公开(公告)号：JP2005185291A

公开(公告)日：2005-07-14

申请号：JP2005053906

申请日：2005-02-28

Applicant: Academia Sinica ,  アカデミア・シニカAcademia Sinica

Inventor： CHEI-FUU SHOO ,  KUAN-CHIUN LEE ,  SHII-CHIE TAN

IPC: C12N15/09 ,  C12N9/20

Abstract: PROBLEM TO BE SOLVED: To provide a nucleic acid that can be used to functionally express a heterologous C. rugosa lipase in a common host cell, a lipase having a specific property for industrial applications and a microorganism capable of producing the lipase. SOLUTION: This isolated nucleic acid comprises a mutant DNA encoding a Candida rugosa lipase, wherein the mutant DNA is at least 80% identical to a wild-type DNA encoding the Candida rugosa lipase, and includes at least 12 codons corresponding to CTG codon in the wild-type DNA, each of the 12 codons, independently, being TCT, TCC, TCA, TCG, AGT, or AGC. A chimeric Candida rugosa lipase comprises a substrate interacting domain of a first C. rugosa lipase and a substrate interacting domain of a second C. rugosa lipase. This C. rugosa lipase is encoded by the nucleic acid. This microorganism comprises the nucleic acid. COPYRIGHT: (C)2005,JPO&NCIPI

公开(公告)号：JP2005185290A

公开(公告)日：2005-07-14

申请号：JP2005053891

申请日：2005-02-28

Applicant: Academia Sinica ,  アカデミア・シニカAcademia Sinica

Inventor： CHEI-FUU SHOO ,  KUAN-CHIUN LEE ,  SHII-CHIE TAN

IPC: C12N15/09 ,  C12N1/19 ,  C12N1/21 ,  C12N9/20

Abstract: PROBLEM TO BE SOLVED: To provide a nucleic acid that can be used to functionally express a heterologous C. rugosa lipase in a common host cell, a lipase having a specific property for industrial applications and a microorganism capable of producing the lipase. SOLUTION: This isolated nucleic acid comprises a mutant DNA encoding a Candida rugosa lipase, wherein the mutant DNA is at least 80% identical to a wild-type DNA encoding the Candida rugosa lipase, and includes at least 12 codons corresponding to CTG codon in the wild-type DNA, each of the 12 codons, independently, being TCT, TCC, TCA, TCG, AGT, or AGC. A chimeric Candida rugosa lipase comprises a substrate interacting domain of a first C. rugosa lipase and a substrate interacting domain of a second C. rugosa lipase. This C. rugosa lipase is encoded by the nucleic acid. This microorganism comprises the nucleic acid. COPYRIGHT: (C)2005,JPO&NCIPI

Abstract translation: 待解决的问题：提供可用于在常见宿主细胞中功能性表达异源的C. rugosa脂肪酶的核酸，对于工业应用具有特异性的脂肪酶和能够产生脂肪酶的微生物。 解决方案：该分离的核酸包含编码假丝酵母假单胞菌脂肪酶的突变型DNA，其中突变DNA与编码假丝酵母脂肪酶的野生型DNA至少80％相同，并且包括至少12个对应于CTG的密码子 野生型DNA中的密码子，12个密码子中的每一个独立地为TCT，TCC，TCA，TCG，AGT或AGC。 嵌合假丝酵母属脂肪酶包含第一C.Rollosa脂肪酶的底物相互作用结构域和第二种C. rugosa脂肪酶的底物相互作用结构域。 这种C. rugosa脂肪酶是由核酸编码的。 该微生物包含核酸。 版权所有（C）2005，JPO＆NCIPI

公开(公告)号：JP2003144162A

公开(公告)日：2003-05-20

申请号：JP2001328304

申请日：2001-10-25

Applicant: ACADEMIA SINICA

Inventor： CHEI-FUU SHOO ,  KUAN-CHIUN LEE ,  SHII-CHIE TAN

IPC: C12N15/09 ,  C12N1/19 ,  C12N1/21 ,  C12N9/20

Abstract: PROBLEM TO BE SOLVED: To provide a nucleic acid that can be used to functionally express a heterologous C. rugosa lipase in a common host cell, a lipase having a specific property for industrial applications and a microorganism capable of producing the lipase. SOLUTION: This isolated nucleic acid comprises a mutant DNA encoding a Candida rugosa lipase, wherein the mutant DNA is at least 80% identical to a wild-type DNA encoding the Candida rugosa lipase, and includes at least 12 codons corresponding to CTG codons in the wild-type DNA, each of the 12 codons, independently, being TCT, TCC, TCA, TCG, AGT, or AGC. A chimeric Candida rugosa lipase comprises a substrate interacting domain of a first C. rugosa lipase and a non-substrate interacting domain of a second C. rugosa lipase. This C. rugosa lipase is encoded by the nucleic acid. This microorganism comprises the nucleic acid.