公开(公告)号：WO1997046881A1

公开(公告)日：1997-12-11

申请号：PCT/US1997009429

申请日：1997-06-03

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  POPE, Mark, R. ,  TARCHA, Peter, J. ,  MEES, David, R. ,  JOSEPH, Mary, K. ,  PRY, Terry, A. ,  PUTMAN, C., Brent ,  SUBOTICH, Daniel, T.

IPC: G01N33/543

CPC classification number: G01N33/54393 ,  G01N33/54353 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S436/81 ,  Y10S436/823

Abstract: A method for increasing the binding activity of specific binding members bound to a solid phase material, e.g., a particle, that has been sterically stabilized. This increase in binding activity is brought about by degrading a steric stabilizer on the surface of the solid phase material. The method involves both immobilizing a specific binding member on the surface of a solid phase material and degrading a steric stabilizer on the surface of that solid phase material. In the preferred embodiment, the method involves the immobilization of a specific binding member on the surface of the sterically stabilized solid phase material, with subsequent degradation of the steric stabilizer.

Abstract translation: 用于增加与已经空间稳定的固相物质例如颗粒结合的特异性结合成员的结合活性的方法。 结合活性的这种增加是通过降解固相材料表面上的空间稳定剂引起的。 该方法包括将特定的结合构件固定在固相材料的表面上并降解固相材料表面上的空间稳定剂。 在优选的实施方案中，该方法包括将特定结合成分固定在空间稳定的固相材料的表面上，随后空间稳定剂的降解。

公开(公告)号：WO1993015097A1

公开(公告)日：1993-08-05

申请号：PCT/US1993000988

申请日：1993-01-28

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  POPE, Mark, R. ,  BIENIARZ, Christopher

IPC: C07H15/24

CPC classification number: C07H15/24 ,  C12Q1/34 ,  C12Q2334/00 ,  G01N2333/924 ,  Y10S436/80 ,  Y10S436/808

Abstract: Novel pyrene-trisulfonate derivatives and the use thereof in a method for the determination of glycohydrolitic enzyme activity are provided. The method comprises the steps of (a) forming a test solution comprising a test sample containing the glycohydrolitic enzyme and a pyrene-trisulfonate derivative of the present invention, wherein the derivative is hydrolyzed by the glycohydrolytic enzyme to result in the formation of free 8-hydroxy-1,3,6-pyrene trisulfonate as a function of, and which can be correlated to, the amount of the glycohydrolytic enzyme present in the test sample, and (b) measuring and correlating either the intensity of fluorescence, or the optical density, of the test solution to the presence or amount of the glycohydrolytic enzyme in the test sample. A preferred pyrene-trisulfonate derivative is pyrene-(1,3,6-trisulfonic acid)-8-β-D-glucuronide for the determination of β-D-glucuronidase for the diagnosis of periodontal disease.

公开(公告)号：EP1011490B1

公开(公告)日：2004-09-29

申请号：EP98911910.2

申请日：1998-03-20

Applicant: Abbott Laboratories

Inventor： LOOMIS, Neil, W. ,  BOJAN, Peter, M. ,  HENNING, Timothy, P. ,  POPE, Mark, R. ,  MUETTERTIES, Andrew, J.

IPC: A61B18/18 ,  A61B18/20

CPC classification number: A61B18/20 ,  A61B5/14514 ,  A61B5/150022 ,  A61B5/15136 ,  A61B2017/00765 ,  A61B2018/00636 ,  A61B2018/1807 ,  A61M2037/0007

Abstract: A method for increasing the permeability of the stratum corneum by means of a source of light, preferably a laser, more preferably a pulsed laser. By increasing the permeability of the stratum corneum, access to the interstitial fluid is achieved, thereby enabling measurement of analytes in the interstitial fluid. In one aspect, the method comprises the steps of (a) providing a source of light having a wavelength of from about 930 nm to about 1040 nm; and (b) exposing a region of the stratum corneum of the patient to said source of light for a period of time sufficient to form an opening in the stratum corneum. Preferably, exposure of the region of the stratum corneum to the source of light is ceased when an amount of interstitial fluid fills the opening in the stratum corneum, which amount is sufficient to cause the scatter intensity of the light reflected from the surface of the interstitial fluid occupying the opening in the stratum corneum to differ from the scatter intensity of the light reflected from a region of the stratum corneum that is substantially free of interstitial fluid. The invention also involves an apparatus for carrying out the foregoing method.

