公开(公告)号：WO1992008979A1

公开(公告)日：1992-05-29

申请号：PCT/US1991008360

申请日：1991-11-08

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  HU, Roger, C. ,  ROBERTSON, Eugene, F. ,  MONTGOMERY, Deborah, M. ,  PETERSON, Bryan, C. ,  ALI, Akhtar ,  MACHOWIAK, James, P. ,  GUIDINGER, Peggy, L. ,  KHALIL, Omar, S.

IPC: G01N33/536

CPC classification number: G01N33/54306 ,  G01N33/582

Abstract: A chemiluminescent signal amplification method in which the desired reaction signal is amplified by use of a probe reagent which contains an enhancer compound such as a hapten and by the use of a conjugate which contains a chemiluminescent signal generating compound. Kits for performing such an amplified chemiluminescent assay are also provided.

Abstract translation: 一种化学发光信号扩增方法，其中通过使用含有增强子化合物如半抗原的探针试剂和通过使用包含化学发光信号产生化合物的缀合物来扩增所需反应信号。 还提供了用于进行这种扩增化学发光测定的试剂盒。

公开(公告)号：WO1994010258A1

公开(公告)日：1994-05-11

申请号：PCT/US1993010258

申请日：1993-10-26

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  WANG, Nai-Yi ,  HU, Roger, C.

IPC: C09K03/00

CPC classification number: G01N33/582 ,  C07F7/1804 ,  C07F9/65512 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q2334/00 ,  G01N33/533 ,  Y10S435/968

Abstract: Chemiluminescent electron-rich aryl-substituted 1,2-dioxetane compounds are disclosed in which the aryl group is poly-substituted with suitable electron-donating groups such that the light-emitting pattern of the molecule results in a very high luminescent count, thus providing for a sensitive and precise assay for haptens, analytes, polynucleotides and the like. These substituted aryl-containing 1,2-dioxetane compounds can be used as direct labels in an immunoassay or when derivatized with an appropriate leaving group, can be used as a substrate for an enzyme immunoassay. The unusual chemiluminescence of the compounds allows the timing of the luminescent reaction to be exactly controlled.

Abstract translation: 公开了化学发光富电子的芳基取代的1,2-二氧杂环丁烷化合物，其中芳基被合适的给电子基团多取代，使得分子的发光图案导致非常高的发光计数，从而提供 对于半抗原，分析物，多核苷酸等的敏感和精确的测定。 这些取代的含芳基的1,2-二氧杂环丁烷化合物可以在免疫测定中用作直接标记，或者当用合适的离去基团衍生化时，可用作酶免疫测定的底物。 化合物的不寻常的化学发光使得准确控制发光反应的时间。

公开(公告)号：EP0556331A1

公开(公告)日：1993-08-25

申请号：EP92901340.0

申请日：1991-11-08

Applicant: ABBOTT LABORATORIES

Inventor： HU, Roger, C. ,  ROBERTSON, Eugene, F. ,  MONTGOMERY, Deborah, M. ,  PETERSON, Bryan, C. ,  ALI, Akhtar ,  MACHOWIAK, James, P. ,  GUIDINGER, Peggy, L. ,  KHALIL, Omar, S.

IPC: G01N33

CPC classification number: G01N33/54306 ,  G01N33/582

Abstract: Procédé d'amplification de signal chimioluminescent, selon lequel le signal de réaction désiré est amplifié au moyen d'un réactif agissant comme une sonde qui contient un composé de renforcement tel qu'un haptène, et au moyen d'un produit conjugué contenant un composé générateur de signal chimioluminescent. On décrit aussi des kits servant à effectuer de telles analyses chimioluminescentes amplifiées.

公开(公告)号：EP0666891B1

公开(公告)日：2002-01-16

申请号：EP93925057.7

申请日：1993-10-26

Applicant: ABBOTT LABORATORIES

Inventor： WANG, Nai-Yi ,  HU, Roger, C.

IPC: C09K3/00 ,  C12Q1/00 ,  C12Q1/70 ,  C12Q1/68 ,  C07D327/02 ,  C07D321/00 ,  C07D305/00 ,  C07F9/06

CPC classification number: G01N33/582 ,  C07F7/1856 ,  C07F9/65512 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q2334/00 ,  G01N33/533 ,  Y10S435/968

Abstract: Chemiluminescent electron-rich aryl-substituted 1,2-dioxetane compounds are disclosed in which the aryl group is poly-substituted with suitable electron-donating groups such that the light-emitting pattern of the molecule results in a very high luminescent count, thus providing for a sensitive and precise assay for haptens, analytes, polynucleotides and the like. These substituted aryl-containing 1,2-dioxetane compounds can be used as direct labels in an immunoassay or when derivatized with an appropriate leaving group, can be used as a substrate for an enzyme immunoassay. The unusual chemiluminescence of the compounds allows the timing of the luminescent reaction to be exactly controlled.

公开(公告)号：EP0666891A1

公开(公告)日：1995-08-16

申请号：EP93925057.0

申请日：1993-10-26

Applicant: ABBOTT LABORATORIES

Inventor： WANG, Nai-Yi ,  HU, Roger, C.

IPC: C07D321 ,  C07D493 ,  C07F7 ,  C07F9 ,  C12Q1 ,  G01N33

CPC classification number: G01N33/582 ,  C07F7/1856 ,  C07F9/65512 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q2334/00 ,  G01N33/533 ,  Y10S435/968

Abstract: Chemiluminescent electron-rich aryl-substituted 1,2-dioxetane compounds are disclosed in which the aryl group is poly-substituted with suitable electron-donating groups such that the light-emitting pattern of the molecule results in a very high luminescent count, thus providing for a sensitive and precise assay for haptens, analytes, polynucleotides and the like. These substituted aryl-containing 1,2-dioxetane compounds can be used as direct labels in an immunoassay or when derivatized with an appropriate leaving group, can be used as a substrate for an enzyme immunoassay. The unusual chemiluminescence of the compounds allows the timing of the luminescent reaction to be exactly controlled.