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    WIDE DYNAMIC RANGE NUCLEIC ACID DETECTION USING AN AGGREGATE PRIMER SERIES
    1.
    发明申请
    WIDE DYNAMIC RANGE NUCLEIC ACID DETECTION USING AN AGGREGATE PRIMER SERIES 审中-公开
    使用聚合引物系列进行动态范围的核酸检测

    公开(公告)号：WO1996036736A2

    公开(公告)日：1996-11-21

    申请号：PCT/US1996007138

    申请日：1996-05-17

    Applicant: ABBOTT LABORATORIES

    Inventor： ABBOTT LABORATORIES ,  SOLOMON, Natalie, A. ,  BOUMA, Stanley, R.

    IPC: C12Q01/68

    CPC classification number: C12Q1/6851 ,  C12Q1/6834 ,  C12Q1/6862 ,  C12Q1/703 ,  C12Q1/706 ,  C12Q2537/143 ,  C12Q2527/143

    Abstract: The present invention provides a method of detecting the amount of a target sequence which may be present in a test sample. The method uses an aggregate primer series, which comprises at least two primer sets, in an amplification reaction to detect the relative concentration of a target sequence which may be present in a test sample. The primer sets have different sensitivities and hybridize with sub-target sequences which are different regions of the target sequence. The method generally comprises cycling a test sample suspected of containing a target sequence, an aggregate primer series, and means necessary for performing an amplification reaction; and detecting any amplified sub-target sequences. Based on a qualitative detection of the amplified sub-target sequences generated by individual primer sets, the relative quantity of the target sequence can be determined.

    Abstract translation: 本发明提供了检测可能存在于测试样品中的靶序列的量的方法。 该方法在扩增反应中使用包含至少两个引物组的聚集引物序列，以检测可能存在于测试样品中的靶序列的相对浓度。 引物组具有不同的灵敏度并与靶序列的不同区域的亚靶序列杂交。 该方法通常包括循环怀疑含有靶序列的测试样品，聚集引物序列和进行扩增反应所需的手段; 并检测任何扩增的亚靶序列。 基于由各引物组产生的扩增子靶序列的定性检测，可以确定目标序列的相对量。

    APPARATUS AND METHOD FOR TRANSFER OF A FLUID SAMPLE
    2.
    发明申请
    APPARATUS AND METHOD FOR TRANSFER OF A FLUID SAMPLE 审中-公开
    用于转移流体样品的装置和方法

    公开(公告)号：WO1995011437A1

    公开(公告)日：1995-04-27

    申请号：PCT/US1994011016

    申请日：1994-09-28

    Applicant: ABBOTT LABORATORIES

    Inventor： ABBOTT LABORATORIES ,  ZAUN, Peter ,  BOUMA, Stanley, R. ,  GORDON, Julian ,  KOTLARIK, John, J. ,  SOLOMON, Natalie, A.

    IPC: G01N01/00

    CPC classification number: B01L7/52 ,  B01L3/502 ,  G01N35/1095 ,  G01N2035/00237 ,  G01N2035/00366 ,  G01N2035/00376 ,  G01N2035/00752 ,  Y10S435/808

    Abstract: Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a one or two-tier thermal cycling device (16) that operates in conjunction with a reaction/detection unit (20). A sample (38) is loaded into a reaction chamber (30) of the device which is then mated with a detection chamber (32) to form the reaction/detection unit. A first heating element (90) of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element (92) and amplified target nucleic acid is immobilized on a support in the detection chamber. Microprocessor control controls the heat applied by the second element independently of the heat applied by the first element. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Images are captured and digitized and subjected to statistical analysis to improve the accuracy of the method.

    Abstract translation: 提供了用于扩增和检测核酸的方法，装置，装置和试剂盒。 该装置是与反应/检测单元（20）一起操作的一层或两层热循环装置（16）。 将样品（38）装载到装置的反应室（30）中，然后与检测室（32）配合以形成反应/检测单元。 热循环装置的第一加热元件（90）对反应/检测装置施加期望的温度以放大样品中的靶核酸。 然后将反应混合物通过第二加热元件（92）转移到检测室，并将扩增的靶核酸固定在检测室中的载体上。 微处理器控制独立于第一元件施加的热量来控制由第二元件施加的热量。 与该装置相关的检测系统检测并分析固定的扩增的核酸靶。 图像被捕获并数字化并进行统计分析以提高该方法的准确性。

    APPARATUS AND METHOD FOR TRANSFER OF A FLUID SAMPLE
    8.
    发明授权
    APPARATUS AND METHOD FOR TRANSFER OF A FLUID SAMPLE 失效
    设备和方法将样品置于流体

    公开(公告)号：EP0724717B1

    公开(公告)日：1998-08-19

    申请号：EP94931783.8

    申请日：1994-09-28

    Applicant: Abbott Laboratories

    Inventor： ZAUN, Peter ,  BOUMA, Stanley, R. ,  GORDON, Julian ,  KOTLARIK, John, J. ,  SOLOMON, Natalie, A.

