公开(公告)号：EP0288793A2

公开(公告)日：1988-11-02

申请号：EP88105533.9

申请日：1988-04-07

Applicant: ABBOTT LABORATORIES

Inventor： Brown, William E. III ,  Sebesta, Edward E. ,  Osikowicz, Eugene W. ,  Ramp, Sally K. ,  Grandone, Cass J.

IPC: B01L3/00 ,  G01N33/50

CPC classification number: B01L3/502 ,  G01N33/54366 ,  G01N35/025

Abstract: A disposable cartridge suitable for performing automated solid-phase diagnostic assays which employs microparticles to complex an analyte and where the microparticle complex becomes retained and immobilized on a fibrous matrix such that the presence of analyte on the microparticles can be detected by optical means. A cartridge is disclosed which comprises a plurality of wells at least one of the wells comprises an entrance port and holding means for receiving and holding a quantity of sample and assay reagents, a fibrous matrix for retaining and immobilizing microparticle/analyte complexes for detection, the fibrous matrix positioned below the holding means, and having an average spatial separation of fibers greater than the average diameter of said microparticles, and an absorbent material positioned below the fibrous matrix to absorb any sample or assay reagents that flow through said fibrous matrix.

Abstract translation: 一种适用于执行自动化固相诊断测定的一次性盒，其使用微粒来复合分析物，并且其中微粒复合物保留并固定在纤维基质上，使得可以通过光学方法检测微粒上分析物的存在。 公开了一种盒，其包括多个孔，至少一个孔包括入口和用于接收和保持一定量的样品和测定试剂的保持装置，用于保持和固定微粒/分析物复合物进行检测的纤维基质， 纤维基质定位在保持装置下方，并且具有大于所述微粒平均直径的纤维的平均空间分离，以及位于纤维基质下方的吸收材料，以吸收流过所述纤维基质的任何样品或测定试剂。

公开(公告)号：EP0288793A3

公开(公告)日：1990-04-25

申请号：EP88105533.9

申请日：1988-04-07

Applicant: ABBOTT LABORATORIES

Inventor： Brown, William E. III ,  Sebesta, Edward E. ,  Osikowicz, Eugene W. ,  Ramp, Sally K. ,  Grandone, Cass J.

IPC: B01L3/00 ,  G01N33/50

CPC classification number: B01L3/502 ,  G01N33/54366 ,  G01N35/025

Abstract: A disposable cartridge suitable for performing automated solid-phase diagnostic assays which employs microparticles to complex an analyte and where the microparticle complex becomes retained and immobilized on a fibrous matrix such that the presence of analyte on the microparticles can be detected by optical means. A cartridge is disclosed which comprises a plurality of wells at least one of the wells comprises an entrance port and holding means for receiving and holding a quantity of sample and assay reagents, a fibrous matrix for retaining and immobilizing microparticle/analyte complexes for detection, the fibrous matrix positioned below the holding means, and having an average spatial separation of fibers greater than the average diameter of said microparticles, and an absorbent material positioned below the fibrous matrix to absorb any sample or assay reagents that flow through said fibrous matrix.

Abstract translation: 一种适用于执行自动化固相诊断测定的一次性盒，其使用微粒来复合分析物，并且其中微粒复合物保留并固定在纤维基质上，使得可以通过光学方法检测微粒上分析物的存在。 公开了一种盒，其包括多个孔，至少一个孔包括入口和用于接收和保持一定量的样品和测定试剂的保持装置，用于保持和固定微粒/分析物复合物进行检测的纤维基质， 纤维基质定位在保持装置下方，并且具有大于所述微粒平均直径的纤维的平均空间分离，以及位于纤维基质下方的吸收材料，以吸收流过所述纤维基质的任何样品或测定试剂。