公开(公告)号：JPH1066598A

公开(公告)日：1998-03-10

申请号：JP21235396

申请日：1996-08-12

Applicant: ACADEMIA SINICA

Inventor： KONAN PEC ,  PANCHIRU YAN ,  SHIYURI UON

IPC: C12Q1/18 ,  C12Q1/68

Abstract: PROBLEM TO BE SOLVED: To provide a method for rapid identification and determination of effects of an antimicrobial agent. SOLUTION: This method for test comprises the following steps (a) to (d): (a) incubating a specimen of a microorganism in a culture medium containing an antimicrobial agent at a continuously diluted concentration in a short time and providing the different total numbers of microorganisms, (b) amplifying the total different numbers of microorganisms according to an in vitro microbial DNA replication, (c) adding a reporter molecule, specific for a double-stranded DNA (dsDNA) and capable of forming a complex therewith to the resultant DNA amplification products and (d) observing the presence or absence of a signal release from the reporter molecule-dsDNA complex.