公开(公告)号：WO2011078980A1

公开(公告)日：2011-06-30

申请号：PCT/US2010/059885

申请日：2010-12-10

Applicant: ABBOTT LABORATORIES ,  KENDALL, Richard ,  ROCHE, John, W.

Inventor： KENDALL, Richard ,  ROCHE, John, W.

IPC: G01N33/72 ,  G01N1/28 ,  G01N15/14

CPC classification number: G01N15/1434 ,  G01N1/2813 ,  G01N15/1475 ,  G01N21/27 ,  G01N21/49 ,  G01N21/59 ,  G01N33/721 ,  G01N2001/282 ,  G01N2015/0073 ,  G01N2015/008 ,  G01N2015/1486 ,  G01N2333/805

Abstract: A method for counting blood cells in a sample of whole blood. The method comprises the steps of: (a) providing a sample of whole blood; (b) depositing the sample of whole blood onto a slide, e.g., a microscope slide; (c) employing a spreader to create a blood smear; (d) allowing the blood smear to dry on the slide; (e) measuring absorption or reflectance of light attributable to the hemoglobin in the red blood cells in the blood smear on the slide; (f) recording a magnified two-dimensional digital image of the area of analysis identified by the measurement in step (e) as being of suitable thickness for analysis; and (g) collecting, analyzing, and storing data from the magnified two-dimensional digital image. Optionally, steps of fixing and staining of blood cells on the slide can be employed in the method.

Abstract translation: 一种全血样品中血细胞计数的方法。 该方法包括以下步骤：（a）提供全血样品; （b）将全血样品沉积在载玻片上，例如显微镜载片上; （c）使用吊具产生血液涂片; （d）允许血液涂片在载玻片上干燥; （e）测量归因于载玻片上血液涂片中红细胞中血红蛋白的光的吸光度或反射率; （f）将通过步骤（e）中的测量识别的分析区域的放大二维数字图像记录为具有适于分析的厚度; 和（g）从放大的二维数字图像中收集，分析和存储数据。 任选地，可以在该方法中使用在载玻片上固定和染色血细胞的步骤。

公开(公告)号：WO1993016384A1

公开(公告)日：1993-08-19

申请号：PCT/US1993000917

申请日：1993-02-03

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  VON BEHRENS, Wieland, E. ,  HAIFLICH, Sherry ,  GLAZIER, John ,  ROCHE, John, W. ,  DIRECTOR, Bruce, A.

IPC: G01N33/48

CPC classification number: G01N33/5094 ,  G01N15/1434 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1413 ,  G01N2015/1477 ,  Y10T436/107497

Abstract: A method for identifying, characterizing, categorizing and enumerating cells. The method is based on the survival of different cell populations in the sample when the sample condition is being changed to elicit a response. The cells are being monitored either by direct disappearance of intact cells or by the appearance of cell structures, carcasses, ghosts or residuum. In one embodiment, a leukocyte cell decay rate in the presence of an erythrolytic agent is determined by monitoring leukocyte counts at several time intervals. The decay rate is used to determine the presence of fragile leukocyte and the number of leukocytes in the sample. The method corrects errors in leukocyte counts which enables the use of strong erythrolytic agents in presence of lyse-resistant erythrocytes. The leukocyte decay rate can be back extrapolated to time zero to provide an accurate estimate of the leukocyte count initially present in the sample.

Abstract translation: 用于识别，表征，分类和枚举单元格的方法。 该方法基于当样品条件改变以引发响应时​​样品中不同细胞群体的存活。 正在通过完整细胞的直接消失或细胞结构，尸体，鬼魂或残渣的出现来监测细胞。 在一个实施方案中，通过以几个时间间隔监测白细胞计数来确定在存在红细胞分解剂的情况下的白细胞细胞衰变速率。 衰减率用于确定样品中易碎白细胞的存在和白细胞数。 该方法校正白细胞计数中的错误，这使得在溶血性红细胞存在下使用强烈的红血球药物。 白细胞衰减速率可以反向外推到时间零，以提供样品中最初存在的白细胞计数的准确估计。

公开(公告)号：EP2517023B1

公开(公告)日：2014-07-23

申请号：EP10793400.2

申请日：2010-12-10

Applicant: Abbott Laboratories

Inventor： KENDALL, Richard ,  ROCHE, John, W.

