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公开(公告)号：WO1995011437A1
公开(公告)日：1995-04-27
申请号：PCT/US1994011016
申请日：1994-09-28
Applicant: ABBOTT LABORATORIES
Inventor： ABBOTT LABORATORIES , ZAUN, Peter , BOUMA, Stanley, R. , GORDON, Julian , KOTLARIK, John, J. , SOLOMON, Natalie, A.
IPC: G01N01/00
CPC classification number: B01L7/52 , B01L3/502 , G01N35/1095 , G01N2035/00237 , G01N2035/00366 , G01N2035/00376 , G01N2035/00752 , Y10S435/808
Abstract: Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a one or two-tier thermal cycling device (16) that operates in conjunction with a reaction/detection unit (20). A sample (38) is loaded into a reaction chamber (30) of the device which is then mated with a detection chamber (32) to form the reaction/detection unit. A first heating element (90) of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element (92) and amplified target nucleic acid is immobilized on a support in the detection chamber. Microprocessor control controls the heat applied by the second element independently of the heat applied by the first element. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Images are captured and digitized and subjected to statistical analysis to improve the accuracy of the method.
Abstract translation: 提供了用于扩增和检测核酸的方法，装置，装置和试剂盒。 该装置是与反应/检测单元（20）一起操作的一层或两层热循环装置（16）。 将样品（38）装载到装置的反应室（30）中，然后与检测室（32）配合以形成反应/检测单元。 热循环装置的第一加热元件（90）对反应/检测装置施加期望的温度以放大样品中的靶核酸。 然后将反应混合物通过第二加热元件（92）转移到检测室，并将扩增的靶核酸固定在检测室中的载体上。 微处理器控制独立于第一元件施加的热量来控制由第二元件施加的热量。 与该装置相关的检测系统检测并分析固定的扩增的核酸靶。 图像被捕获并数字化并进行统计分析以提高该方法的准确性。
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公开(公告)号：EP0724717B1
公开(公告)日：1998-08-19
申请号：EP94931783.8
申请日：1994-09-28
Applicant: Abbott Laboratories
Inventor： ZAUN, Peter , BOUMA, Stanley, R. , GORDON, Julian , KOTLARIK, John, J. , SOLOMON, Natalie, A.
CPC classification number: B01L7/52 , B01L3/502 , G01N35/1095 , G01N2035/00237 , G01N2035/00366 , G01N2035/00376 , G01N2035/00752 , Y10S435/808
Abstract: Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a one or two-tier thermal cycling device (16) that operates in conjunction with a reaction/detection unit (20). A sample (38) is loaded into a reaction chamber (30) of the device which is then mated with a detection chamber (32) to form the reaction/detection unit. A first heating element (90) of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element (92) and amplified target nucleic acid is immobilized on a support in the detection chamber. Microprocessor control controls the heat applied by the second element independently of the heat applied by the first element. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Images are captured and digitized and subjected to statistical analysis to improve the accuracy of the method.
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公开(公告)号：EP0724717A1
公开(公告)日：1996-08-07
申请号：EP94931783.0
申请日：1994-09-28
Applicant: Abbott Laboratories
Inventor： ZAUN, Peter , BOUMA, Stanley, R. , GORDON, Julian , KOTLARIK, John, J. , SOLOMON, Natalie, A.
CPC classification number: B01L7/52 , B01L3/502 , G01N35/1095 , G01N2035/00237 , G01N2035/00366 , G01N2035/00376 , G01N2035/00752 , Y10S435/808
Abstract: Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a one or two-tier thermal cycling device (16) that operates in conjunction with a reaction/detection unit (20). A sample (38) is loaded into a reaction chamber (30) of the device which is then mated with a detection chamber (32) to form the reaction/detection unit. A first heating element (90) of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element (92) and amplified target nucleic acid is immobilized on a support in the detection chamber. Microprocessor control controls the heat applied by the second element independently of the heat applied by the first element. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Images are captured and digitized and subjected to statistical analysis to improve the accuracy of the method.
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