    IPC: G01N1/00 ,  G01N35/00 ,  B01L7/00 ,  B01L3/00 ,  C12Q1/68

    CPC classification number: B01L7/52 ,  B01L3/502 ,  G01N35/1095 ,  G01N2035/00237 ,  G01N2035/00366 ,  G01N2035/00376 ,  G01N2035/00752 ,  Y10S435/808

    Abstract: Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a one or two-tier thermal cycling device (16) that operates in conjunction with a reaction/detection unit (20). A sample (38) is loaded into a reaction chamber (30) of the device which is then mated with a detection chamber (32) to form the reaction/detection unit. A first heating element (90) of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element (92) and amplified target nucleic acid is immobilized on a support in the detection chamber. Microprocessor control controls the heat applied by the second element independently of the heat applied by the first element. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Images are captured and digitized and subjected to statistical analysis to improve the accuracy of the method.

    APPARATUS AND METHOD FOR TRANSFER OF A FLUID SAMPLE
    9.
    发明公开
    APPARATUS AND METHOD FOR TRANSFER OF A FLUID SAMPLE 失效
    设备和方法将样品置于流体

    公开(公告)号：EP0724717A1

    公开(公告)日：1996-08-07

    申请号：EP94931783.0

    申请日：1994-09-28

    Applicant: Abbott Laboratories

    Inventor： ZAUN, Peter ,  BOUMA, Stanley, R. ,  GORDON, Julian ,  KOTLARIK, John, J. ,  SOLOMON, Natalie, A.

    IPC: B01L3 ,  B01L7 ,  B01L11 ,  C12N15 ,  C12Q1 ,  G01N1 ,  G01N35

    CPC classification number: B01L7/52 ,  B01L3/502 ,  G01N35/1095 ,  G01N2035/00237 ,  G01N2035/00366 ,  G01N2035/00376 ,  G01N2035/00752 ,  Y10S435/808

    Abstract: Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a one or two-tier thermal cycling device (16) that operates in conjunction with a reaction/detection unit (20). A sample (38) is loaded into a reaction chamber (30) of the device which is then mated with a detection chamber (32) to form the reaction/detection unit. A first heating element (90) of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element (92) and amplified target nucleic acid is immobilized on a support in the detection chamber. Microprocessor control controls the heat applied by the second element independently of the heat applied by the first element. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Images are captured and digitized and subjected to statistical analysis to improve the accuracy of the method.

    WIDE DYNAMIC RANGE NUCLEIC ACID DETECTION USING AN AGGREGATE PRIMER SERIES
    10.
    发明授权
    WIDE DYNAMIC RANGE NUCLEIC ACID DETECTION USING AN AGGREGATE PRIMER SERIES 失效
    NUCLEIC广泛的检测利用装置，底漆系列

    公开(公告)号：EP0826069B1

    公开(公告)日：2002-04-10

    申请号：EP96920266.2

    申请日：1996-05-17

    Applicant: Abbott Laboratories

    Inventor： SOLOMON, Natalie, A. ,  BOUMA, Stanley, R.

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/6851 ,  C12Q1/6834 ,  C12Q1/6862 ,  C12Q1/703 ,  C12Q1/706 ,  C12Q2537/143 ,  C12Q2527/143

    Abstract: The present invention provides a method of detecting the amount of a target sequence which may be present in a test sample. The method uses an aggregate primer series, which comprises at least two primer sets, in an amplification reaction to detect the relative concentration of a target sequence which may be present in a test sample. The primer sets have different sensitivities and hybridize with sub-target sequences which are different regions of the target sequence. The method generally comprises cycling a test sample suspected of containing a target sequence, an aggregate primer series, and means necessary for performing an amplification reaction; and detecting any amplified sub-target sequences. Based on a qualitative detection of the amplified sub-target sequences generated by individual primer sets, the relative quantity of the target sequence can be determined.

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