IPC: G01N33/72 ,  G01N1/28 ,  G01N15/14

CPC classification number: G01N15/1434 ,  G01N1/2813 ,  G01N15/1475 ,  G01N21/27 ,  G01N21/49 ,  G01N21/59 ,  G01N33/721 ,  G01N2001/282 ,  G01N2015/0073 ,  G01N2015/008 ,  G01N2015/1486 ,  G01N2333/805

Abstract: A method for counting blood cells in a sample of whole blood. The method comprises the steps of: (a) providing a sample of whole blood; (b) depositing the sample of whole blood onto a slide, e.g., a microscope slide; (c) employing a spreader to create a blood smear; (d) allowing the blood smear to dry on the slide; (e) measuring absorption or reflectance of light attributable to the hemoglobin in the red blood cells in the blood smear on the slide; (f) recording a magnified two-dimensional digital image of the area of analysis identified by the measurement in step (e) as being of suitable thickness for analysis; and (g) collecting, analyzing, and storing data from the magnified two-dimensional digital image. Optionally, steps of fixing and staining of blood cells on the slide can be employed in the method.

公开(公告)号：EP0626067A1

公开(公告)日：1994-11-30

申请号：EP93904824.0

申请日：1993-02-03

Applicant: ABBOTT LABORATORIES

Inventor： VON BEHRENS, Wieland, E. ,  HAIFLICH, Sherry ,  GLAZIER, John ,  ROCHE, John, W. ,  DIRECTOR, Bruce, A.

IPC: G01N33 ,  C12M1 ,  G01N15

CPC classification number: G01N33/5094 ,  G01N15/1434 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1413 ,  G01N2015/1477 ,  Y10T436/107497

Abstract: L'invention concerne un procédé destiné à identifier, à distinguer, à classer par catégories et à dénombrer des cellules. Le procédé est basé sur la durée de vie de différentes populations cellulaires dans un échantillon dont on est train de modifier les conditions pour induire une réponse. Ces cellules sont contrôlées soit par élimination directe des cellules intactes soit par introduction de structures cellulaires, de carcasses, d'hôtes ou de résidu. Dans un mode de réalisation, le taux de désintégration des cellules leucocytaires en présence d'un agent érythrolytique est déterminé en contrôlant la numération leucocytaire à plusieur intervalles de temps. Le taux de désintégration est utilisé afin de déterminer la présence des leucocytes dans l'échantillon. Le procédé corrige les erreurs de comptage des leucocytes et permet d'utiliser de puissants agents érythrolytiques en présence d'hérythrocytes résistant à la lyse. Le taux de désintégration des leucocytes peut être rétroextrapolé à zéro pour donner une estimation précise du comptage des leucocytes initialement présents dans l'échantillon.

公开(公告)号：EP2517023A1

公开(公告)日：2012-10-31

申请号：EP10793400.2

申请日：2010-12-10

Applicant: Abbott Laboratories

Inventor： KENDALL, Richard ,  ROCHE, John, W.

IPC: G01N33/72 ,  G01N1/28 ,  G01N15/14

CPC classification number: G01N15/1434 ,  G01N1/2813 ,  G01N15/1475 ,  G01N21/27 ,  G01N21/49 ,  G01N21/59 ,  G01N33/721 ,  G01N2001/282 ,  G01N2015/0073 ,  G01N2015/008 ,  G01N2015/1486 ,  G01N2333/805

Abstract: A method for counting blood cells in a sample of whole blood. The method comprises the steps of: (a) providing a sample of whole blood; (b) depositing the sample of whole blood onto a slide, e.g., a microscope slide; (c) employing a spreader to create a blood smear; (d) allowing the blood smear to dry on the slide; (e) measuring absorption or reflectance of light attributable to the hemoglobin in the red blood cells in the blood smear on the slide; (f) recording a magnified two-dimensional digital image of the area of analysis identified by the measurement in step (e) as being of suitable thickness for analysis; and (g) collecting, analyzing, and storing data from the magnified two-dimensional digital image. Optionally, steps of fixing and staining of blood cells on the slide can be employed in the method.