公开(公告)号：EP1056396A1

公开(公告)日：2000-12-06

申请号：EP99907051.9

申请日：1999-02-16

Applicant: ABBOTT LABORATORIES

Inventor： BOJAN, Peter, M. ,  HENNING, Timothy, P. ,  LOOMIS, Neil, W. ,  POPE, Mark, R. ,  EPPSTEIN, Jonathan

IPC: A61B10/00 ,  A61K41/00 ,  A61B5/103

CPC classification number: A61B5/14865 ,  A61B5/14514 ,  A61B5/1486 ,  A61B5/150022 ,  A61B5/150343 ,  A61B5/150358 ,  A61B5/150755 ,  A61B5/157 ,  A61B5/441 ,  A61B10/0045 ,  A61B18/20 ,  A61B2010/008 ,  A61B2017/00765 ,  A61B2018/1807 ,  A61B2562/0295 ,  Y10T436/2575

Abstract: An article (10) capable of both collecting interstitial fluid and detecting an analyte in that fluid and a method for use of that article (10). Preferably, the article (10) is also capable of measuring the amount of analyte in the interstitial fluid. The article (10) can be used in conjunction with a meter that contains an appropriate detection element for determining the amount of analyte in the interstitial fluid. In one preferred embodiment, the article (10) is a multiple-layer element comprising: (1) a layer (15) that is capable of being placed in contact with the skin of a patient; (2) a layer (16) that is coated over the skin-contacting layer (15); (3) a layer (18), substantially coplanar with the overcoat layer (16), that is capable of transporting interstitial fluid by means of chemically aided wicking; (4) a layer (20), overlying the interstitial fluid transporting layer (18), that is capable of being placed in contact with a meter (28), said layer having an opening (24) therein through which light can be transmitted; (5) a layer (28), disposed on the surface of the meter-contacting layer (20) that faces the skin-contacting layer (15), that is capable of detecting the presence of analyte or measuring the amount of analyte in the fluid. In order to use the multiple-layer (18) element (10), light from a source of light is transmitted through the opening (24) in the multiple-layer material to be absorbed at a light-absorbing target (22) on the skin-contacting layer (15). This light transfers energy to the target (22), and this transferred energy causes an opening to form in the skin-contacting layer (15) and an opening to form in the stratum corneum. Interstitial fluid exudes from the opening in the stratum corneum and contacts the interstitial fluid transporting layer (18). The interstitial fluid then moves along or through the interstitial fluid transporting layer (18) to the detecting layer (28).

公开(公告)号：EP1079737A1

公开(公告)日：2001-03-07

申请号：EP99923149.1

申请日：1999-05-17

Applicant: ABBOTT LABORATORIES

Inventor： SHAIN, Eric, B. ,  POPE, Mark, R. ,  PEZZANITI, Joseph, L.

IPC: A61B17/00

CPC classification number: A61B18/203 ,  A61B5/150022 ,  A61B5/15136 ,  A61B10/0045 ,  A61B18/20 ,  A61B2017/00057 ,  A61B2017/00765 ,  A61B2018/00452 ,  A61B2018/00636

Abstract: A method for focusing light comprising the steps of: (1) projecting at least one pulse of light onto a surface of the skin of a patient; (2) collecting at least a portion of the light that is reflected from the skin of the patient; (3) projecting the collected, reflected light onto a detector; and (10) adjusting the projection of the pulsed light onto the surface of the skin of the patient in such a manner that the signal projected onto the detector is optimized. When the pulsed light is properly focused, e.g., when it is characterized by the best focus, it can be used to provide energy to form an opening in the skin of the patient. When more than one pulse of light is required to form an opening in the skin of the patient, aligning the light prior to each pulse will improve the efficiency of formation of the opening.

公开(公告)号：EP0923735A1

公开(公告)日：1999-06-23

申请号：EP97926857.0

申请日：1997-06-03

Applicant: Abbott Laboratories

Inventor： POPE, Mark, R. ,  TARCHA, Peter, J. ,  MEES, David, R. ,  JOSEPH, Mary, K. ,  PRY, Terry, A. ,  PUTMAN, C., Brent ,  SUBOTICH, Daniel, T.

IPC: G01N33

CPC classification number: G01N33/54393 ,  G01N33/54353 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S436/81 ,  Y10S436/823

Abstract: A method for increasing the binding activity of specific binding members bound to a solid phase material, e.g., a particle, that has been sterically stabilized. This increase in binding activity is brought about by degrading a steric stabilizer on the surface of the solid phase material. The method involves both immobilizing a specific binding member on the surface of a solid phase material and degrading a steric stabilizer on the surface of that solid phase material. In the preferred embodiment, the method involves the immobilization of a specific binding member on the surface of the sterically stabilized solid phase material, with subsequent degradation of the steric stabilizer.

