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1.发明公开FORMATION OF AND METHODS FOR THE PRODUCTION OF LARGE $i(BACILLUS THURINGIENSIS) CRYSTALS WITH INCREASED PESTICIDAL ACTIVITY 失效地层中，生成荚大苏云金芽孢杆菌晶体更高的杀虫活性的方法
公开(公告)号：EP0708834A1
公开(公告)日：1996-05-01
申请号：EP94922566.0
申请日：1994-07-14
Applicant: Abbott Laboratories
Inventor： ADAMS, Lee, Fremont , THOMAS, Michael, David , SLOMA, Alan, P. , WIDNER, William R., Apartment 301
CPC classification number: A01N63/00 , C07K14/325 , C12N15/75
Abstract: The invention relates to a method for producing an integrant(s) of Bacillus thuringiensis which produces a larger quantity of a crystal delta-endotoxin with greater pesticidal activity as compared to the crystal delta-endotoxin produced by the corresponding parental strain. The crystal delta-endotoxin produced by the integrant Bacillus thuringiensis will have an activity directed towards the same pest(s) as its parent Bacillus thuringiensis crystal delta-endotoxin. The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.
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2.发明授权NOVEL $i(BACILLUS THURINGIENSIS) STRAINS ACTIVE AGAINST LEPIDOPTERAN AND COLEOPTERAN PESTS 失效新型I（苏云金芽孢杆菌）ACTIVE $ STEM反对鳞翅目和鞘翅目
公开(公告)号：EP0674704B1
公开(公告)日：2003-03-05
申请号：EP94905931.5
申请日：1993-12-13
Applicant: ABBOTT LABORATORIES
Inventor： LIU, Chi-Li , ADAMS, Lee, Fremont , LUFBURROW, Patricia, A. , THOMAS, Michael, David
CPC classification number: C07K14/325 , A01N63/00
Abstract: The invention is related to a novel biologically pure Bacillus thuringiensis (B.t.) strain active against lepidopteran and coleopteran pests which produces a bipyramidal crystal consisting essentially of a delta-endotoxin having a molecular weight of about 130,000 daltons and a rhomboidal crystal consisting essentially of two delta-endotoxins, each having a molecular weight of about 33,000 daltons, as well as spores, crystals, delta-endotoxins and/or mutants thereof. The invention also relates to insecticidal compositions obtainable therefrom. The invention further relates to methods of using the insecticidal compositions to control insect pest from the order Lepidoptera and/or Coleoptera. The invention also relates to isolated DNA sequences encoding the delta-endotoxins.
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3.发明公开NOVEL BACILLUS THURINGIENSIS STRAINS ACTIVE AGAINST LEPIDOPTERAN AND COLEOPTERAN PESTS 失效新的苏云金芽孢杆菌AGAINST鳞翅目害虫和KOLEOPTEREN活跃程度
公开(公告)号：EP0766738A1
公开(公告)日：1997-04-09
申请号：EP95923054.0
申请日：1995-06-15
Applicant: ABBOTT LABORATORIES
Inventor： LIU, Chi-Li , ADAMS, Lee, Fremont , LUFBURROW, Patricia, A. , THOMAS, Michael, David
CPC classification number: A01N63/00 , C07K14/325
Abstract: The invention is related to a novel delta-endotoxins, each having a molecular weight of about 33,000. The delta-endotoxins are obtainable from a novel biologically pure Bacillus thuringiensis (B.t.) strains active against lepidopteran and coleopteran pests which produces a bipyramidal crystal consisting essentially of at least two delta-endotoxins having a molecular weight of about 130,000 daltons and a rhomboidal crystal consisting essentially of two delta-endotoxins, each having a molecular weight of about 33,000 daltons and/or mutants thereof. The invention also relates to insecticidal compositions obtainable therefrom. The invention further relates to methods of using the insecticidal compositions to control an insect pest(s) from the order Lepidoptera and/or Coleoptera. The invention also relates to isolated nucleic acid sequences encoding the delta-endotoxins and probes for detecting such nucleic acid sequences.
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