a chemiluminescent reaction complex to a solid, porous element. The solid, porous element is preferably treated to provide an immobilizing interaction with the chemiluminescent reaction complex wherein the chemiluminescent reaction complex is thereby immobilized to the solid, porous element. The activating and reading of the chemiluminescent reaction are separately performed by evenly distributing a concentrated chemiluminescent activating solution to form a puddle on the surface of the porous element to which the chemiluminescent reaction complex is immobilized.

公开(公告)号：EP0341438A3

公开(公告)日：1990-11-28

申请号：EP89106444.6

申请日：1989-04-11

Applicant: ABBOTT LABORATORIES

Inventor： Hollar, Robert C. ,  Pepe, Curtis J. ,  Genger, Kevin R.

IPC: G01F23/14 ,  G01N35/06

CPC classification number: G01N35/109 ,  G01F23/14 ,  G01N2035/1018 ,  G01N2035/1025

Abstract: For use in testing a large volume of blood samples, portions of which are to be aspirated and then dispensed into one or more reaction wells, a pneumatic sensing system is characterized by an air pressure/vacuum source which is automatically coupled to a disposable pipette tip when the tip is picked up by a programmed gantry to provide a low pressure air flow through the pipette tip as it approaches the surface of the sample to be aspirated. A back pressure developed in the system as the pipette tip closely approaches and contacts the surface of the sample is detected by a pressure transducer which feeds the detected data to the gantry controller which instantaneously raises the pipette tip for purging and then moves it downwardly into the sample a distance equal to the sensed surface level plus a constant distance appropriate for the amount of the sample to be aspirated. Verification of the integrity of the system during both aspiration and dispensing of the samples is provided by the pressure transducer and the controller with a display screen of the controller providing a visual indicator.

公开(公告)号：EP0341438A2

公开(公告)日：1989-11-15

申请号：EP89106444.6

申请日：1989-04-11

Applicant: ABBOTT LABORATORIES

Inventor： Hollar, Robert C. ,  Pepe, Curtis J. ,  Genger, Kevin R.

IPC: G01F23/14 ,  G01N35/06

CPC classification number: G01N35/109 ,  G01F23/14 ,  G01N2035/1018 ,  G01N2035/1025

Abstract: For use in testing a large volume of blood samples, portions of which are to be aspirated and then dispensed into one or more reaction wells, a pneumatic sensing system is characterized by an air pressure/vacuum source which is automatically coupled to a disposable pipette tip when the tip is picked up by a programmed gantry to provide a low pressure air flow through the pipette tip as it approaches the surface of the sample to be aspirated. A back pressure developed in the system as the pipette tip closely approaches and contacts the surface of the sample is detected by a pressure transducer which feeds the detected data to the gantry controller which instantaneously raises the pipette tip for purging and then moves it downwardly into the sample a distance equal to the sensed surface level plus a constant distance appropriate for the amount of the sample to be aspirated. Verification of the integrity of the system during both aspiration and dispensing of the samples is provided by the pressure transducer and the controller with a display screen of the controller providing a visual indicator.

Abstract translation: 为了用于测试大量的血液样品，其中的一部分被吸入然后分配到一个或多个反应孔中，气动感测系统的特征在于气压/真空源，其自动地联接到一次性吸头 当尖端被编程的台架拾取以提供通过移液管尖端的低压空气流，因为其接近待吸取的样品的表面。 当移液管尖端紧密接近并接触样品表面时，在系统中产生的背压由压力传感器检测，压力传感器将检测到的数据馈送到龙门架控制器，立式升高移液管吸头用于清洗，然后向下移动到 样品的距离等于感测到的表面水平加上适合抽吸样本量的恒定距离。 通过压力传感器和控制器提供抽吸和分配样品期间系统完整性的验证，控制器具有控制器的显示屏幕，其提供视觉指示器。