公开(公告)号：EP1056396B1

公开(公告)日：2005-11-09

申请号：EP99907051.9

申请日：1999-02-16

Applicant: ABBOTT LABORATORIES

Inventor： BOJAN, Peter, M. ,  HENNING, Timothy, P. ,  LOOMIS, Neil, W. ,  POPE, Mark, R. ,  EPPSTEIN, Jonathan

IPC: A61B10/00 ,  A61K41/00 ,  A61B5/103

CPC classification number: A61B5/14865 ,  A61B5/14514 ,  A61B5/1486 ,  A61B5/150022 ,  A61B5/150343 ,  A61B5/150358 ,  A61B5/150755 ,  A61B5/157 ,  A61B5/441 ,  A61B10/0045 ,  A61B18/20 ,  A61B2010/008 ,  A61B2017/00765 ,  A61B2018/1807 ,  A61B2562/0295 ,  Y10T436/2575

Abstract: An article (10) capable of both collecting interstitial fluid and detecting an analyte in that fluid and a method for use of that article (10). Preferably, the article (10) is also capable of measuring the amount of analyte in the interstitial fluid. The article (10) can be used in conjunction with a meter that contains an appropriate detection element for determining the amount of analyte in the interstitial fluid. In one preferred embodiment, the article (10) is a multiple-layer element comprising: (1) a layer (15) that is capable of being placed in contact with the skin of a patient; (2) a layer (16) that is coated over the skin-contacting layer (15); (3) a layer (18), substantially coplanar with the overcoat layer (16), that is capable of transporting interstitial fluid by means of chemically aided wicking; (4) a layer (20), overlying the interstitial fluid transporting layer (18), that is capable of being placed in contact with a meter (28), said layer having an opening (24) therein through which light can be transmitted; (5) a layer (28), disposed on the surface of the meter-contacting layer (20) that faces the skin-contacting layer (15), that is capable of detecting the presence of analyte or measuring the amount of analyte in the fluid. In order to use the multiple-layer (18) element (10), light from a source of light is transmitted through the opening (24) in the multiple-layer material to be absorbed at a light-absorbing target (22) on the skin-contacting layer (15). This light transfers energy to the target (22), and this transferred energy causes an opening to form in the skin-contacting layer (15) and an opening to form in the stratum corneum. Interstitial fluid exudes from the opening in the stratum corneum and contacts the interstitial fluid transporting layer (18). The interstitial fluid then moves along or through the interstitial fluid transporting layer (18) to the detecting layer (28).

公开(公告)号：EP0923735B1

公开(公告)日：2002-08-28

申请号：EP97926857.0

申请日：1997-06-03

Applicant: Abbott Laboratories

Inventor： POPE, Mark, R. ,  TARCHA, Peter, J. ,  MEES, David, R. ,  JOSEPH, Mary, K. ,  PRY, Terry, A. ,  PUTMAN, C., Brent ,  SUBOTICH, Daniel, T.

IPC: G01N33/543 ,  G01N33/545 ,  G01N33/546

CPC classification number: G01N33/54393 ,  G01N33/54353 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S436/81 ,  Y10S436/823

Abstract: A method for increasing the binding activity of specific binding members bound to a solid phase material, e.g., a particle, that has been sterically stabilized. This increase in binding activity is brought about by degrading a steric stabilizer on the surface of the solid phase material. The method involves both immobilizing a specific binding member on the surface of a solid phase material and degrading a steric stabilizer on the surface of that solid phase material. In the preferred embodiment, the method involves the immobilization of a specific binding member on the surface of the sterically stabilized solid phase material, with subsequent degradation of the steric stabilizer.

公开(公告)号：EP1011490A1

公开(公告)日：2000-06-28

申请号：EP98911910.2

申请日：1998-03-20

Applicant: Abbott Laboratories

Inventor： LOOMIS, Neil, W. ,  BOJAN, Peter, M. ,  HENNING, Timothy, P. ,  POPE, Mark, R. ,  MUETTERTIES, Andrew, J.

IPC: A61B17/36

CPC classification number: A61B18/20 ,  A61B5/14514 ,  A61B5/150022 ,  A61B5/15136 ,  A61B2017/00765 ,  A61B2018/00636 ,  A61B2018/1807 ,  A61M2037/0007

Abstract: A method for increasing the permeability of the stratum corneum by means of a source of light, preferably a laser, more preferably a pulsed laser. By increasing the permeability of the stratum corneum, access to the interstitial fluid is achieved, thereby enabling measurement of analytes in the interstitial fluid. In one aspect, the method comprises the steps of (a) providing a source of light having a wavelength of from about 930 nm to about 1040 nm; and (b) exposing a region of the stratum corneum of the patient to said source of light for a period of time sufficient to form an opening in the stratum corneum. Preferably, exposure of the region of the stratum corneum to the source of light is ceased when an amount of interstitial fluid fills the opening in the stratum corneum, which amount is sufficient to cause the scatter intensity of the light reflected from the surface of the interstitial fluid occupying the opening in the stratum corneum to differ from the scatter intensity of the light reflected from a region of the stratum corneum that is substantially free of interstitial fluid. The invention also involves an apparatus for carrying out the foregoing method.